The RNA phosphatase PIR-1 regulates endogenous small RNA pathways in C. elegans

Detalhes bibliográficos
Autor(a) principal: Chaves, Daniel
Data de Publicação: 2021
Outros Autores: Dai, Hui, Li, Lichao, Moresco, James J, Oh, Myung Eun, Conte, Darryl, Yates, John R, Mello, Craig C, Gu, Weifeng
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/46270
Resumo: Copyright © 2021 Elsevier Inc.
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spelling The RNA phosphatase PIR-1 regulates endogenous small RNA pathways in C. elegansRNA binding proteinsRNA phosphataseRNAiDouble-stranded RNAsEmbryogenesisGermline gene regulationGermline small RNAsmRNA regulationRegulation of triphosphorylated RNASpermatogenesisCopyright © 2021 Elsevier Inc.Eukaryotic cells regulate 50-triphosphorylated RNAs (ppp-RNAs) to promote cellular functions and prevent recognition by antiviral RNA sensors. For example, RNA capping enzymes possess triphosphatase domains that remove the g phosphates of ppp-RNAs during RNA capping. Members of the closely related PIR-1 (phosphatase that interacts with RNA and ribonucleoprotein particle 1) family of RNA polyphosphatases remove both the b and g phosphates from ppp-RNAs. Here, we show that C. elegans PIR-1 dephosphorylates ppp-RNAs made by cellular RNA-dependent RNA polymerases (RdRPs) and is required for the maturation of 26G-RNAs, Dicer-dependent small RNAs that regulate thousands of genes during spermatogenesis and embryogenesis. PIR-1 also regulates the CSR-1 22G-RNA pathway and has critical functions in both somatic and germline development. Our findings suggest that PIR-1 modulates both Dicer-dependent and Dicer-independent Argonaute pathways and provide insight into how cells and viruses use a conserved RNA phosphatase to regulate and respond to ppp-RNA species.ElsevierRepositório da Universidade de LisboaChaves, DanielDai, HuiLi, LichaoMoresco, James JOh, Myung EunConte, DarrylYates, John RMello, Craig CGu, Weifeng2021-12-29T01:30:13Z2021-02-042021-02-04T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/46270engMol Cell. 2021 Feb 4;81(3):546-557.e5.1097-276510.1016/j.molcel.2020.12.0041097-4164info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:48:30Zoai:repositorio.ul.pt:10451/46270Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:58:30.531050Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The RNA phosphatase PIR-1 regulates endogenous small RNA pathways in C. elegans
title The RNA phosphatase PIR-1 regulates endogenous small RNA pathways in C. elegans
spellingShingle The RNA phosphatase PIR-1 regulates endogenous small RNA pathways in C. elegans
Chaves, Daniel
RNA binding proteins
RNA phosphatase
RNAi
Double-stranded RNAs
Embryogenesis
Germline gene regulation
Germline small RNAs
mRNA regulation
Regulation of triphosphorylated RNA
Spermatogenesis
title_short The RNA phosphatase PIR-1 regulates endogenous small RNA pathways in C. elegans
title_full The RNA phosphatase PIR-1 regulates endogenous small RNA pathways in C. elegans
title_fullStr The RNA phosphatase PIR-1 regulates endogenous small RNA pathways in C. elegans
title_full_unstemmed The RNA phosphatase PIR-1 regulates endogenous small RNA pathways in C. elegans
title_sort The RNA phosphatase PIR-1 regulates endogenous small RNA pathways in C. elegans
author Chaves, Daniel
author_facet Chaves, Daniel
Dai, Hui
Li, Lichao
Moresco, James J
Oh, Myung Eun
Conte, Darryl
Yates, John R
Mello, Craig C
Gu, Weifeng
author_role author
author2 Dai, Hui
Li, Lichao
Moresco, James J
Oh, Myung Eun
Conte, Darryl
Yates, John R
Mello, Craig C
Gu, Weifeng
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Chaves, Daniel
Dai, Hui
Li, Lichao
Moresco, James J
Oh, Myung Eun
Conte, Darryl
Yates, John R
Mello, Craig C
Gu, Weifeng
dc.subject.por.fl_str_mv RNA binding proteins
RNA phosphatase
RNAi
Double-stranded RNAs
Embryogenesis
Germline gene regulation
Germline small RNAs
mRNA regulation
Regulation of triphosphorylated RNA
Spermatogenesis
topic RNA binding proteins
RNA phosphatase
RNAi
Double-stranded RNAs
Embryogenesis
Germline gene regulation
Germline small RNAs
mRNA regulation
Regulation of triphosphorylated RNA
Spermatogenesis
description Copyright © 2021 Elsevier Inc.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-29T01:30:13Z
2021-02-04
2021-02-04T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/46270
url http://hdl.handle.net/10451/46270
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Mol Cell. 2021 Feb 4;81(3):546-557.e5.
1097-2765
10.1016/j.molcel.2020.12.004
1097-4164
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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