Simultaneous determination of melatonin and trans-resveratrol in wine by dispersive liquid–liquid microextraction followed by HPLC-FLD
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/7668 |
Resumo: | The discovery of melatonin (Mel) in wines triggered a new interest in the paradigm of health benefits and wine consumption, usually ascribed to trans-resveratrol (trans-RSV). In this context, a dispersive liquid-liquid microextraction for the analysis of Mel and trans-RSV in wines by LC-FLD was developed. A 26-1 factorial design was used to identify the significant variables (p < 0.05) and Central Composite Design was used to achieve the optimal conditions: 300 µL of chloroform (extracting solvent), 1500 µL of acetonitrile (disperser solvent) and 1500 mg of NaCl (ionic strength). Excellent linearity (R2 > 0.9999), repeatability (<3.55%), and accuracy (<7.18%) were obtained using a blank matrix and recoveries (>91.9%) using wines. The method was successfully applied to the analyses of Mel (0.63-7.44 ng mL-1) and trans-RSV (169-2616 ng mL-1) in different wine varieties. Comparison with literature point the overall advantages of the new method. |
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Simultaneous determination of melatonin and trans-resveratrol in wine by dispersive liquid–liquid microextraction followed by HPLC-FLDChromatography, High Pressure LiquidFood AnalysisLiquid Phase MicroextractionMelatoninOsmolar ConcentrationResveratrolSolventsTime FactorsWineComposição dos AlimentosThe discovery of melatonin (Mel) in wines triggered a new interest in the paradigm of health benefits and wine consumption, usually ascribed to trans-resveratrol (trans-RSV). In this context, a dispersive liquid-liquid microextraction for the analysis of Mel and trans-RSV in wines by LC-FLD was developed. A 26-1 factorial design was used to identify the significant variables (p < 0.05) and Central Composite Design was used to achieve the optimal conditions: 300 µL of chloroform (extracting solvent), 1500 µL of acetonitrile (disperser solvent) and 1500 mg of NaCl (ionic strength). Excellent linearity (R2 > 0.9999), repeatability (<3.55%), and accuracy (<7.18%) were obtained using a blank matrix and recoveries (>91.9%) using wines. The method was successfully applied to the analyses of Mel (0.63-7.44 ng mL-1) and trans-RSV (169-2616 ng mL-1) in different wine varieties. Comparison with literature point the overall advantages of the new method.Highlights: Novel approach for simultaneous determination of melatonin and trans-resveratrol; The microextraction procedure is optimized by multivariate optimization strategy; Demonstration of the applicability of the final method in real samples; Novel method has potential for rapid analysis of melatonin and trans-resveratrol.This work was supported by PTDC/SAU-NUT/30322/2017 from FCT/MCTES through national funds.ElsevierRepositório Científico do Instituto Nacional de SaúdeViegas, OlgaEsteves, CristinaRocha, JaneteMelo, ArmindoFerreira, Isabel M.P.L.V.O.2021-04-08T10:45:22Z2021-09-122021-09-12T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/7668engFood Chem. 2021 Mar 1;339:128091. doi: 10.1016/j.foodchem.2020.128091. Epub 2020 Sep 12.0308-814610.1016/j.foodchem.2020.128091info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:08Zoai:repositorio.insa.pt:10400.18/7668Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:18.819912Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Simultaneous determination of melatonin and trans-resveratrol in wine by dispersive liquid–liquid microextraction followed by HPLC-FLD |
title |
Simultaneous determination of melatonin and trans-resveratrol in wine by dispersive liquid–liquid microextraction followed by HPLC-FLD |
spellingShingle |
Simultaneous determination of melatonin and trans-resveratrol in wine by dispersive liquid–liquid microextraction followed by HPLC-FLD Viegas, Olga Chromatography, High Pressure Liquid Food Analysis Liquid Phase Microextraction Melatonin Osmolar Concentration Resveratrol Solvents Time Factors Wine Composição dos Alimentos |
title_short |
Simultaneous determination of melatonin and trans-resveratrol in wine by dispersive liquid–liquid microextraction followed by HPLC-FLD |
title_full |
Simultaneous determination of melatonin and trans-resveratrol in wine by dispersive liquid–liquid microextraction followed by HPLC-FLD |
title_fullStr |
Simultaneous determination of melatonin and trans-resveratrol in wine by dispersive liquid–liquid microextraction followed by HPLC-FLD |
title_full_unstemmed |
Simultaneous determination of melatonin and trans-resveratrol in wine by dispersive liquid–liquid microextraction followed by HPLC-FLD |
title_sort |
Simultaneous determination of melatonin and trans-resveratrol in wine by dispersive liquid–liquid microextraction followed by HPLC-FLD |
author |
Viegas, Olga |
author_facet |
Viegas, Olga Esteves, Cristina Rocha, Janete Melo, Armindo Ferreira, Isabel M.P.L.V.O. |
author_role |
author |
author2 |
Esteves, Cristina Rocha, Janete Melo, Armindo Ferreira, Isabel M.P.L.V.O. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Viegas, Olga Esteves, Cristina Rocha, Janete Melo, Armindo Ferreira, Isabel M.P.L.V.O. |
dc.subject.por.fl_str_mv |
Chromatography, High Pressure Liquid Food Analysis Liquid Phase Microextraction Melatonin Osmolar Concentration Resveratrol Solvents Time Factors Wine Composição dos Alimentos |
topic |
Chromatography, High Pressure Liquid Food Analysis Liquid Phase Microextraction Melatonin Osmolar Concentration Resveratrol Solvents Time Factors Wine Composição dos Alimentos |
description |
The discovery of melatonin (Mel) in wines triggered a new interest in the paradigm of health benefits and wine consumption, usually ascribed to trans-resveratrol (trans-RSV). In this context, a dispersive liquid-liquid microextraction for the analysis of Mel and trans-RSV in wines by LC-FLD was developed. A 26-1 factorial design was used to identify the significant variables (p < 0.05) and Central Composite Design was used to achieve the optimal conditions: 300 µL of chloroform (extracting solvent), 1500 µL of acetonitrile (disperser solvent) and 1500 mg of NaCl (ionic strength). Excellent linearity (R2 > 0.9999), repeatability (<3.55%), and accuracy (<7.18%) were obtained using a blank matrix and recoveries (>91.9%) using wines. The method was successfully applied to the analyses of Mel (0.63-7.44 ng mL-1) and trans-RSV (169-2616 ng mL-1) in different wine varieties. Comparison with literature point the overall advantages of the new method. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-04-08T10:45:22Z 2021-09-12 2021-09-12T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/7668 |
url |
http://hdl.handle.net/10400.18/7668 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Food Chem. 2021 Mar 1;339:128091. doi: 10.1016/j.foodchem.2020.128091. Epub 2020 Sep 12. 0308-8146 10.1016/j.foodchem.2020.128091 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132168227651584 |