Genetic diversity and structure of the portuguese pear (Pyrus communis L.) germplasm

• Autor(a) principal: Queiroz, Álvaro
• Data de Publicação: 2019
• Outros Autores: Guimarães, Joana Bagoin, Sánchez, Claudia, Simões, Fernanda, Sousa, Rui Maia, Viegas, Wanda, Veloso, Maria Manuela
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.5/18570
• topic: pear genetic resources; SSR markers; identification; diferentiation; clones discrimination

A rich heritage of traditional pear varieties is kept in national Portuguese collections. Out of these varieties, “Rocha” dominates national pear production. Although a noticeable phenotypic variation among clones of this variety has been reported, little is known about its genetic variability, as to date molecular studies have been performed on a single “Rocha” clone. Eleven Simple Sequence Repeats (SSR) markers were used to assess the genetic diversity of 130 local cultivars, 80 of them being “Rocha” clones. The results allowed the di erentiation of 75 genotypes of which 29 are “Rocha”. Three synonyms groups and four homonymous groups of other local varieties were confirmed. A Bayesian model-based clustering approach identified two distinct clusters. Using flow cytometry, six cultivars were found to be triploids. These results show high genetic variability among “Rocha” clones. In conclusion, there is a need for di erent “Rocha” clones to be preserved to enable the correct selection of the multiplication material