Selection and combination of biomarkers to control and prevent the risk of occupational exposure to the mixture of lead, arsenic and manganese

Detalhes bibliográficos
Autor(a) principal: Andrade, Vanda
Data de Publicação: 2014
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/15803
Resumo: Tese de doutoramento, Farmácia (Toxicologia), Universidade de Lisboa, Faculdade de Farmácia, 2014
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spelling Selection and combination of biomarkers to control and prevent the risk of occupational exposure to the mixture of lead, arsenic and manganeseTeses de doutoramento - 2014Tese de doutoramento, Farmácia (Toxicologia), Universidade de Lisboa, Faculdade de Farmácia, 2014Lead (Pb), arsenic (As) and manganese (Mn) are metals with neurotoxic effects that are present in mines as metal mixtures, making miners potentially vulnerable to an increased risk of neurotoxicity. Biomarkers can be crucial tools to detect these disorders in their earlier stages enabling patients to avoid the disease progression. Several biomarkers for Pb, As and Mn have been investigated, yet only for single exposures despite the different metals can interact within the brain. The aim of this work was to identify biomarkers of neurotoxicity which may be used to control and prevent the risk of chronic low levels exposure to the mixture of Pb, As and Mn. After performing an in vivo assay with Wistar rats repeatedly exposed (for 8 days) to Pb, As, Mn or the 3-metal mixture, the co-exposure increased behavioral toxicity and augmented Pb’s deposition in brain more than upon single exposures, with blood Pb levels failing to reflect this alteration, thus, contributing to underestimate the risk associated with Pb-induced damages. Mechanistic insights concerning the consequences of in vivo Pb, As and Mn interactions revealed changes in neurotransmitters and heme precursors’ levels in the brain. Changes of these BMs in peripheral samples suggested their suitability to be used as BMs of exposure and/or neurotoxicity upon exposure to this mixture. A multibiomarker approach based on multiparameter analysis of independent markers was used; the results suggest that the combination of biomarkers is a better tool to predict the type of exposures and the magnitude of neurotoxic effects, better than when used alone. A preliminary human study in a mining population was performed to apply the results obtained in the in vivo model. According to the results, the miners might be exposed to excessive levels of Pb, As and Mn, and the integration of experimentally selected BMs, Pb and Mn blood levels and urinary Pb, As and delta-aminolevulinic acid levels, revealed to be a good tool to identify excessive exposed workers. Exploration of BMs’ integration methodologies seems certainly promising to assess “real-life” scenarios of exposure to chemical mixtures.Santos, Ana Paula Marreilha dos, 1957-Batoréu, Maria Camila Canteiro, 1948-Repositório da Universidade de LisboaAndrade, Vanda2016-09-29T00:30:12Z201420142014-01-01T00:00:00Zdoctoral thesisinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10451/15803TID:101375620enginfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-20T17:17:06Zoai:repositorio.ul.pt:10451/15803Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-20T17:17:06Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Selection and combination of biomarkers to control and prevent the risk of occupational exposure to the mixture of lead, arsenic and manganese
title Selection and combination of biomarkers to control and prevent the risk of occupational exposure to the mixture of lead, arsenic and manganese
spellingShingle Selection and combination of biomarkers to control and prevent the risk of occupational exposure to the mixture of lead, arsenic and manganese
Andrade, Vanda
Teses de doutoramento - 2014
title_short Selection and combination of biomarkers to control and prevent the risk of occupational exposure to the mixture of lead, arsenic and manganese
title_full Selection and combination of biomarkers to control and prevent the risk of occupational exposure to the mixture of lead, arsenic and manganese
title_fullStr Selection and combination of biomarkers to control and prevent the risk of occupational exposure to the mixture of lead, arsenic and manganese
title_full_unstemmed Selection and combination of biomarkers to control and prevent the risk of occupational exposure to the mixture of lead, arsenic and manganese
title_sort Selection and combination of biomarkers to control and prevent the risk of occupational exposure to the mixture of lead, arsenic and manganese
author Andrade, Vanda
author_facet Andrade, Vanda
author_role author
dc.contributor.none.fl_str_mv Santos, Ana Paula Marreilha dos, 1957-
Batoréu, Maria Camila Canteiro, 1948-
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Andrade, Vanda
dc.subject.por.fl_str_mv Teses de doutoramento - 2014
topic Teses de doutoramento - 2014
description Tese de doutoramento, Farmácia (Toxicologia), Universidade de Lisboa, Faculdade de Farmácia, 2014
publishDate 2014
dc.date.none.fl_str_mv 2014
2014
2014-01-01T00:00:00Z
2016-09-29T00:30:12Z
dc.type.driver.fl_str_mv doctoral thesis
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/15803
TID:101375620
url http://hdl.handle.net/10451/15803
identifier_str_mv TID:101375620
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
_version_ 1817548825014829056

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