Photopatterned antibodies for selective cell attachment
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/36451 |
Resumo: | We present a phototriggerable system that allows for the spatiotemporal controlled attachment of selected cell types to a biomaterial using immobilized antibodies that specifically target individual cell phenotypes.o-Nitrobenzyl caged biotin was used to functionalize chitosan membranes and mediate site-specific coupling of streptavidin and biotinylated antibodies after light activation. The ability of this system to capture and immobilize specific cells on a surface was tested using endothelial-specific biotinylated antibodies and nonspecific ones as controls. Homogeneous patterned monolayers of human umbilical vein endothelial cells were obtained on CD31-functionalized surfaces. This is a simple and generic approach that is applicable to other ligands, materials, and cell types and shows the flexibility of caged ligands to trigger and control the interaction between cells and biomaterials. |
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Photopatterned antibodies for selective cell attachmentAntibodiesChitosanPatternSelective cell attachmentScience & TechnologyWe present a phototriggerable system that allows for the spatiotemporal controlled attachment of selected cell types to a biomaterial using immobilized antibodies that specifically target individual cell phenotypes.o-Nitrobenzyl caged biotin was used to functionalize chitosan membranes and mediate site-specific coupling of streptavidin and biotinylated antibodies after light activation. The ability of this system to capture and immobilize specific cells on a surface was tested using endothelial-specific biotinylated antibodies and nonspecific ones as controls. Homogeneous patterned monolayers of human umbilical vein endothelial cells were obtained on CD31-functionalized surfaces. This is a simple and generic approach that is applicable to other ligands, materials, and cell types and shows the flexibility of caged ligands to trigger and control the interaction between cells and biomaterials.We thank Martina Knecht (MPIP) for help with the synthesis of caged biotin and Dr. Ron Unger and Prof. C. J. Kirkpatrick (University Clinic Mainz, RepairLab) for providing HUVECs. C.A.C. acknowledges funding support from the Portuguese Foundation for Science and Technology (FCT) (fellowship SFRH/BD/61390/2009) and from the International Max-Planck Research School in Mainz. The research leading to these results has received funding from the European Union's Seventh Framework Programme (FP7/2007-2013) under grant agreement no. REGPOT-CT2012-316331-POLARIS.American Chemical SocietyUniversidade do MinhoCustódio, Catarina A.Miguel-Arranz, V. SanGropeanu, R. A.Gropeanu, M.Wirkner, M.Reis, R. L.Mano, J. F.Del Campo, A.2014-072014-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/36451engCustódio C. A., San Miguel-Arranz V., Gropeanu R. A., Gropeanu M., Wirkner M., Reis R. L., Mano J. F., del Campo A. Photopatterned Antibodies for Selective Cell Attachment, Langmuir, Vol. 30, Issue 33, pp. 10066–10071, doi:10.1021/la502688h, 20140743-746310.1021/la502688h25076392info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:12:40Zoai:repositorium.sdum.uminho.pt:1822/36451Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:04:36.169857Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Photopatterned antibodies for selective cell attachment |
title |
Photopatterned antibodies for selective cell attachment |
spellingShingle |
Photopatterned antibodies for selective cell attachment Custódio, Catarina A. Antibodies Chitosan Pattern Selective cell attachment Science & Technology |
title_short |
Photopatterned antibodies for selective cell attachment |
title_full |
Photopatterned antibodies for selective cell attachment |
title_fullStr |
Photopatterned antibodies for selective cell attachment |
title_full_unstemmed |
Photopatterned antibodies for selective cell attachment |
title_sort |
Photopatterned antibodies for selective cell attachment |
author |
Custódio, Catarina A. |
author_facet |
Custódio, Catarina A. Miguel-Arranz, V. San Gropeanu, R. A. Gropeanu, M. Wirkner, M. Reis, R. L. Mano, J. F. Del Campo, A. |
author_role |
author |
author2 |
Miguel-Arranz, V. San Gropeanu, R. A. Gropeanu, M. Wirkner, M. Reis, R. L. Mano, J. F. Del Campo, A. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Custódio, Catarina A. Miguel-Arranz, V. San Gropeanu, R. A. Gropeanu, M. Wirkner, M. Reis, R. L. Mano, J. F. Del Campo, A. |
dc.subject.por.fl_str_mv |
Antibodies Chitosan Pattern Selective cell attachment Science & Technology |
topic |
Antibodies Chitosan Pattern Selective cell attachment Science & Technology |
description |
We present a phototriggerable system that allows for the spatiotemporal controlled attachment of selected cell types to a biomaterial using immobilized antibodies that specifically target individual cell phenotypes.o-Nitrobenzyl caged biotin was used to functionalize chitosan membranes and mediate site-specific coupling of streptavidin and biotinylated antibodies after light activation. The ability of this system to capture and immobilize specific cells on a surface was tested using endothelial-specific biotinylated antibodies and nonspecific ones as controls. Homogeneous patterned monolayers of human umbilical vein endothelial cells were obtained on CD31-functionalized surfaces. This is a simple and generic approach that is applicable to other ligands, materials, and cell types and shows the flexibility of caged ligands to trigger and control the interaction between cells and biomaterials. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-07 2014-07-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/36451 |
url |
http://hdl.handle.net/1822/36451 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Custódio C. A., San Miguel-Arranz V., Gropeanu R. A., Gropeanu M., Wirkner M., Reis R. L., Mano J. F., del Campo A. Photopatterned Antibodies for Selective Cell Attachment, Langmuir, Vol. 30, Issue 33, pp. 10066–10071, doi:10.1021/la502688h, 2014 0743-7463 10.1021/la502688h 25076392 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132456027160576 |