Detection of Bactrocera oleae (Diptera: Tephritidae) DNA in the gut of the soil species Pseudoophonus rufipes (Coleoptera: Carabidae)

Detalhes bibliográficos
Autor(a) principal: Albertini, Alice
Data de Publicação: 2018
Outros Autores: Santos, Sónia A.P., Martins, Fátima, Pereira, José A., Lino-Neto, Teresa, Petacchi, Ruggero, Baptista, Paula
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/16447
Resumo: Research Article
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spelling Detection of Bactrocera oleae (Diptera: Tephritidae) DNA in the gut of the soil species Pseudoophonus rufipes (Coleoptera: Carabidae)fruit flygut contentolivePCRbiological controlpitfall trappost feeding timeResearch ArticlePest control service provided by natural enemies of Bactrocera oleae, the key pest of the olive tree, is nowadays recognized as fundamental. B. oleae has developed resistance to common insecticides, and negative effects both on consumers’ health and non-target species are the major drawbacks of conventional control strategies. Carabid beetles are potential B. oleae pupae predators, but their predation on field still need to be assessed. We tested adult Pseudoophonus rufipes, a species known to be active in olive orchard when pest pupae are abundant in the soil, in order to detect B. oleae pupae consumption at different post feeding times for both male and female carabids. An already existing protocol was used for detecting B. oleae mtDNA sequences of the cytochrome oxidase subunit I gene in carabids’ gut, and its versatility improved. B. oleae mtDNA was detected up to 20 h after pupa ingestion with a high percentage of success, without significant differences between sexes and pair primers used. Prey DNA extraction was tested from both dissected and non-dissected carabids, obtaining comparable results. The trapping system used to collect carabids for molecular assays and the new elements introduced in the protocol represent cost-effective solutions that may be beneficial for future laboratory trials and, mostly, for the analysis of field-collected predators. Fostering the investigation of soil predators in olive orchard may increase the design of conservation control strategies against B. oleaeINIARepositório da Universidade de LisboaAlbertini, AliceSantos, Sónia A.P.Martins, FátimaPereira, José A.Lino-Neto, TeresaPetacchi, RuggeroBaptista, Paula2018-12-04T14:40:29Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/16447engAlbertini, A.; Santos, S. A. P.; Martins, F.; Pereira, J. A.; Lino-Neto, T.; Petacchi, R.; Baptista, P. (2018). Detection of Bactrocera oleae (Diptera: Tephritidae) DNA in the gut of the soil species Pseudoophonus rufipes (Coleoptera: Carabidae). Spanish Journal of Agricultural Research, Volume 16, Issue 3, e1007https://doi.org/10.5424/sjar/2018163-12860info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:46:15Zoai:www.repository.utl.pt:10400.5/16447Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:01:51.336668Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Detection of Bactrocera oleae (Diptera: Tephritidae) DNA in the gut of the soil species Pseudoophonus rufipes (Coleoptera: Carabidae)
title Detection of Bactrocera oleae (Diptera: Tephritidae) DNA in the gut of the soil species Pseudoophonus rufipes (Coleoptera: Carabidae)
spellingShingle Detection of Bactrocera oleae (Diptera: Tephritidae) DNA in the gut of the soil species Pseudoophonus rufipes (Coleoptera: Carabidae)
Albertini, Alice
fruit fly
gut content
olive
PCR
biological control
pitfall trap
post feeding time
title_short Detection of Bactrocera oleae (Diptera: Tephritidae) DNA in the gut of the soil species Pseudoophonus rufipes (Coleoptera: Carabidae)
title_full Detection of Bactrocera oleae (Diptera: Tephritidae) DNA in the gut of the soil species Pseudoophonus rufipes (Coleoptera: Carabidae)
title_fullStr Detection of Bactrocera oleae (Diptera: Tephritidae) DNA in the gut of the soil species Pseudoophonus rufipes (Coleoptera: Carabidae)
title_full_unstemmed Detection of Bactrocera oleae (Diptera: Tephritidae) DNA in the gut of the soil species Pseudoophonus rufipes (Coleoptera: Carabidae)
title_sort Detection of Bactrocera oleae (Diptera: Tephritidae) DNA in the gut of the soil species Pseudoophonus rufipes (Coleoptera: Carabidae)
author Albertini, Alice
author_facet Albertini, Alice
Santos, Sónia A.P.
Martins, Fátima
Pereira, José A.
Lino-Neto, Teresa
Petacchi, Ruggero
Baptista, Paula
author_role author
author2 Santos, Sónia A.P.
Martins, Fátima
Pereira, José A.
Lino-Neto, Teresa
Petacchi, Ruggero
Baptista, Paula
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Albertini, Alice
Santos, Sónia A.P.
Martins, Fátima
Pereira, José A.
Lino-Neto, Teresa
Petacchi, Ruggero
Baptista, Paula
dc.subject.por.fl_str_mv fruit fly
gut content
olive
PCR
biological control
pitfall trap
post feeding time
topic fruit fly
gut content
olive
PCR
biological control
pitfall trap
post feeding time
description Research Article
publishDate 2018
dc.date.none.fl_str_mv 2018-12-04T14:40:29Z
2018
2018-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/16447
url http://hdl.handle.net/10400.5/16447
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Albertini, A.; Santos, S. A. P.; Martins, F.; Pereira, J. A.; Lino-Neto, T.; Petacchi, R.; Baptista, P. (2018). Detection of Bactrocera oleae (Diptera: Tephritidae) DNA in the gut of the soil species Pseudoophonus rufipes (Coleoptera: Carabidae). Spanish Journal of Agricultural Research, Volume 16, Issue 3, e1007
https://doi.org/10.5424/sjar/2018163-12860
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv INIA
publisher.none.fl_str_mv INIA
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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