Phenolic compounds from Actinidia deliciosa leaves: Caco-2 permeability, enzyme inhibitory activity and cell protein profile studies
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.21/9093 |
Resumo: | Chemical compounds from leaves of fruit-producing trees, a waste from agricultural activity can be isolated and used as a source of natural bioactive chemicals. Boiling water was used as an extractant of bioactive compounds from Actinidia deliciosa leaves and co-extracted fibres were removed with ethanol precipitation. Rutin and quercitrin were the main flavonoids identified and quantified by RP-HPLC-DAD. No cytotoxicity was detected for any of the extracts towards Caco- 2 cell line. A permeation of approx. 14% of extract components through the cells monolayer was determined. The cell protein profile of Caco-2 cells was modified when in the presence of the fibre-free extract and transketolase was the protein over-expressed in the presence of polyphenols. Acetylcholinesterase inhibitory activity was also studied, IC50 of 0.56 mg/mL was obtained with the fibre-free extract. A. deliciosa leaves are a good source of phenolic compounds and, therefore, some advantage may be taken of this agricultural residue, due to their biological activity. |
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Phenolic compounds from Actinidia deliciosa leaves: Caco-2 permeability, enzyme inhibitory activity and cell protein profile studiesActinidia deliciosa leavesRutinQuercitrinAcetylcholinesteraseTransketolasePolyphenols permeationCaco-2 cellsChemical compounds from leaves of fruit-producing trees, a waste from agricultural activity can be isolated and used as a source of natural bioactive chemicals. Boiling water was used as an extractant of bioactive compounds from Actinidia deliciosa leaves and co-extracted fibres were removed with ethanol precipitation. Rutin and quercitrin were the main flavonoids identified and quantified by RP-HPLC-DAD. No cytotoxicity was detected for any of the extracts towards Caco- 2 cell line. A permeation of approx. 14% of extract components through the cells monolayer was determined. The cell protein profile of Caco-2 cells was modified when in the presence of the fibre-free extract and transketolase was the protein over-expressed in the presence of polyphenols. Acetylcholinesterase inhibitory activity was also studied, IC50 of 0.56 mg/mL was obtained with the fibre-free extract. A. deliciosa leaves are a good source of phenolic compounds and, therefore, some advantage may be taken of this agricultural residue, due to their biological activity.ElsevierRCIPLHenriques, JoanaFale, PedroPacheco, RitaFlorêncio, Maria HelenaSerralheiro, Maria Luisa2018-11-27T14:41:14Z2018-102018-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/9093engHENRIQUES, Joana; [et al] – Phenolic compounds from Actinidia deliciosa leaves: Caco-2 permeability, enzyme inhibitory activity and cell protein profile studies. Journal of King Saud University Science. ISSN 1018-3647. Vol. 30, N.º 4 (2018), pp. 513-5181018-3647https://doi.org/10.1016/j.jksus.2017.07.007info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T09:57:23Zoai:repositorio.ipl.pt:10400.21/9093Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:17:44.319064Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Phenolic compounds from Actinidia deliciosa leaves: Caco-2 permeability, enzyme inhibitory activity and cell protein profile studies |
title |
Phenolic compounds from Actinidia deliciosa leaves: Caco-2 permeability, enzyme inhibitory activity and cell protein profile studies |
spellingShingle |
Phenolic compounds from Actinidia deliciosa leaves: Caco-2 permeability, enzyme inhibitory activity and cell protein profile studies Henriques, Joana Actinidia deliciosa leaves Rutin Quercitrin Acetylcholinesterase Transketolase Polyphenols permeation Caco-2 cells |
title_short |
Phenolic compounds from Actinidia deliciosa leaves: Caco-2 permeability, enzyme inhibitory activity and cell protein profile studies |
title_full |
Phenolic compounds from Actinidia deliciosa leaves: Caco-2 permeability, enzyme inhibitory activity and cell protein profile studies |
title_fullStr |
Phenolic compounds from Actinidia deliciosa leaves: Caco-2 permeability, enzyme inhibitory activity and cell protein profile studies |
title_full_unstemmed |
Phenolic compounds from Actinidia deliciosa leaves: Caco-2 permeability, enzyme inhibitory activity and cell protein profile studies |
title_sort |
Phenolic compounds from Actinidia deliciosa leaves: Caco-2 permeability, enzyme inhibitory activity and cell protein profile studies |
author |
Henriques, Joana |
author_facet |
Henriques, Joana Fale, Pedro Pacheco, Rita Florêncio, Maria Helena Serralheiro, Maria Luisa |
author_role |
author |
author2 |
Fale, Pedro Pacheco, Rita Florêncio, Maria Helena Serralheiro, Maria Luisa |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
RCIPL |
dc.contributor.author.fl_str_mv |
Henriques, Joana Fale, Pedro Pacheco, Rita Florêncio, Maria Helena Serralheiro, Maria Luisa |
dc.subject.por.fl_str_mv |
Actinidia deliciosa leaves Rutin Quercitrin Acetylcholinesterase Transketolase Polyphenols permeation Caco-2 cells |
topic |
Actinidia deliciosa leaves Rutin Quercitrin Acetylcholinesterase Transketolase Polyphenols permeation Caco-2 cells |
description |
Chemical compounds from leaves of fruit-producing trees, a waste from agricultural activity can be isolated and used as a source of natural bioactive chemicals. Boiling water was used as an extractant of bioactive compounds from Actinidia deliciosa leaves and co-extracted fibres were removed with ethanol precipitation. Rutin and quercitrin were the main flavonoids identified and quantified by RP-HPLC-DAD. No cytotoxicity was detected for any of the extracts towards Caco- 2 cell line. A permeation of approx. 14% of extract components through the cells monolayer was determined. The cell protein profile of Caco-2 cells was modified when in the presence of the fibre-free extract and transketolase was the protein over-expressed in the presence of polyphenols. Acetylcholinesterase inhibitory activity was also studied, IC50 of 0.56 mg/mL was obtained with the fibre-free extract. A. deliciosa leaves are a good source of phenolic compounds and, therefore, some advantage may be taken of this agricultural residue, due to their biological activity. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-27T14:41:14Z 2018-10 2018-10-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.21/9093 |
url |
http://hdl.handle.net/10400.21/9093 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
HENRIQUES, Joana; [et al] – Phenolic compounds from Actinidia deliciosa leaves: Caco-2 permeability, enzyme inhibitory activity and cell protein profile studies. Journal of King Saud University Science. ISSN 1018-3647. Vol. 30, N.º 4 (2018), pp. 513-518 1018-3647 https://doi.org/10.1016/j.jksus.2017.07.007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133440818282496 |