Monitoring of eicosapentaenoic acid (EPA) production in the microalgae Nannochloropsis oceanica

Detalhes bibliográficos
Autor(a) principal: Sá, Marta
Data de Publicação: 2020
Outros Autores: Ferrer-Ledo, Narcís, Wijffels, Rene, Crespo, João G., Barbosa, María José Somerlate, Galinha, Claudia F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/96405
Resumo: UID/QUI/50006/2019 SFRH/BD/108894/2015
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spelling Monitoring of eicosapentaenoic acid (EPA) production in the microalgae Nannochloropsis oceanicaChemometric modellingEicosapentaenoic acid (EPA)Fluorescence spectroscopyNannochloropsis oceanicaOmega-3 fatty acidAgronomy and Crop ScienceUID/QUI/50006/2019 SFRH/BD/108894/2015With the increase awareness for a healthier food regime and greener environmental processes, microalgae are being looked as a solution for a sustainable production of polyunsaturated fatty acids, such as omega-3 eicosapentaenoic acid (EPA). Nannochloropsis oceanica is an oleaginous microalga, well-known for the ability of EPA accumulation, although higher lipid productivities are still required to make the process competitive. Therefore, three cultivation parameters were tested in the present work (temperature, light cycles and nitrogen supply) in order to study the EPA profile in the polar and neutral fractions of the cells. In addition, an online monitoring tool based on a fluorescence spectroscopy technique was developed with the aim of increasing process knowledge at real time. The results of this work show that nitrogen depletion induces the highest variability in EPA accumulation in the neutral fraction (triacylglycerols). However, to increase the EPA content in the polar fraction a different strategy needs to be implemented, such as decreasing the cultivation temperature or the light available per cell. Chemometric models were developed through PCA (Principal Component Analysis) and PLS (Projection to Latent Structures), using only fluorescence spectra as inputs, enabling the monitoring of EPA in both fractions separately. High explained variance was observed (above 85%) in both fractions, with R2 above 0.81 and slopes above 0.93 for both validation and training data sets. Lower values of cross-validation and prediction errors were observed (between 0.29 and 0.49% g/gDW). The results obtained show that fluorescence spectroscopy is a powerful technique for online monitoring of non-fluorophore molecules, such as EPA, in complex process like microalgae cultivation.LAQV@REQUIMTEDQ - Departamento de QuímicaRUNSá, MartaFerrer-Ledo, NarcísWijffels, ReneCrespo, João G.Barbosa, María José SomerlateGalinha, Claudia F.2022-03-18T01:31:25Z2020-012020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/96405eng2211-9264PURE: 17098904https://doi.org/10.1016/j.algal.2019.101766info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T17:45:00Zoai:run.unl.pt:10362/96405Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T17:45Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Monitoring of eicosapentaenoic acid (EPA) production in the microalgae Nannochloropsis oceanica
title Monitoring of eicosapentaenoic acid (EPA) production in the microalgae Nannochloropsis oceanica
spellingShingle Monitoring of eicosapentaenoic acid (EPA) production in the microalgae Nannochloropsis oceanica
Sá, Marta
Chemometric modelling
Eicosapentaenoic acid (EPA)
Fluorescence spectroscopy
Nannochloropsis oceanica
Omega-3 fatty acid
Agronomy and Crop Science
title_short Monitoring of eicosapentaenoic acid (EPA) production in the microalgae Nannochloropsis oceanica
title_full Monitoring of eicosapentaenoic acid (EPA) production in the microalgae Nannochloropsis oceanica
title_fullStr Monitoring of eicosapentaenoic acid (EPA) production in the microalgae Nannochloropsis oceanica
title_full_unstemmed Monitoring of eicosapentaenoic acid (EPA) production in the microalgae Nannochloropsis oceanica
title_sort Monitoring of eicosapentaenoic acid (EPA) production in the microalgae Nannochloropsis oceanica
author Sá, Marta
author_facet Sá, Marta
Ferrer-Ledo, Narcís
Wijffels, Rene
Crespo, João G.
Barbosa, María José Somerlate
Galinha, Claudia F.
author_role author
author2 Ferrer-Ledo, Narcís
Wijffels, Rene
Crespo, João G.
Barbosa, María José Somerlate
Galinha, Claudia F.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Sá, Marta
Ferrer-Ledo, Narcís
Wijffels, Rene
Crespo, João G.
Barbosa, María José Somerlate
Galinha, Claudia F.
dc.subject.por.fl_str_mv Chemometric modelling
Eicosapentaenoic acid (EPA)
Fluorescence spectroscopy
Nannochloropsis oceanica
Omega-3 fatty acid
Agronomy and Crop Science
topic Chemometric modelling
Eicosapentaenoic acid (EPA)
Fluorescence spectroscopy
Nannochloropsis oceanica
Omega-3 fatty acid
Agronomy and Crop Science
description UID/QUI/50006/2019 SFRH/BD/108894/2015
publishDate 2020
dc.date.none.fl_str_mv 2020-01
2020-01-01T00:00:00Z
2022-03-18T01:31:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/96405
url http://hdl.handle.net/10362/96405
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2211-9264
PURE: 17098904
https://doi.org/10.1016/j.algal.2019.101766
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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