An iron-based T1 contrast agent made of iron-phosphate complexes: In vitro and in vivo studies
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/7834 https://doi.org/10.1007/s10334-006-0066-7 |
Resumo: | Abstract A new iron-based T 1 contrast agent consisting of a complex of iron ions coordinated to phosphate and amine ligands (Fe(phos) in short) has been characterized by spectroscopic and magnetic measurements. NMR relaxation studies showed r 1 values to be dependent on the phosphate salt concentration, K2HPO4, present in the medium. r 1 reaches a maximum value of 2.5 mM-1 s-1 for measurements carried out at 7 T and 298 K. 31P MRS, Mssbauer spectroscopy and magnetic measurements of Fe(phos) solutions suggest paramagnetic Fe3+ ions present in the studied iron–phosphate complex. In vitro and in vivo toxicity experiments with C6 cells and CD1 mice, respectively, demonstrated lack of toxicity for Fe(phos) at the highest dose tested in the MRI experiments (12 mM iron for C6 cells and 0.32 mmol iron/kg for mice). Finally, T 1 weighted images of brain tumours in mice have shown positive contrast enhancement of Fe(phos) for tumour afflicted regions in the brain. |
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An iron-based T1 contrast agent made of iron-phosphate complexes: In vitro and in vivo studiesAbstract A new iron-based T 1 contrast agent consisting of a complex of iron ions coordinated to phosphate and amine ligands (Fe(phos) in short) has been characterized by spectroscopic and magnetic measurements. NMR relaxation studies showed r 1 values to be dependent on the phosphate salt concentration, K2HPO4, present in the medium. r 1 reaches a maximum value of 2.5 mM-1 s-1 for measurements carried out at 7 T and 298 K. 31P MRS, Mssbauer spectroscopy and magnetic measurements of Fe(phos) solutions suggest paramagnetic Fe3+ ions present in the studied iron–phosphate complex. In vitro and in vivo toxicity experiments with C6 cells and CD1 mice, respectively, demonstrated lack of toxicity for Fe(phos) at the highest dose tested in the MRI experiments (12 mM iron for C6 cells and 0.32 mmol iron/kg for mice). Finally, T 1 weighted images of brain tumours in mice have shown positive contrast enhancement of Fe(phos) for tumour afflicted regions in the brain.2007info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/7834http://hdl.handle.net/10316/7834https://doi.org/10.1007/s10334-006-0066-7engMagnetic Resonance Materials in Physics, Biology and Medicine. 20:1 (2007) 27-37Rodríguez, ElisendaSimoes, RuiRoig, AnnaMolins, EliesNedelko, NataliyaSlawska-Waniewska, AnnaAime, SilvioArús, CarlesCabañas, MiquelSanfeliu, CoralCerdán, SebastiánGarcía-Martín, Mariainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-08-31T09:45:24Zoai:estudogeral.uc.pt:10316/7834Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:55:33.629585Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
An iron-based T1 contrast agent made of iron-phosphate complexes: In vitro and in vivo studies |
title |
An iron-based T1 contrast agent made of iron-phosphate complexes: In vitro and in vivo studies |
spellingShingle |
An iron-based T1 contrast agent made of iron-phosphate complexes: In vitro and in vivo studies Rodríguez, Elisenda |
title_short |
An iron-based T1 contrast agent made of iron-phosphate complexes: In vitro and in vivo studies |
title_full |
An iron-based T1 contrast agent made of iron-phosphate complexes: In vitro and in vivo studies |
title_fullStr |
An iron-based T1 contrast agent made of iron-phosphate complexes: In vitro and in vivo studies |
title_full_unstemmed |
An iron-based T1 contrast agent made of iron-phosphate complexes: In vitro and in vivo studies |
title_sort |
An iron-based T1 contrast agent made of iron-phosphate complexes: In vitro and in vivo studies |
author |
Rodríguez, Elisenda |
author_facet |
Rodríguez, Elisenda Simoes, Rui Roig, Anna Molins, Elies Nedelko, Nataliya Slawska-Waniewska, Anna Aime, Silvio Arús, Carles Cabañas, Miquel Sanfeliu, Coral Cerdán, Sebastián García-Martín, Maria |
author_role |
author |
author2 |
Simoes, Rui Roig, Anna Molins, Elies Nedelko, Nataliya Slawska-Waniewska, Anna Aime, Silvio Arús, Carles Cabañas, Miquel Sanfeliu, Coral Cerdán, Sebastián García-Martín, Maria |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Rodríguez, Elisenda Simoes, Rui Roig, Anna Molins, Elies Nedelko, Nataliya Slawska-Waniewska, Anna Aime, Silvio Arús, Carles Cabañas, Miquel Sanfeliu, Coral Cerdán, Sebastián García-Martín, Maria |
description |
Abstract A new iron-based T 1 contrast agent consisting of a complex of iron ions coordinated to phosphate and amine ligands (Fe(phos) in short) has been characterized by spectroscopic and magnetic measurements. NMR relaxation studies showed r 1 values to be dependent on the phosphate salt concentration, K2HPO4, present in the medium. r 1 reaches a maximum value of 2.5 mM-1 s-1 for measurements carried out at 7 T and 298 K. 31P MRS, Mssbauer spectroscopy and magnetic measurements of Fe(phos) solutions suggest paramagnetic Fe3+ ions present in the studied iron–phosphate complex. In vitro and in vivo toxicity experiments with C6 cells and CD1 mice, respectively, demonstrated lack of toxicity for Fe(phos) at the highest dose tested in the MRI experiments (12 mM iron for C6 cells and 0.32 mmol iron/kg for mice). Finally, T 1 weighted images of brain tumours in mice have shown positive contrast enhancement of Fe(phos) for tumour afflicted regions in the brain. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/7834 http://hdl.handle.net/10316/7834 https://doi.org/10.1007/s10334-006-0066-7 |
url |
http://hdl.handle.net/10316/7834 https://doi.org/10.1007/s10334-006-0066-7 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Magnetic Resonance Materials in Physics, Biology and Medicine. 20:1 (2007) 27-37 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133842649382912 |