Serological and Molecular Survey of Leishmania infantum in a Population of Iberian Lynxes (Lynx pardinus)

Detalhes bibliográficos
Autor(a) principal: Lima, Clara M.
Data de Publicação: 2022
Outros Autores: Santarém, Nuno, Neves, Nuno Costa, Sarmento, Pedro, Carrapato, Carlos, de Sousa, Rita, Cardoso, Luís, Cordeiro-da-Silva, Anabela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/8565
Resumo: This article belongs to the Special Issue Advances in Leishmania Research: From Basic Parasite Biology to Disease Control.
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spelling Serological and Molecular Survey of Leishmania infantum in a Population of Iberian Lynxes (Lynx pardinus)Leishmania infantumLeishmaniasisLISAIFATLynx pardinusPCRrK39PortugalInfecções Sistémicas e ZoonosesThis article belongs to the Special Issue Advances in Leishmania Research: From Basic Parasite Biology to Disease Control.Leishmania infantum, the sand fly-transmitted protozoan parasite responsible for leishmaniasis in humans, dogs, and cats, is endemic in the Iberian Peninsula. However, the impact of L. infantum infection on the conservation of the endangered Iberian lynx (Lynx pardinus) is unknown. Herein, we describe for the first time the occurrence of L. infantum infection among a population of reintroduced and wild-born L. pardinus living in the Portuguese Guadiana Valley Park. The presence of infection was addressed by molecular detection of Leishmania kinetoplast DNA (kDNA) in 35 lynxes, with further confirmation of L. infantum species performed by an internally transcribed spacer (ITS)-1 sequencing. Eight blood samples were positive for kDNA, and ITS-1 sequencing confirmed the presence of L. infantum in two of those samples. Exposure to Leishmania was screened in a group of 36 lynxes using an immunofluorescence antibody test (IFAT) and a multi-antigen enzyme-linked immunosorbent assay (ELISA), using SPLA, rK39, and CPX as Leishmania-specific antigens. Four animals presented a positive IFAT at a dilution of 1:40. Eight samples were considered seropositive to all ELISA Leishmania-specific antigens. Agreement between PCR, IFAT, and all ELISA antigens was found for 1 in 27 samples. These results highlight the susceptibility of autochthonous L. pardinus to L. infantum infection. Further investigation is required to assess the impact of L. infantum infection on this wild species conservation.This research was funded by the Parasite Disease Group at i3S, Porto, Portugal, and the Portuguese Foundation for Science and Technology (FCT) under the PhD scholarship number 2020.07306.BD and the project PTDC/CVT-CVT/6798/2020.MDPIRepositório Científico do Instituto Nacional de SaúdeLima, Clara M.Santarém, NunoNeves, Nuno CostaSarmento, PedroCarrapato, Carlosde Sousa, RitaCardoso, LuísCordeiro-da-Silva, Anabela2023-03-20T11:34:25Z2022-12-112022-12-11T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8565engMicroorganisms. 2022 Dec 11;10(12):2447. doi: 10.3390/microorganisms101224472076-260710.3390/microorganisms10122447info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:38Zoai:repositorio.insa.pt:10400.18/8565Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:43:12.454759Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Serological and Molecular Survey of Leishmania infantum in a Population of Iberian Lynxes (Lynx pardinus)
title Serological and Molecular Survey of Leishmania infantum in a Population of Iberian Lynxes (Lynx pardinus)
spellingShingle Serological and Molecular Survey of Leishmania infantum in a Population of Iberian Lynxes (Lynx pardinus)
Lima, Clara M.
Leishmania infantum
Leishmaniasis
LISA
IFAT
Lynx pardinus
PCR
rK39
Portugal
Infecções Sistémicas e Zoonoses
title_short Serological and Molecular Survey of Leishmania infantum in a Population of Iberian Lynxes (Lynx pardinus)
title_full Serological and Molecular Survey of Leishmania infantum in a Population of Iberian Lynxes (Lynx pardinus)
title_fullStr Serological and Molecular Survey of Leishmania infantum in a Population of Iberian Lynxes (Lynx pardinus)
title_full_unstemmed Serological and Molecular Survey of Leishmania infantum in a Population of Iberian Lynxes (Lynx pardinus)
title_sort Serological and Molecular Survey of Leishmania infantum in a Population of Iberian Lynxes (Lynx pardinus)
author Lima, Clara M.
author_facet Lima, Clara M.
Santarém, Nuno
Neves, Nuno Costa
Sarmento, Pedro
Carrapato, Carlos
de Sousa, Rita
Cardoso, Luís
Cordeiro-da-Silva, Anabela
author_role author
author2 Santarém, Nuno
Neves, Nuno Costa
Sarmento, Pedro
Carrapato, Carlos
de Sousa, Rita
Cardoso, Luís
Cordeiro-da-Silva, Anabela
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Lima, Clara M.
Santarém, Nuno
Neves, Nuno Costa
Sarmento, Pedro
Carrapato, Carlos
de Sousa, Rita
Cardoso, Luís
Cordeiro-da-Silva, Anabela
dc.subject.por.fl_str_mv Leishmania infantum
Leishmaniasis
LISA
IFAT
Lynx pardinus
PCR
rK39
Portugal
Infecções Sistémicas e Zoonoses
topic Leishmania infantum
Leishmaniasis
LISA
IFAT
Lynx pardinus
PCR
rK39
Portugal
Infecções Sistémicas e Zoonoses
description This article belongs to the Special Issue Advances in Leishmania Research: From Basic Parasite Biology to Disease Control.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-11
2022-12-11T00:00:00Z
2023-03-20T11:34:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/8565
url http://hdl.handle.net/10400.18/8565
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microorganisms. 2022 Dec 11;10(12):2447. doi: 10.3390/microorganisms10122447
2076-2607
10.3390/microorganisms10122447
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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