The membrane periodic skeleton is an actomyosin network that regulates axonal diameter and conduction
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/10216/141444 |
Resumo: | Neurons have a membrane periodic skeleton (MPS) composed of actin rings interconnected by spectrin. Here, combining chemical and genetic gain-and loss-of-function assays, we show that in rat hippocampal neurons the MPS is an actomyosin network that controls axonal expansion and contraction. Using super-resolution microscopy, we analyzed the localization of axonal non-muscle myosin II (NMII). We show that active NMII light chains are colocalized with actin rings and organized in a circular periodic manner throughout the axon shaft. In contrast, NMII heavy chains are mostly positioned along the longitudinal axonal axis, being able to crosslink adjacent rings. NMII filaments can play contractile or scaffolding roles determined by their position relative to actin rings and activation state. We also show that MPS destabilization through NMII inactivation affects axonal electrophysiology, increasing action potential conduction velocity. In summary, our findings open new perspectives on axon diameter regulation, with important implications in neuronal biology. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
spelling |
The membrane periodic skeleton is an actomyosin network that regulates axonal diameter and conductionNeurons have a membrane periodic skeleton (MPS) composed of actin rings interconnected by spectrin. Here, combining chemical and genetic gain-and loss-of-function assays, we show that in rat hippocampal neurons the MPS is an actomyosin network that controls axonal expansion and contraction. Using super-resolution microscopy, we analyzed the localization of axonal non-muscle myosin II (NMII). We show that active NMII light chains are colocalized with actin rings and organized in a circular periodic manner throughout the axon shaft. In contrast, NMII heavy chains are mostly positioned along the longitudinal axonal axis, being able to crosslink adjacent rings. NMII filaments can play contractile or scaffolding roles determined by their position relative to actin rings and activation state. We also show that MPS destabilization through NMII inactivation affects axonal electrophysiology, increasing action potential conduction velocity. In summary, our findings open new perspectives on axon diameter regulation, with important implications in neuronal biology.eLife Sciences Publications20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/141444eng2050-084X10.7554/eLife.55471Costa, ARSousa, SCPinto-Costa, RMateus, JCLopes, CDFCosta, ACRosa, DMachado, DPajuelo, LWang, XZhou, FQPereira, AJSampaio, PRubinstein, BYPinto, IMLampe, MAguiar, PSousa, MMinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T13:12:51Zoai:repositorio-aberto.up.pt:10216/141444Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:36:01.966649Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The membrane periodic skeleton is an actomyosin network that regulates axonal diameter and conduction |
title |
The membrane periodic skeleton is an actomyosin network that regulates axonal diameter and conduction |
spellingShingle |
The membrane periodic skeleton is an actomyosin network that regulates axonal diameter and conduction Costa, AR |
title_short |
The membrane periodic skeleton is an actomyosin network that regulates axonal diameter and conduction |
title_full |
The membrane periodic skeleton is an actomyosin network that regulates axonal diameter and conduction |
title_fullStr |
The membrane periodic skeleton is an actomyosin network that regulates axonal diameter and conduction |
title_full_unstemmed |
The membrane periodic skeleton is an actomyosin network that regulates axonal diameter and conduction |
title_sort |
The membrane periodic skeleton is an actomyosin network that regulates axonal diameter and conduction |
author |
Costa, AR |
author_facet |
Costa, AR Sousa, SC Pinto-Costa, R Mateus, JC Lopes, CDF Costa, AC Rosa, D Machado, D Pajuelo, L Wang, X Zhou, FQ Pereira, AJ Sampaio, P Rubinstein, BY Pinto, IM Lampe, M Aguiar, P Sousa, MM |
author_role |
author |
author2 |
Sousa, SC Pinto-Costa, R Mateus, JC Lopes, CDF Costa, AC Rosa, D Machado, D Pajuelo, L Wang, X Zhou, FQ Pereira, AJ Sampaio, P Rubinstein, BY Pinto, IM Lampe, M Aguiar, P Sousa, MM |
author2_role |
author author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Costa, AR Sousa, SC Pinto-Costa, R Mateus, JC Lopes, CDF Costa, AC Rosa, D Machado, D Pajuelo, L Wang, X Zhou, FQ Pereira, AJ Sampaio, P Rubinstein, BY Pinto, IM Lampe, M Aguiar, P Sousa, MM |
description |
Neurons have a membrane periodic skeleton (MPS) composed of actin rings interconnected by spectrin. Here, combining chemical and genetic gain-and loss-of-function assays, we show that in rat hippocampal neurons the MPS is an actomyosin network that controls axonal expansion and contraction. Using super-resolution microscopy, we analyzed the localization of axonal non-muscle myosin II (NMII). We show that active NMII light chains are colocalized with actin rings and organized in a circular periodic manner throughout the axon shaft. In contrast, NMII heavy chains are mostly positioned along the longitudinal axonal axis, being able to crosslink adjacent rings. NMII filaments can play contractile or scaffolding roles determined by their position relative to actin rings and activation state. We also show that MPS destabilization through NMII inactivation affects axonal electrophysiology, increasing action potential conduction velocity. In summary, our findings open new perspectives on axon diameter regulation, with important implications in neuronal biology. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2020-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/10216/141444 |
url |
https://hdl.handle.net/10216/141444 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2050-084X 10.7554/eLife.55471 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
eLife Sciences Publications |
publisher.none.fl_str_mv |
eLife Sciences Publications |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799135672541380609 |