Cell-specific regulation of acetylcholinesterase expression under inflammatory conditions

Detalhes bibliográficos
Autor(a) principal: Oliveira, P. de
Data de Publicação: 2012
Outros Autores: Gomes, A. Q., Pacheco, T. R., Almeida, V. Vitorino de, Saldanha, C., Calado, A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/10676
Resumo: © 2012 – IOS Press and the authors. All rights reserved
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spelling Cell-specific regulation of acetylcholinesterase expression under inflammatory conditionsAcetylcholinesteraseInflammationGene expression© 2012 – IOS Press and the authors. All rights reservedAcetylcholine (ACh) has been shown to exert an anti-inflammatory function by down-modulating the expression of pro-inflammatory cytokines. Its availability can be regulated at different levels, namely at its synthesis and degradation steps. Accordingly, the expression of acetylcholinesterase (AChE), the enzyme responsible for ACh hydrolysis, has been observed to be modulated in inflammation. To further address the mechanisms underlying this effect, we aimed here at characterizing AChE expression in distinct cellular types pivotal to the inflammatory response. This study was performed in the human acute leukaemia monocytyc cell line, THP-1, in human monocyte-derived primary macrophages and in human umbilical cord vein endothelial cells (HUVEC). In order to subject these cells to inflammatory conditions, THP-1 and macrophage were treated with lipopolysaccharide (LPS) from E.coli and HUVEC were stimulated with the tumour necrosis factor α (TNF-α). Our results showed that although AChE expression was generally up-regulated at the mRNA level under inflammatory conditions, distinct AChE protein expression profiles were surprisingly observed among the distinct cellular types studied. Altogether, these results argue for the existence of cell specific mechanisms that regulate the expression of acetylcholinesterase in inflammation.Fundação para a Ciência e Tecnologia (FCT), Portugal for funding. P.O. was recipient of the Research Fellowship from FCT with the reference IMM/BI/18-2009IOS PressRepositório da Universidade de LisboaOliveira, P. deGomes, A. Q.Pacheco, T. R.Almeida, V. Vitorino deSaldanha, C.Calado, A.2014-03-05T10:48:04Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/10676engClinical Hemorheology and Microcirculation 51 (2012) 129–1371386-0291http://dx.doi.org/10.3233/CH-2011-1520metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-20T17:14:37Zoai:repositorio.ul.pt:10451/10676Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-20T17:14:37Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cell-specific regulation of acetylcholinesterase expression under inflammatory conditions
title Cell-specific regulation of acetylcholinesterase expression under inflammatory conditions
spellingShingle Cell-specific regulation of acetylcholinesterase expression under inflammatory conditions
Oliveira, P. de
Acetylcholinesterase
Inflammation
Gene expression
title_short Cell-specific regulation of acetylcholinesterase expression under inflammatory conditions
title_full Cell-specific regulation of acetylcholinesterase expression under inflammatory conditions
title_fullStr Cell-specific regulation of acetylcholinesterase expression under inflammatory conditions
title_full_unstemmed Cell-specific regulation of acetylcholinesterase expression under inflammatory conditions
title_sort Cell-specific regulation of acetylcholinesterase expression under inflammatory conditions
author Oliveira, P. de
author_facet Oliveira, P. de
Gomes, A. Q.
Pacheco, T. R.
Almeida, V. Vitorino de
Saldanha, C.
Calado, A.
author_role author
author2 Gomes, A. Q.
Pacheco, T. R.
Almeida, V. Vitorino de
Saldanha, C.
Calado, A.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Oliveira, P. de
Gomes, A. Q.
Pacheco, T. R.
Almeida, V. Vitorino de
Saldanha, C.
Calado, A.
dc.subject.por.fl_str_mv Acetylcholinesterase
Inflammation
Gene expression
topic Acetylcholinesterase
Inflammation
Gene expression
description © 2012 – IOS Press and the authors. All rights reserved
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
2014-03-05T10:48:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/10676
url http://hdl.handle.net/10451/10676
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Clinical Hemorheology and Microcirculation 51 (2012) 129–137
1386-0291
http://dx.doi.org/10.3233/CH-2011-1520
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv IOS Press
publisher.none.fl_str_mv IOS Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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