Microfluidic-based platform to mimic the in vivo peripheral administration of neurotropic nanoparticles

Detalhes bibliográficos
Autor(a) principal: Lopes, C
Data de Publicação: 2016
Outros Autores: Gomes, C, Neto, E, Sampaio, P, Aguiar, P, Pêgo, AP
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/10216/120744
Resumo: Aim: Propose a nanoparticle for neuron-targeted retrograde gene delivery and describe a microfluidic-based culture system to provide insight into vector performance and safety. Methods: Using compartmentalized neuron cultures we dissected nanoparticle bioactivity upon delivery taking advantage of (quantitative) bioimaging tools. Results: Targeted and nontargeted nanoparticles were internalized at axon terminals and retrogradely transported to cell bodies at similar average velocities but the former have shown an axonal flux 2.7-times superior to nontargeted nanoparticles, suggesting an improved cargo-transportation efficiency. The peripheral administration of nanoparticles to axon terminals is nontoxic as compared with their direct administration to the cell body or whole neuron. Conclusion: A neuron-targeted nanoparticle system was put forward. Microfluidic-based neuron cultures are proposed as a powerful tool to investigate nanoparticle bio-performance.
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spelling Microfluidic-based platform to mimic the in vivo peripheral administration of neurotropic nanoparticlesgene deliverymicrofluidicstargeted nanoparticlesAim: Propose a nanoparticle for neuron-targeted retrograde gene delivery and describe a microfluidic-based culture system to provide insight into vector performance and safety. Methods: Using compartmentalized neuron cultures we dissected nanoparticle bioactivity upon delivery taking advantage of (quantitative) bioimaging tools. Results: Targeted and nontargeted nanoparticles were internalized at axon terminals and retrogradely transported to cell bodies at similar average velocities but the former have shown an axonal flux 2.7-times superior to nontargeted nanoparticles, suggesting an improved cargo-transportation efficiency. The peripheral administration of nanoparticles to axon terminals is nontoxic as compared with their direct administration to the cell body or whole neuron. Conclusion: A neuron-targeted nanoparticle system was put forward. Microfluidic-based neuron cultures are proposed as a powerful tool to investigate nanoparticle bio-performance.Future Medicine20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/120744eng1743-588910.2217/nnm-2016-0247Lopes, CGomes, CNeto, ESampaio, PAguiar, PPêgo, APinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-09-27T08:01:41Zoai:repositorio-aberto.up.pt:10216/120744Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-09-27T08:01:41Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Microfluidic-based platform to mimic the in vivo peripheral administration of neurotropic nanoparticles
title Microfluidic-based platform to mimic the in vivo peripheral administration of neurotropic nanoparticles
spellingShingle Microfluidic-based platform to mimic the in vivo peripheral administration of neurotropic nanoparticles
Lopes, C
gene delivery
microfluidics
targeted nanoparticles
title_short Microfluidic-based platform to mimic the in vivo peripheral administration of neurotropic nanoparticles
title_full Microfluidic-based platform to mimic the in vivo peripheral administration of neurotropic nanoparticles
title_fullStr Microfluidic-based platform to mimic the in vivo peripheral administration of neurotropic nanoparticles
title_full_unstemmed Microfluidic-based platform to mimic the in vivo peripheral administration of neurotropic nanoparticles
title_sort Microfluidic-based platform to mimic the in vivo peripheral administration of neurotropic nanoparticles
author Lopes, C
author_facet Lopes, C
Gomes, C
Neto, E
Sampaio, P
Aguiar, P
Pêgo, AP
author_role author
author2 Gomes, C
Neto, E
Sampaio, P
Aguiar, P
Pêgo, AP
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Lopes, C
Gomes, C
Neto, E
Sampaio, P
Aguiar, P
Pêgo, AP
dc.subject.por.fl_str_mv gene delivery
microfluidics
targeted nanoparticles
topic gene delivery
microfluidics
targeted nanoparticles
description Aim: Propose a nanoparticle for neuron-targeted retrograde gene delivery and describe a microfluidic-based culture system to provide insight into vector performance and safety. Methods: Using compartmentalized neuron cultures we dissected nanoparticle bioactivity upon delivery taking advantage of (quantitative) bioimaging tools. Results: Targeted and nontargeted nanoparticles were internalized at axon terminals and retrogradely transported to cell bodies at similar average velocities but the former have shown an axonal flux 2.7-times superior to nontargeted nanoparticles, suggesting an improved cargo-transportation efficiency. The peripheral administration of nanoparticles to axon terminals is nontoxic as compared with their direct administration to the cell body or whole neuron. Conclusion: A neuron-targeted nanoparticle system was put forward. Microfluidic-based neuron cultures are proposed as a powerful tool to investigate nanoparticle bio-performance.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/10216/120744
url https://hdl.handle.net/10216/120744
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1743-5889
10.2217/nnm-2016-0247
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Future Medicine
publisher.none.fl_str_mv Future Medicine
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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