In vitro anticancer activity and antioxidant properties of essential oils from Populus alba L. and Rosmarinus officinalis L. from South Eastern Anatolia of Turkey

• Autor(a) principal: Gezici S.
• Data de Publicação: 2017
• Outros Autores: Sekeroglu N., Kijjoa A.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: https://hdl.handle.net/10216/120464
• topic: essential oil; thiobarbituric acid reactive substance; A-549 cell line; antineoplastic activity; antioxidant activity; antiproliferative activity; apoptosis; Article; comparative study; controlled study; DPPH radical scavenging assay; drug cytotoxicity; human; human cell; hydrodistillation; IC50; in vitro study; lipid oxidation; lipid peroxidation assay; MCF-7 cell line; mitosis inhibition; NCI-H1299 cell line; Populus; Populus alba; rosemary; Turkey (republic)

Background and Purpose: In recent years, essential oils (EOs) have been reported to possess interesting anti-tumor, anti-mutagenic and anti-carcinogenic activities against various cancer cells. Therefore, we aimed to investigate potential biological activities of EOs from white poplar (Populus alba L., Salicaceae) and rosemary (Rosmarinus officinalis L., Lamiaceae). Material and Methods: EOs from P. alba L. and R. officinalis L. were extracted by hydrodistillation. MTT assay was carried out to determine the potential antiproliferative and cytotoxic properties of the essential oils as well as their corresponding IC50, and the inhibition (%) calculated. Antioxidant activity was determined using 2,2-diphenyl-2-picrylhydrazyl (DPPH) assay, and lipid peroxidation capacity was evaluated using thiobarbituric acid-reactive substances (TBARS) method, and the values were calculated using the standards. Results: The EOs were evaluated for their in vitro cytotoxic, antioxidant and lipid oxidation activities. Regarding cytotoxic activity rosemary essential oil possessed strong inhibition (IC50 = 3.06-7.38 µg/mL) of cell proliferation in comparison to that of P. alba L. (IC50 = 10.53-28.16 µg/mL). Additionally, EO from R. officinalis L. was found to have higher antioxidant and lipid peroxidation capacities with IC50 of 10.08 ± 0.15 and 1.76 ± 0.01, respectively. Conclusion: The results suggest that the EOs of both sources exhibited strong antiproliferative, cytotoxic and potent antioxidant properties and therefore they can have potential applications in the cancer treatment. © 2017, Association of Pharmaceutical Teachers of India. All rights reserved.