Furosemide in water matrix: HPLC-UV method development and degradation studies,Furosemida em matriz de água: Desenvolvimento de um método HPLC-UV e estudos de degradação
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/30007 https://doi.org/10.4136/ambi-agua.2406 |
Resumo: | This study developed a method for furosemide quantification through high performance liquid chromatographic technique. Special attention was given to solute loss and storage stability due to furosemide’s low solubility and photosensitivity, respectively. The performance of Nylon and PVDF filters was tested in a 2 mg.L-1 furosemide solution. PVDF filters showed better recovery capacity and therefore are more suitable for furosemide filtration. Over eight days, three different storage conditions were studied to access furosemide degradation susceptibility: (i) exposure to light at room temperature, (ii) storage at room temperature without exposure to light, and (iii) storage at 4ºC without exposure to light. The study demonstrated that after 48 h under natural light exposure furosemide was completely degraded. Furosemide solution stored in the dark was stable. Storage temperature did not seem to affect furosemide concentration. The study shows that the selection of more suitable filter and storage conditions for furosemide determination is crucial to avoid underestimation errors. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Furosemide in water matrix: HPLC-UV method development and degradation studies,Furosemida em matriz de água: Desenvolvimento de um método HPLC-UV e estudos de degradaçãofilter retentionpharmaceuticalphotodegradationThis study developed a method for furosemide quantification through high performance liquid chromatographic technique. Special attention was given to solute loss and storage stability due to furosemide’s low solubility and photosensitivity, respectively. The performance of Nylon and PVDF filters was tested in a 2 mg.L-1 furosemide solution. PVDF filters showed better recovery capacity and therefore are more suitable for furosemide filtration. Over eight days, three different storage conditions were studied to access furosemide degradation susceptibility: (i) exposure to light at room temperature, (ii) storage at room temperature without exposure to light, and (iii) storage at 4ºC without exposure to light. The study demonstrated that after 48 h under natural light exposure furosemide was completely degraded. Furosemide solution stored in the dark was stable. Storage temperature did not seem to affect furosemide concentration. The study shows that the selection of more suitable filter and storage conditions for furosemide determination is crucial to avoid underestimation errors.Institute for Environmental Research in Hydrographic Basins (IPABHi)2021-07-12T13:53:56Z2021-07-122020-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/30007https://doi.org/10.4136/ambi-agua.2406http://hdl.handle.net/10174/30007https://doi.org/10.4136/ambi-agua.2406engMachado, A.I.; Fragoso, R.; Dordio, A.V.M.N.B.; Duarte, E.. "Furosemide in water matrix: HPLC-UV method development and degradation studies,Furosemida em matriz de água: Desenvolvimento de um método HPLC-UV e estudos de degradação". Revista Ambiente e Agua 15:1 (2020)QUI - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científicandndavbd@uevora.ptnd304Machado, A.I.Fragoso, R.Dordio, A.V.M.N.B.Duarte, E.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:27:28Zoai:dspace.uevora.pt:10174/30007Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:19:30.473885Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Furosemide in water matrix: HPLC-UV method development and degradation studies,Furosemida em matriz de água: Desenvolvimento de um método HPLC-UV e estudos de degradação |
title |
Furosemide in water matrix: HPLC-UV method development and degradation studies,Furosemida em matriz de água: Desenvolvimento de um método HPLC-UV e estudos de degradação |
spellingShingle |
Furosemide in water matrix: HPLC-UV method development and degradation studies,Furosemida em matriz de água: Desenvolvimento de um método HPLC-UV e estudos de degradação Machado, A.I. filter retention pharmaceutical photodegradation |
title_short |
Furosemide in water matrix: HPLC-UV method development and degradation studies,Furosemida em matriz de água: Desenvolvimento de um método HPLC-UV e estudos de degradação |
title_full |
Furosemide in water matrix: HPLC-UV method development and degradation studies,Furosemida em matriz de água: Desenvolvimento de um método HPLC-UV e estudos de degradação |
title_fullStr |
Furosemide in water matrix: HPLC-UV method development and degradation studies,Furosemida em matriz de água: Desenvolvimento de um método HPLC-UV e estudos de degradação |
title_full_unstemmed |
Furosemide in water matrix: HPLC-UV method development and degradation studies,Furosemida em matriz de água: Desenvolvimento de um método HPLC-UV e estudos de degradação |
title_sort |
Furosemide in water matrix: HPLC-UV method development and degradation studies,Furosemida em matriz de água: Desenvolvimento de um método HPLC-UV e estudos de degradação |
author |
Machado, A.I. |
author_facet |
Machado, A.I. Fragoso, R. Dordio, A.V.M.N.B. Duarte, E. |
author_role |
author |
author2 |
Fragoso, R. Dordio, A.V.M.N.B. Duarte, E. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Machado, A.I. Fragoso, R. Dordio, A.V.M.N.B. Duarte, E. |
dc.subject.por.fl_str_mv |
filter retention pharmaceutical photodegradation |
topic |
filter retention pharmaceutical photodegradation |
description |
This study developed a method for furosemide quantification through high performance liquid chromatographic technique. Special attention was given to solute loss and storage stability due to furosemide’s low solubility and photosensitivity, respectively. The performance of Nylon and PVDF filters was tested in a 2 mg.L-1 furosemide solution. PVDF filters showed better recovery capacity and therefore are more suitable for furosemide filtration. Over eight days, three different storage conditions were studied to access furosemide degradation susceptibility: (i) exposure to light at room temperature, (ii) storage at room temperature without exposure to light, and (iii) storage at 4ºC without exposure to light. The study demonstrated that after 48 h under natural light exposure furosemide was completely degraded. Furosemide solution stored in the dark was stable. Storage temperature did not seem to affect furosemide concentration. The study shows that the selection of more suitable filter and storage conditions for furosemide determination is crucial to avoid underestimation errors. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-03-01T00:00:00Z 2021-07-12T13:53:56Z 2021-07-12 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/30007 https://doi.org/10.4136/ambi-agua.2406 http://hdl.handle.net/10174/30007 https://doi.org/10.4136/ambi-agua.2406 |
url |
http://hdl.handle.net/10174/30007 https://doi.org/10.4136/ambi-agua.2406 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Machado, A.I.; Fragoso, R.; Dordio, A.V.M.N.B.; Duarte, E.. "Furosemide in water matrix: HPLC-UV method development and degradation studies,Furosemida em matriz de água: Desenvolvimento de um método HPLC-UV e estudos de degradação". Revista Ambiente e Agua 15:1 (2020) QUI - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica nd nd avbd@uevora.pt nd 304 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Institute for Environmental Research in Hydrographic Basins (IPABHi) |
publisher.none.fl_str_mv |
Institute for Environmental Research in Hydrographic Basins (IPABHi) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799136676377788416 |