An Insight into Sargassum muticum Cytoprotective Mechanisms against Oxidative Stress on a Human Cell In Vitro Model

Detalhes bibliográficos
Autor(a) principal: Pinteus, Susete
Data de Publicação: 2017
Outros Autores: Lemos, Marco, Silva, Joana, Alves, Celso, Neugebauer, Agnieszka, Freitas, Rafaela, Duarte, Adriana, Pedrosa, Rui
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.8/3639
Resumo: This work was supported through funding provided by the Fundação para a Ciência e Tecnologia (FCT) Strategic Project UID/MAR/04292/2013 granted to MARE and by the Fundação para a Ciência e Tecnologia (FCT) R&D Project “Red2Discovery—The Red macro-algae Sphaerococcus coronopifolius and Asparagopsis armata as targets for marine drug Discovery” (P2020-PTDC/MAR-BIO/6149/2014). This work was also supported by the European Union through EASME Blue Labs project AMALIA—Algae-to-MArket Lab IdeAs (EASME/EMFF/2016/1.2.1.4/03/SI2.750419) and the Integrated Programme of SR&TD “Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate” (reference Centro-01-0145-FEDER-000018), co-funded by Centro 2020 program, Portugal 2020, European Union, through the European Regional Development Fund. Susete Pinteus, Joana Silva and Celso Alves are financial supported by a Grant from FCT—Fundação para a Ciência e Tecnologia (SFRH/BD/96203/2013, SFRH/BD/103255/2014 and SFRH/BD/97764/2013, respectively).
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spelling An Insight into Sargassum muticum Cytoprotective Mechanisms against Oxidative Stress on a Human Cell In Vitro ModelAntioxidantsBiological ProductsCaspase 9Hydrogen PeroxideMCF-7 CellsMembrane Potential, MitochondrialOxidative StressSargassumThis work was supported through funding provided by the Fundação para a Ciência e Tecnologia (FCT) Strategic Project UID/MAR/04292/2013 granted to MARE and by the Fundação para a Ciência e Tecnologia (FCT) R&D Project “Red2Discovery—The Red macro-algae Sphaerococcus coronopifolius and Asparagopsis armata as targets for marine drug Discovery” (P2020-PTDC/MAR-BIO/6149/2014). This work was also supported by the European Union through EASME Blue Labs project AMALIA—Algae-to-MArket Lab IdeAs (EASME/EMFF/2016/1.2.1.4/03/SI2.750419) and the Integrated Programme of SR&TD “Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate” (reference Centro-01-0145-FEDER-000018), co-funded by Centro 2020 program, Portugal 2020, European Union, through the European Regional Development Fund. Susete Pinteus, Joana Silva and Celso Alves are financial supported by a Grant from FCT—Fundação para a Ciência e Tecnologia (SFRH/BD/96203/2013, SFRH/BD/103255/2014 and SFRH/BD/97764/2013, respectively).Sargassum muticum is a brown seaweed with strong potential to be used as a functional food ingredient, mainly due to its antioxidant properties. It is widely used in traditional oriental medicine for the treatment of numerous diseases. Nevertheless, few studies have been conducted to add scientific evidence on its effects as well as on the mechanisms of action involved. In this work, the human cell line MCF-7 was used as an in vitro cellular model to evaluate the capability of Sargassum muticum enriched fractions to protect cells on an oxidative stress condition. The concentration of the bioactive compounds was obtained by vacuum liquid chromatography applied on methanol (M) and 1:1 methanol:dichloromethane (MD) crude extracts, resulting in seven enriched fractions from the M extraction (MF2-MF8), and eight fractions from the MD extraction (MDF1-MDF8). All fractions were tested for cytotoxic properties on MCF-7 cells and the nontoxic ones were tested for their capacity to blunt the damaging effects of hydrogen peroxide-induced oxidative stress. The nontoxic effects were also confirmed in 3T3 fibroblast cells as a nontumor cell line. The antioxidant potential of each fraction, as well as changes in the cell's real-time hydrogen peroxide production, in the mitochondrial membrane potential, and in Caspase-9 activity were evaluated. The results suggest that the protective effects evidenced by S. muticum can be related with the inhibition of hydrogen peroxide production and the inhibition of Caspase-9 activity.MDPIIC-OnlinePinteus, SuseteLemos, MarcoSilva, JoanaAlves, CelsoNeugebauer, AgnieszkaFreitas, RafaelaDuarte, AdrianaPedrosa, Rui2018-11-12T11:35:35Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/3639eng10.3390/md15110353info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:47:36Zoai:iconline.ipleiria.pt:10400.8/3639Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:47:43.156216Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv An Insight into Sargassum muticum Cytoprotective Mechanisms against Oxidative Stress on a Human Cell In Vitro Model
title An Insight into Sargassum muticum Cytoprotective Mechanisms against Oxidative Stress on a Human Cell In Vitro Model
spellingShingle An Insight into Sargassum muticum Cytoprotective Mechanisms against Oxidative Stress on a Human Cell In Vitro Model
Pinteus, Susete
Antioxidants
Biological Products
Caspase 9
Hydrogen Peroxide
MCF-7 Cells
Membrane Potential, Mitochondrial
Oxidative Stress
Sargassum
title_short An Insight into Sargassum muticum Cytoprotective Mechanisms against Oxidative Stress on a Human Cell In Vitro Model
title_full An Insight into Sargassum muticum Cytoprotective Mechanisms against Oxidative Stress on a Human Cell In Vitro Model
title_fullStr An Insight into Sargassum muticum Cytoprotective Mechanisms against Oxidative Stress on a Human Cell In Vitro Model
title_full_unstemmed An Insight into Sargassum muticum Cytoprotective Mechanisms against Oxidative Stress on a Human Cell In Vitro Model
title_sort An Insight into Sargassum muticum Cytoprotective Mechanisms against Oxidative Stress on a Human Cell In Vitro Model
author Pinteus, Susete
author_facet Pinteus, Susete
Lemos, Marco
Silva, Joana
Alves, Celso
Neugebauer, Agnieszka
Freitas, Rafaela
Duarte, Adriana
Pedrosa, Rui
author_role author
author2 Lemos, Marco
Silva, Joana
Alves, Celso
Neugebauer, Agnieszka
Freitas, Rafaela
Duarte, Adriana
Pedrosa, Rui
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv IC-Online
dc.contributor.author.fl_str_mv Pinteus, Susete
Lemos, Marco
Silva, Joana
Alves, Celso
Neugebauer, Agnieszka
Freitas, Rafaela
Duarte, Adriana
Pedrosa, Rui
dc.subject.por.fl_str_mv Antioxidants
Biological Products
Caspase 9
Hydrogen Peroxide
MCF-7 Cells
Membrane Potential, Mitochondrial
Oxidative Stress
Sargassum
topic Antioxidants
Biological Products
Caspase 9
Hydrogen Peroxide
MCF-7 Cells
Membrane Potential, Mitochondrial
Oxidative Stress
Sargassum
description This work was supported through funding provided by the Fundação para a Ciência e Tecnologia (FCT) Strategic Project UID/MAR/04292/2013 granted to MARE and by the Fundação para a Ciência e Tecnologia (FCT) R&D Project “Red2Discovery—The Red macro-algae Sphaerococcus coronopifolius and Asparagopsis armata as targets for marine drug Discovery” (P2020-PTDC/MAR-BIO/6149/2014). This work was also supported by the European Union through EASME Blue Labs project AMALIA—Algae-to-MArket Lab IdeAs (EASME/EMFF/2016/1.2.1.4/03/SI2.750419) and the Integrated Programme of SR&TD “Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate” (reference Centro-01-0145-FEDER-000018), co-funded by Centro 2020 program, Portugal 2020, European Union, through the European Regional Development Fund. Susete Pinteus, Joana Silva and Celso Alves are financial supported by a Grant from FCT—Fundação para a Ciência e Tecnologia (SFRH/BD/96203/2013, SFRH/BD/103255/2014 and SFRH/BD/97764/2013, respectively).
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01T00:00:00Z
2018-11-12T11:35:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.8/3639
url http://hdl.handle.net/10400.8/3639
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.3390/md15110353
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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