Effect of exercise training on lymphocyte subpopulations in chemically and hormonally induced prostate cancer: flow cytometry analysis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/32609 |
Resumo: | Introduction: Long-term and regular exercise training is suggested to have an immunomodulatory effect, protecting against several diseases. This work aimed to analyse the effect of exercise training on peripheral lymphocyte subpopulations in a model of prostate cancer (PCa) chemically and hormonally induced. Methods: Fifty-five male Wistar Unilever rats of 4 weeks of age were randomly divided into four experimental groups as follow: control sedentary group (SED+CONT; n=10), control exercised group (EX+CONT; n=10), induced sedentary group (SED+PCa; n=15) and induced exercised group (EX+PCa; n=20). Prostate lesions were induced through the sequential administration of flutamide (50 mg/kg, TCI Chemicals, USA), testosterone propionate (100 mg/kg, TCI Chemicals, Portland, USA) and N-methyl-N-nitrosourea (30 mg/kg, Sigma Chemical, Spain), and subcutaneous implantation of tubes filled with crystalline testosterone (Sigma Chemical, Spain). At eight weeks of age, exercised animals started the training in a treadmill (Treadmill Control LE 8710, USA), 5 days/weeks, for 53 weeks. Animals were sacrificed at 61 weeks of age through an intraperitoneal injection of ketamine (75 mg/kg, Imalgene® 1000, Merial S.A.S., France) and xylazine (10 mg/kg, Rompun® 2%, Bayer Healthcare S.A., Germany), followed by exsanguination by cardiac puncture. Peripheral blood of all animals was collected by intracardiac puncture and transferred into tubes containing EDTA salt as an anticoagulant for flow cytometry analysis. The following conjugated monoclonal antibodies were used: cyCD3-BV421, CD3-FITC, CD25-APC, CD45-BV510, CD127-PE, CD161-FITC, CD4-PE/Cy7, CD45RA-APC/Cy7, OX-82-PE and CD8a-PerCP. The flow cytometry immunophenotyping was performed in a BD FACSCantoTM II cytometer (BD Biosciences, USA) and data were analysed with InfinicytTM, flow cytometry software 1.7 version. The prostate was collected and stained with H&E for histopathological analysis. Statistical analysis was performed using SPSS 25. The differences were considered statistically significant at p<0.05. Results: A higher level of CD161+NK cells were observed in EX+PCa group when compared with SED+PCa group (p<0.05). These results are in accordance with the literature which suggests that exercise training increase NK cells number. Moreover, long-term exercise training increased gama delta Tcells/CD3 ratio and decreased Treg/NK ratio in PCa-induced groups (p<0.05). Dysplasia, prostatic intraepithelial neoplasia and microinvasive carcinoma were observed in dorsolateral prostate. Although the difference did not reach the level of statistical significance, the exercise training reduced the frequency of the lesions in induced groups (p>0.05). Conclusion: These results reinforce the beneficial role of exercise in anti-tumour immune response. Additional studies are warranted to better understand these results. |
id |
RCAP_d89f20623cfacef87ff05fa5bd8909f2 |
---|---|
oai_identifier_str |
oai:dspace.uevora.pt:10174/32609 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Effect of exercise training on lymphocyte subpopulations in chemically and hormonally induced prostate cancer: flow cytometry analysisIntroduction: Long-term and regular exercise training is suggested to have an immunomodulatory effect, protecting against several diseases. This work aimed to analyse the effect of exercise training on peripheral lymphocyte subpopulations in a model of prostate cancer (PCa) chemically and hormonally induced. Methods: Fifty-five male Wistar Unilever rats of 4 weeks of age were randomly divided into four experimental groups as follow: control sedentary group (SED+CONT; n=10), control exercised group (EX+CONT; n=10), induced sedentary group (SED+PCa; n=15) and induced exercised group (EX+PCa; n=20). Prostate lesions were induced through the sequential administration of flutamide (50 mg/kg, TCI Chemicals, USA), testosterone propionate (100 mg/kg, TCI Chemicals, Portland, USA) and N-methyl-N-nitrosourea (30 mg/kg, Sigma Chemical, Spain), and subcutaneous implantation of tubes filled with crystalline testosterone (Sigma Chemical, Spain). At eight weeks of age, exercised animals started the training in a treadmill (Treadmill Control LE 8710, USA), 5 days/weeks, for 53 weeks. Animals were sacrificed at 61 weeks of age through an intraperitoneal injection of ketamine (75 mg/kg, Imalgene® 1000, Merial S.A.S., France) and xylazine (10 mg/kg, Rompun® 2%, Bayer Healthcare S.A., Germany), followed by exsanguination by cardiac puncture. Peripheral blood of all animals was collected by intracardiac puncture and transferred into tubes containing EDTA salt as an anticoagulant for flow cytometry analysis. The following conjugated monoclonal antibodies were used: cyCD3-BV421, CD3-FITC, CD25-APC, CD45-BV510, CD127-PE, CD161-FITC, CD4-PE/Cy7, CD45RA-APC/Cy7, OX-82-PE and CD8a-PerCP. The flow cytometry immunophenotyping was performed in a BD FACSCantoTM II cytometer (BD Biosciences, USA) and data were analysed with InfinicytTM, flow cytometry software 1.7 version. The prostate was collected and stained with H&E for histopathological analysis. Statistical analysis was performed using SPSS 25. The differences were considered statistically significant at p<0.05. Results: A higher level of CD161+NK cells were observed in EX+PCa group when compared with SED+PCa group (p<0.05). These results are in accordance with the literature which suggests that exercise training increase NK cells number. Moreover, long-term exercise training increased gama delta Tcells/CD3 ratio and decreased Treg/NK ratio in PCa-induced groups (p<0.05). Dysplasia, prostatic intraepithelial neoplasia and microinvasive carcinoma were observed in dorsolateral prostate. Although the difference did not reach the level of statistical significance, the exercise training reduced the frequency of the lesions in induced groups (p>0.05). Conclusion: These results reinforce the beneficial role of exercise in anti-tumour immune response. Additional studies are warranted to better understand these results.XVII Congress of the Iberian Society of Cytometry2022-10-19T14:07:16Z2022-10-192021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/32609http://hdl.handle.net/10174/32609engNascimento-Gonçalves E, Faustino-Rocha AI, Palmeira C, Duarte JA, Moreira-Gonçalves D, Colaço B, Ferreira R, Oliveira PA. 2021. Effect of exercise training on lymphocyte subpopulations in chemically and hormonally induced prostate cancer: flow cytometry analysis. XVII Congress of the Iberian Society of Cytometry, 14 a 18 de junho.naonaosimndanafaustino@uevora.ptndndndndndnd206Nascimento-Gonçalves, ElisabeteFaustino-Rocha, Ana IsabelPalmeira, CarlosDuarte, José AlbertoMoreira-Gonçalves, DanielColaço, BrunoFerreira, RitaOliveira, Paula Alexandrainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:27:41Zoai:dspace.uevora.pt:10174/32609Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:19:35.654674Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Effect of exercise training on lymphocyte subpopulations in chemically and hormonally induced prostate cancer: flow cytometry analysis |
title |
Effect of exercise training on lymphocyte subpopulations in chemically and hormonally induced prostate cancer: flow cytometry analysis |
spellingShingle |
Effect of exercise training on lymphocyte subpopulations in chemically and hormonally induced prostate cancer: flow cytometry analysis Nascimento-Gonçalves, Elisabete |
title_short |
Effect of exercise training on lymphocyte subpopulations in chemically and hormonally induced prostate cancer: flow cytometry analysis |
title_full |
Effect of exercise training on lymphocyte subpopulations in chemically and hormonally induced prostate cancer: flow cytometry analysis |
title_fullStr |
Effect of exercise training on lymphocyte subpopulations in chemically and hormonally induced prostate cancer: flow cytometry analysis |
title_full_unstemmed |
Effect of exercise training on lymphocyte subpopulations in chemically and hormonally induced prostate cancer: flow cytometry analysis |
title_sort |
Effect of exercise training on lymphocyte subpopulations in chemically and hormonally induced prostate cancer: flow cytometry analysis |
author |
Nascimento-Gonçalves, Elisabete |
author_facet |
Nascimento-Gonçalves, Elisabete Faustino-Rocha, Ana Isabel Palmeira, Carlos Duarte, José Alberto Moreira-Gonçalves, Daniel Colaço, Bruno Ferreira, Rita Oliveira, Paula Alexandra |
author_role |
author |
author2 |
Faustino-Rocha, Ana Isabel Palmeira, Carlos Duarte, José Alberto Moreira-Gonçalves, Daniel Colaço, Bruno Ferreira, Rita Oliveira, Paula Alexandra |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Nascimento-Gonçalves, Elisabete Faustino-Rocha, Ana Isabel Palmeira, Carlos Duarte, José Alberto Moreira-Gonçalves, Daniel Colaço, Bruno Ferreira, Rita Oliveira, Paula Alexandra |
description |
Introduction: Long-term and regular exercise training is suggested to have an immunomodulatory effect, protecting against several diseases. This work aimed to analyse the effect of exercise training on peripheral lymphocyte subpopulations in a model of prostate cancer (PCa) chemically and hormonally induced. Methods: Fifty-five male Wistar Unilever rats of 4 weeks of age were randomly divided into four experimental groups as follow: control sedentary group (SED+CONT; n=10), control exercised group (EX+CONT; n=10), induced sedentary group (SED+PCa; n=15) and induced exercised group (EX+PCa; n=20). Prostate lesions were induced through the sequential administration of flutamide (50 mg/kg, TCI Chemicals, USA), testosterone propionate (100 mg/kg, TCI Chemicals, Portland, USA) and N-methyl-N-nitrosourea (30 mg/kg, Sigma Chemical, Spain), and subcutaneous implantation of tubes filled with crystalline testosterone (Sigma Chemical, Spain). At eight weeks of age, exercised animals started the training in a treadmill (Treadmill Control LE 8710, USA), 5 days/weeks, for 53 weeks. Animals were sacrificed at 61 weeks of age through an intraperitoneal injection of ketamine (75 mg/kg, Imalgene® 1000, Merial S.A.S., France) and xylazine (10 mg/kg, Rompun® 2%, Bayer Healthcare S.A., Germany), followed by exsanguination by cardiac puncture. Peripheral blood of all animals was collected by intracardiac puncture and transferred into tubes containing EDTA salt as an anticoagulant for flow cytometry analysis. The following conjugated monoclonal antibodies were used: cyCD3-BV421, CD3-FITC, CD25-APC, CD45-BV510, CD127-PE, CD161-FITC, CD4-PE/Cy7, CD45RA-APC/Cy7, OX-82-PE and CD8a-PerCP. The flow cytometry immunophenotyping was performed in a BD FACSCantoTM II cytometer (BD Biosciences, USA) and data were analysed with InfinicytTM, flow cytometry software 1.7 version. The prostate was collected and stained with H&E for histopathological analysis. Statistical analysis was performed using SPSS 25. The differences were considered statistically significant at p<0.05. Results: A higher level of CD161+NK cells were observed in EX+PCa group when compared with SED+PCa group (p<0.05). These results are in accordance with the literature which suggests that exercise training increase NK cells number. Moreover, long-term exercise training increased gama delta Tcells/CD3 ratio and decreased Treg/NK ratio in PCa-induced groups (p<0.05). Dysplasia, prostatic intraepithelial neoplasia and microinvasive carcinoma were observed in dorsolateral prostate. Although the difference did not reach the level of statistical significance, the exercise training reduced the frequency of the lesions in induced groups (p>0.05). Conclusion: These results reinforce the beneficial role of exercise in anti-tumour immune response. Additional studies are warranted to better understand these results. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01T00:00:00Z 2022-10-19T14:07:16Z 2022-10-19 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/32609 http://hdl.handle.net/10174/32609 |
url |
http://hdl.handle.net/10174/32609 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Nascimento-Gonçalves E, Faustino-Rocha AI, Palmeira C, Duarte JA, Moreira-Gonçalves D, Colaço B, Ferreira R, Oliveira PA. 2021. Effect of exercise training on lymphocyte subpopulations in chemically and hormonally induced prostate cancer: flow cytometry analysis. XVII Congress of the Iberian Society of Cytometry, 14 a 18 de junho. nao nao sim nd anafaustino@uevora.pt nd nd nd nd nd nd 206 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
XVII Congress of the Iberian Society of Cytometry |
publisher.none.fl_str_mv |
XVII Congress of the Iberian Society of Cytometry |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799136677143248896 |