Endothelial Cell Culture Under Perfusion On A Polyester-Toner Microfluidic Device

• Autor(a) principal: Urbaczek, Ana Carolina
• Data de Publicação: 2017
• Outros Autores: Leão, Paulo Augusto Gomes Carneiro, De Souza, Fayene Zeferino Ribeiro, Afonso, Ana, Vieira Alberice, Juliana, Cappelini, Luciana Teresa Dias, Carlos, Iracilda Zeppone, Carrilho, Emanuel
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: https://doi.org/10.1038/s41598-017-11043-0
• topic: SCANNING-ELECTRON-MICROSCOPY; MESENCHYMAL STEM-CELLS; ORGANS-ON-CHIPS; SURFACE MODIFICATION; SHEAR-STRESS; ELECTROPHORESIS MICROCHIPS; EXTRACELLULAR-MATRIX; ARTERIAL DIAMETER; POLYMER SURFACES; LASER PRINT; General

This study presents an inexpensive and easy way to produce a microfluidic device that mimics a blood vessel, serving as a start point for cell culture under perfusion, cardiovascular research, and toxicological studies. Endpoint assays (i.e., MTT reduction and NO assays) were used and revealed that the components making up the microchip, which is made of polyester and toner (PT), did not induce cell death or nitric oxide (NO) production. Applying oxygen plasma and fibronectin improved the adhesion and proliferation endothelial cell along the microchannel. As expected, these treatments showed an increase in vascular endothelial growth factor (VEGF-A) concentration profiles, which is correlated with adherence and cell proliferation, thus promoting endothelialization of the device for neovascularization. Regardless the simplicity of the device, our "vein-on-a-chip" mimetic has a potential to serve as a powerful tool for those that demand a rapid microfabrication method in cell biology or organ-on-a-chip research.