Distribution of mef(A) in Gram-Positive Bacteria from Healthy Portuguese Children

Detalhes bibliográficos
Autor(a) principal: Luna, Vicki A.
Data de Publicação: 2002
Outros Autores: Heiken, Marc, Judge, Kathleen, Ulep, Catherine, Van Kirk, Nicole, Luis, Henrique, Bernardo, Mário, Leitão, José, Roberts, Marilyn C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/33992
Resumo: We screened 615 gram-positive isolates from 150 healthy children for the presence of the erm(A), erm(B), erm(C), erm(F), and mef(A) genes. The mef(A) genes were found in 20 (9%) of the macrolide-resistant isolates, including Enterococcus spp., Staphylococcus spp., and Streptococcus spp. Sixteen of the 19 gram-positive isolates tested carried the other seven open reading frames (ORFs) described in Tn1207.1, a genetic element carrying mef(A) recently described in Streptococcus pneumoniae. The three Staphylococcus spp. did not carry orf1 to orf3. A gram-negative Acinetobacter junii isolate also carried the other seven ORFs described in Tn1207.1. A Staphylococcus aureus isolate, a Streptococcus intermedius isolate, a Streptococcus sp. isolate, and an Enterococcus sp. isolate had their mef(A) genes completely sequenced and showed 100% identity at the DNA and amino acid levels with the mef(A) gene from S. pneumoniae.
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spelling Distribution of mef(A) in Gram-Positive Bacteria from Healthy Portuguese ChildrenWe screened 615 gram-positive isolates from 150 healthy children for the presence of the erm(A), erm(B), erm(C), erm(F), and mef(A) genes. The mef(A) genes were found in 20 (9%) of the macrolide-resistant isolates, including Enterococcus spp., Staphylococcus spp., and Streptococcus spp. Sixteen of the 19 gram-positive isolates tested carried the other seven open reading frames (ORFs) described in Tn1207.1, a genetic element carrying mef(A) recently described in Streptococcus pneumoniae. The three Staphylococcus spp. did not carry orf1 to orf3. A gram-negative Acinetobacter junii isolate also carried the other seven ORFs described in Tn1207.1. A Staphylococcus aureus isolate, a Streptococcus intermedius isolate, a Streptococcus sp. isolate, and an Enterococcus sp. isolate had their mef(A) genes completely sequenced and showed 100% identity at the DNA and amino acid levels with the mef(A) gene from S. pneumoniae.Repositório da Universidade de LisboaLuna, Vicki A.Heiken, MarcJudge, KathleenUlep, CatherineVan Kirk, NicoleLuis, HenriqueBernardo, MárioLeitão, JoséRoberts, Marilyn C.2018-06-20T14:48:41Z20022002-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/33992engLuna VA, Heiken M, Judge K, et al. Distribution of mef(A) in Gram-Positive Bacteria from Healthy Portuguese Children. Antimicrob. Agents Chemother. 2002;46(8):2513-2517.DOI: 10.1128/AAC.46.8.2513–2517.2002info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:29:01Zoai:repositorio.ul.pt:10451/33992Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:48:48.344150Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Distribution of mef(A) in Gram-Positive Bacteria from Healthy Portuguese Children
title Distribution of mef(A) in Gram-Positive Bacteria from Healthy Portuguese Children
spellingShingle Distribution of mef(A) in Gram-Positive Bacteria from Healthy Portuguese Children
Luna, Vicki A.
title_short Distribution of mef(A) in Gram-Positive Bacteria from Healthy Portuguese Children
title_full Distribution of mef(A) in Gram-Positive Bacteria from Healthy Portuguese Children
title_fullStr Distribution of mef(A) in Gram-Positive Bacteria from Healthy Portuguese Children
title_full_unstemmed Distribution of mef(A) in Gram-Positive Bacteria from Healthy Portuguese Children
title_sort Distribution of mef(A) in Gram-Positive Bacteria from Healthy Portuguese Children
author Luna, Vicki A.
author_facet Luna, Vicki A.
Heiken, Marc
Judge, Kathleen
Ulep, Catherine
Van Kirk, Nicole
Luis, Henrique
Bernardo, Mário
Leitão, José
Roberts, Marilyn C.
author_role author
author2 Heiken, Marc
Judge, Kathleen
Ulep, Catherine
Van Kirk, Nicole
Luis, Henrique
Bernardo, Mário
Leitão, José
Roberts, Marilyn C.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Luna, Vicki A.
Heiken, Marc
Judge, Kathleen
Ulep, Catherine
Van Kirk, Nicole
Luis, Henrique
Bernardo, Mário
Leitão, José
Roberts, Marilyn C.
description We screened 615 gram-positive isolates from 150 healthy children for the presence of the erm(A), erm(B), erm(C), erm(F), and mef(A) genes. The mef(A) genes were found in 20 (9%) of the macrolide-resistant isolates, including Enterococcus spp., Staphylococcus spp., and Streptococcus spp. Sixteen of the 19 gram-positive isolates tested carried the other seven open reading frames (ORFs) described in Tn1207.1, a genetic element carrying mef(A) recently described in Streptococcus pneumoniae. The three Staphylococcus spp. did not carry orf1 to orf3. A gram-negative Acinetobacter junii isolate also carried the other seven ORFs described in Tn1207.1. A Staphylococcus aureus isolate, a Streptococcus intermedius isolate, a Streptococcus sp. isolate, and an Enterococcus sp. isolate had their mef(A) genes completely sequenced and showed 100% identity at the DNA and amino acid levels with the mef(A) gene from S. pneumoniae.
publishDate 2002
dc.date.none.fl_str_mv 2002
2002-01-01T00:00:00Z
2018-06-20T14:48:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/33992
url http://hdl.handle.net/10451/33992
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Luna VA, Heiken M, Judge K, et al. Distribution of mef(A) in Gram-Positive Bacteria from Healthy Portuguese Children. Antimicrob. Agents Chemother. 2002;46(8):2513-2517.
DOI: 10.1128/AAC.46.8.2513–2517.2002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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