Genome-Scale Characterization of Mycobacterium abscessus Complex Isolates from Portugal

Detalhes bibliográficos
Autor(a) principal: Carneiro, Sofia
Data de Publicação: 2023
Outros Autores: Pinto, Miguel, Silva, Sónia, Santos, Andrea, Rodrigues, Irene, Santos, Daniela, Duarte, Sílvia, Vieira, Luís, Gomes, João Paulo, Macedo, Rita
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/164079
Resumo: The acquisition of equipment for whole-genome sequencing used in this study was funded by the HERA (Human and Environmental Risk Assessment) project (Grant/2021/PHF/23776) supported by the European Commission through the European Centre for Disease Prevention and Control (ECDC). This work was also partially funded by the GenomePT project (POCI-01-0145-FEDER-022184) supported by COMPETE 2020 (Operational Programme for Competitiveness and Internationalisation (POCI)), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Operational Programme (CRESC Algarve2020) under the PORTUGAL 2020 Partnership Agreement through the European Regional Development Fund (ERDF). Publisher Copyright: © 2023 by the authors.
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spelling Genome-Scale Characterization of Mycobacterium abscessus Complex Isolates from Portugalantimicrobial resistancegenomic diversityMABCMycobacterium abscessusnontuberculous mycobacteriaPortugalwgMLSTwhole-genome sequencingCatalysisMolecular BiologySpectroscopyComputer Science ApplicationsPhysical and Theoretical ChemistryOrganic ChemistryInorganic ChemistrySDG 2 - Zero HungerThe acquisition of equipment for whole-genome sequencing used in this study was funded by the HERA (Human and Environmental Risk Assessment) project (Grant/2021/PHF/23776) supported by the European Commission through the European Centre for Disease Prevention and Control (ECDC). This work was also partially funded by the GenomePT project (POCI-01-0145-FEDER-022184) supported by COMPETE 2020 (Operational Programme for Competitiveness and Internationalisation (POCI)), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Operational Programme (CRESC Algarve2020) under the PORTUGAL 2020 Partnership Agreement through the European Regional Development Fund (ERDF). Publisher Copyright: © 2023 by the authors.The Mycobacterium abscessus complex (MABC) is an emerging, difficult to treat, multidrug-resistant nontuberculous mycobacteria responsible for a wide spectrum of infections and associated with an increasing number of cases worldwide. Dominant circulating clones (DCCs) of MABC have been genetically identified as groups of strains associated with higher prevalence, higher levels of antimicrobial resistance, and worse clinical outcomes. To date, little is known about the genomic characteristics of MABC species circulating in Portugal. Here, we examined the genetic diversity and antimicrobial resistance profiles of 30 MABC strains isolated between 2014 and 2022 in Portugal. The genetic diversity of circulating MABC strains was assessed through a gene-by-gene approach (wgMLST), allowing their subspecies differentiation and the classification of isolates into DCCs. Antimicrobial resistance profiles were defined using phenotypic, molecular, and genomic approaches. The majority of isolates were resistant to at least two antimicrobials, although a poor correlation between phenotype and genotype data was observed. Portuguese genomes were highly diverse, and data suggest the existence of MABC lineages with potential international circulation or cross-border transmission. This study highlights the genetic diversity and antimicrobial resistance profile of circulating MABC isolates in Portugal while representing the first step towards the implementation of a genomic-based surveillance system for MABC at the Portuguese NIH.DCV - Departamento de Ciências da VidaRUNCarneiro, SofiaPinto, MiguelSilva, SóniaSantos, AndreaRodrigues, IreneSantos, DanielaDuarte, SílviaVieira, LuísGomes, João PauloMacedo, Rita2024-02-23T23:59:26Z2023-10-202023-10-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article16application/pdfhttp://hdl.handle.net/10362/164079eng1661-6596PURE: 83889186https://doi.org/10.3390/ijms242015402info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:50:42Zoai:run.unl.pt:10362/164079Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T04:00:02.273918Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genome-Scale Characterization of Mycobacterium abscessus Complex Isolates from Portugal
title Genome-Scale Characterization of Mycobacterium abscessus Complex Isolates from Portugal
spellingShingle Genome-Scale Characterization of Mycobacterium abscessus Complex Isolates from Portugal
Carneiro, Sofia
antimicrobial resistance
genomic diversity
MABC
Mycobacterium abscessus
nontuberculous mycobacteria
Portugal
wgMLST
whole-genome sequencing
Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry
SDG 2 - Zero Hunger
title_short Genome-Scale Characterization of Mycobacterium abscessus Complex Isolates from Portugal
title_full Genome-Scale Characterization of Mycobacterium abscessus Complex Isolates from Portugal
title_fullStr Genome-Scale Characterization of Mycobacterium abscessus Complex Isolates from Portugal
title_full_unstemmed Genome-Scale Characterization of Mycobacterium abscessus Complex Isolates from Portugal
title_sort Genome-Scale Characterization of Mycobacterium abscessus Complex Isolates from Portugal
author Carneiro, Sofia
author_facet Carneiro, Sofia
Pinto, Miguel
Silva, Sónia
Santos, Andrea
Rodrigues, Irene
Santos, Daniela
Duarte, Sílvia
Vieira, Luís
Gomes, João Paulo
Macedo, Rita
author_role author
author2 Pinto, Miguel
Silva, Sónia
Santos, Andrea
Rodrigues, Irene
Santos, Daniela
Duarte, Sílvia
Vieira, Luís
Gomes, João Paulo
Macedo, Rita
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv DCV - Departamento de Ciências da Vida
RUN
dc.contributor.author.fl_str_mv Carneiro, Sofia
Pinto, Miguel
Silva, Sónia
Santos, Andrea
Rodrigues, Irene
Santos, Daniela
Duarte, Sílvia
Vieira, Luís
Gomes, João Paulo
Macedo, Rita
dc.subject.por.fl_str_mv antimicrobial resistance
genomic diversity
MABC
Mycobacterium abscessus
nontuberculous mycobacteria
Portugal
wgMLST
whole-genome sequencing
Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry
SDG 2 - Zero Hunger
topic antimicrobial resistance
genomic diversity
MABC
Mycobacterium abscessus
nontuberculous mycobacteria
Portugal
wgMLST
whole-genome sequencing
Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry
SDG 2 - Zero Hunger
description The acquisition of equipment for whole-genome sequencing used in this study was funded by the HERA (Human and Environmental Risk Assessment) project (Grant/2021/PHF/23776) supported by the European Commission through the European Centre for Disease Prevention and Control (ECDC). This work was also partially funded by the GenomePT project (POCI-01-0145-FEDER-022184) supported by COMPETE 2020 (Operational Programme for Competitiveness and Internationalisation (POCI)), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Operational Programme (CRESC Algarve2020) under the PORTUGAL 2020 Partnership Agreement through the European Regional Development Fund (ERDF). Publisher Copyright: © 2023 by the authors.
publishDate 2023
dc.date.none.fl_str_mv 2023-10-20
2023-10-20T00:00:00Z
2024-02-23T23:59:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/164079
url http://hdl.handle.net/10362/164079
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1661-6596
PURE: 83889186
https://doi.org/10.3390/ijms242015402
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 16
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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