The effect of high-energy environments on the structure of laccase-polymerized poly(catechol)

Detalhes bibliográficos
Autor(a) principal: Jing Su
Data de Publicação: 2018
Outros Autores: Castro, T., Noro, Jennifer Martins, Fu, Jiajia, Wang, Qiang, Silva, C., Cavaco-Paulo, Artur
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/55181
Resumo: The laccase polymerization of catechol was performed using different reactors namely a water bath (WB), an ultrasonic bath (US) and a high-pressure homogenizer (HPH). The total content of free OH and the MALDI-TOF spectra of polymers obtained demonstrated that reactions are favored in the presence of high-energy environments. Higher conversion yields and polymerization degrees (DP) were obtained after polymerization using US or HPH. Molecular dynamic simulation studies supported these findings by revealing a more open enzyme active site upon environments with high molecular agitation. The higher mass transport generated by US and HPH is the main feature responsible for a higher substrate accessibility to the enzyme which contributed to produce longer polymers.
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spelling The effect of high-energy environments on the structure of laccase-polymerized poly(catechol)CatecholPolymerizationLaccaseUltrasonic bathHigh-pressure homogenizerScience & TechnologyThe laccase polymerization of catechol was performed using different reactors namely a water bath (WB), an ultrasonic bath (US) and a high-pressure homogenizer (HPH). The total content of free OH and the MALDI-TOF spectra of polymers obtained demonstrated that reactions are favored in the presence of high-energy environments. Higher conversion yields and polymerization degrees (DP) were obtained after polymerization using US or HPH. Molecular dynamic simulation studies supported these findings by revealing a more open enzyme active site upon environments with high molecular agitation. The higher mass transport generated by US and HPH is the main feature responsible for a higher substrate accessibility to the enzyme which contributed to produce longer polymers.This study was supported by Chinese Government Scholarship under China Scholarship Council (No. 201606790036) and Chinese Foundation Key projects of governmental cooperation in international scientific and technological innovation (No. 2016 YFE0115700) and by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte. Tarsila Castro thanks the senior position funded by the European Union through the European Regional Development Fund (ERDF) under the Competitiveness Operational Program (COP-A1.1.4-E-2015 nr.30/01.09.2016). Access to computing resources funded by the Project “Search-ON2: Revitalization of HPC infrastructure of UMinho” (NORTE-07-0162-FEDER-000086), cofundedbytheNorth Portugal RegionalOperationalProgramme (ON.2– O Novo Norte), under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF), is also gratefully acknowledged. Jennifer Noro also thanks to FCTFundação para a Ciência e a Tecnologia for funding her scholarship (SFRH/BD/121673/2016).info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoJing SuCastro, T.Noro, Jennifer MartinsFu, JiajiaWang, QiangSilva, C.Cavaco-Paulo, Artur2018-112018-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/55181engJing Su; Castro, T.; Jennifer Noro; Fu, Jiajia; Wang, Qiang; Silva, Carla; Cavaco-Paulo, Artur, The effect of high-energy environments on the structure of laccase-polymerized poly(catechol). Ultrasonics Sonochemistry, 48, 275-280, 20181350-41771350-417710.1016/j.ultsonch.2018.05.03330080551http://www.journals.elsevier.com/ultrasonics-sonochemistry/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:49:21Zoai:repositorium.sdum.uminho.pt:1822/55181Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:47:47.813749Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The effect of high-energy environments on the structure of laccase-polymerized poly(catechol)
title The effect of high-energy environments on the structure of laccase-polymerized poly(catechol)
spellingShingle The effect of high-energy environments on the structure of laccase-polymerized poly(catechol)
Jing Su
Catechol
Polymerization
Laccase
Ultrasonic bath
High-pressure homogenizer
Science & Technology
title_short The effect of high-energy environments on the structure of laccase-polymerized poly(catechol)
title_full The effect of high-energy environments on the structure of laccase-polymerized poly(catechol)
title_fullStr The effect of high-energy environments on the structure of laccase-polymerized poly(catechol)
title_full_unstemmed The effect of high-energy environments on the structure of laccase-polymerized poly(catechol)
title_sort The effect of high-energy environments on the structure of laccase-polymerized poly(catechol)
author Jing Su
author_facet Jing Su
Castro, T.
Noro, Jennifer Martins
Fu, Jiajia
Wang, Qiang
Silva, C.
Cavaco-Paulo, Artur
author_role author
author2 Castro, T.
Noro, Jennifer Martins
Fu, Jiajia
Wang, Qiang
Silva, C.
Cavaco-Paulo, Artur
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Jing Su
Castro, T.
Noro, Jennifer Martins
Fu, Jiajia
Wang, Qiang
Silva, C.
Cavaco-Paulo, Artur
dc.subject.por.fl_str_mv Catechol
Polymerization
Laccase
Ultrasonic bath
High-pressure homogenizer
Science & Technology
topic Catechol
Polymerization
Laccase
Ultrasonic bath
High-pressure homogenizer
Science & Technology
description The laccase polymerization of catechol was performed using different reactors namely a water bath (WB), an ultrasonic bath (US) and a high-pressure homogenizer (HPH). The total content of free OH and the MALDI-TOF spectra of polymers obtained demonstrated that reactions are favored in the presence of high-energy environments. Higher conversion yields and polymerization degrees (DP) were obtained after polymerization using US or HPH. Molecular dynamic simulation studies supported these findings by revealing a more open enzyme active site upon environments with high molecular agitation. The higher mass transport generated by US and HPH is the main feature responsible for a higher substrate accessibility to the enzyme which contributed to produce longer polymers.
publishDate 2018
dc.date.none.fl_str_mv 2018-11
2018-11-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/55181
url http://hdl.handle.net/1822/55181
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Jing Su; Castro, T.; Jennifer Noro; Fu, Jiajia; Wang, Qiang; Silva, Carla; Cavaco-Paulo, Artur, The effect of high-energy environments on the structure of laccase-polymerized poly(catechol). Ultrasonics Sonochemistry, 48, 275-280, 2018
1350-4177
1350-4177
10.1016/j.ultsonch.2018.05.033
30080551
http://www.journals.elsevier.com/ultrasonics-sonochemistry/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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