Clinically Expired Platelet Concentrates as a Source of Extracellular Vesicles for Targeted Anti-Cancer Drug Delivery

Detalhes bibliográficos
Autor(a) principal: Meliciano, Ana
Data de Publicação: 2023
Outros Autores: Salvador, Daniela, Mendonça, Pedro, Louro, Ana Filipa, Serra, Margarida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/158552
Resumo: Funding Information: This research was funded by the Fundação para a Ciência e Tecnologia funded projects PEFPlateletValue (PTDC/BTM-ORG/32187/2017) and EXCELERATE (2022.10467.PTDC), iNOVA4Health-(UIDB/04462/2020 and UIDP/04462/2020), a program financially supported by FCT/Ministério da Ciência, Tecnologia e Ensino Superior, through national funds. The Associate Laboratory LS4FUTURE (LA/P/0087/2020) is also acknowledge. Publisher Copyright: © 2023 by the authors.
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spelling Clinically Expired Platelet Concentrates as a Source of Extracellular Vesicles for Targeted Anti-Cancer Drug Deliveryanti-angiogenic potentialdrug delivery systemexpired platelet concentrateshuman breast cancer cell linepaclitaxelplatelet-derived extracellular vesiclesPharmaceutical ScienceSDG 3 - Good Health and Well-beingFunding Information: This research was funded by the Fundação para a Ciência e Tecnologia funded projects PEFPlateletValue (PTDC/BTM-ORG/32187/2017) and EXCELERATE (2022.10467.PTDC), iNOVA4Health-(UIDB/04462/2020 and UIDP/04462/2020), a program financially supported by FCT/Ministério da Ciência, Tecnologia e Ensino Superior, through national funds. The Associate Laboratory LS4FUTURE (LA/P/0087/2020) is also acknowledge. Publisher Copyright: © 2023 by the authors.The short shelf life of platelet concentrates (PC) of up to 5–7 days leads to higher wastage due to expiry. To address this massive financial burden on the healthcare system, alternative applications for expired PC have emerged in recent years. Engineered nanocarriers functionalized with platelet membranes have shown excellent targeting abilities for tumor cells owing to their platelet membrane proteins. Nevertheless, synthetic drug delivery strategies have significant drawbacks that platelet-derived extracellular vesicles (pEV) can overcome. We investigated, for the first time, the use of pEV as a carrier of the anti-breast cancer drug paclitaxel, considering it as an appealing alternative to improve the therapeutic potential of expired PC. The pEV released during PC storage showed a typical EV size distribution profile (100–300 nm) with a cup-shaped morphology. Paclitaxel-loaded pEV showed significant anti-cancer effects in vitro, as demonstrated by their anti-migratory (>30%), anti-angiogenic (>30%), and anti-invasive (>70%) properties in distinct cells found in the breast tumor microenvironment. We provide evidence for a novel application for expired PC by suggesting that the field of tumor treatment research may be broadened by the use of natural carriers.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNMeliciano, AnaSalvador, DanielaMendonça, PedroLouro, Ana FilipaSerra, Margarida2023-09-30T22:22:20Z2023-032023-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/158552eng1999-4923PURE: 72698440https://doi.org/10.3390/pharmaceutics15030953info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:41:06Zoai:run.unl.pt:10362/158552Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:12.741800Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Clinically Expired Platelet Concentrates as a Source of Extracellular Vesicles for Targeted Anti-Cancer Drug Delivery
title Clinically Expired Platelet Concentrates as a Source of Extracellular Vesicles for Targeted Anti-Cancer Drug Delivery
spellingShingle Clinically Expired Platelet Concentrates as a Source of Extracellular Vesicles for Targeted Anti-Cancer Drug Delivery
Meliciano, Ana
anti-angiogenic potential
drug delivery system
expired platelet concentrates
human breast cancer cell line
paclitaxel
platelet-derived extracellular vesicles
Pharmaceutical Science
SDG 3 - Good Health and Well-being
title_short Clinically Expired Platelet Concentrates as a Source of Extracellular Vesicles for Targeted Anti-Cancer Drug Delivery
title_full Clinically Expired Platelet Concentrates as a Source of Extracellular Vesicles for Targeted Anti-Cancer Drug Delivery
title_fullStr Clinically Expired Platelet Concentrates as a Source of Extracellular Vesicles for Targeted Anti-Cancer Drug Delivery
title_full_unstemmed Clinically Expired Platelet Concentrates as a Source of Extracellular Vesicles for Targeted Anti-Cancer Drug Delivery
title_sort Clinically Expired Platelet Concentrates as a Source of Extracellular Vesicles for Targeted Anti-Cancer Drug Delivery
author Meliciano, Ana
author_facet Meliciano, Ana
Salvador, Daniela
Mendonça, Pedro
Louro, Ana Filipa
Serra, Margarida
author_role author
author2 Salvador, Daniela
Mendonça, Pedro
Louro, Ana Filipa
Serra, Margarida
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Meliciano, Ana
Salvador, Daniela
Mendonça, Pedro
Louro, Ana Filipa
Serra, Margarida
dc.subject.por.fl_str_mv anti-angiogenic potential
drug delivery system
expired platelet concentrates
human breast cancer cell line
paclitaxel
platelet-derived extracellular vesicles
Pharmaceutical Science
SDG 3 - Good Health and Well-being
topic anti-angiogenic potential
drug delivery system
expired platelet concentrates
human breast cancer cell line
paclitaxel
platelet-derived extracellular vesicles
Pharmaceutical Science
SDG 3 - Good Health and Well-being
description Funding Information: This research was funded by the Fundação para a Ciência e Tecnologia funded projects PEFPlateletValue (PTDC/BTM-ORG/32187/2017) and EXCELERATE (2022.10467.PTDC), iNOVA4Health-(UIDB/04462/2020 and UIDP/04462/2020), a program financially supported by FCT/Ministério da Ciência, Tecnologia e Ensino Superior, through national funds. The Associate Laboratory LS4FUTURE (LA/P/0087/2020) is also acknowledge. Publisher Copyright: © 2023 by the authors.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-30T22:22:20Z
2023-03
2023-03-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/158552
url http://hdl.handle.net/10362/158552
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1999-4923
PURE: 72698440
https://doi.org/10.3390/pharmaceutics15030953
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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