Lipid composition is a determinant for human defensin HNP1 selectivity

Detalhes bibliográficos
Autor(a) principal: Gonçalves, Sónia
Data de Publicação: 2012
Outros Autores: Abade, João, Teixeira, Alexandre, Santos, Nuno C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/10764
Resumo: © 2012 Wiley Periodicals, Inc.
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spelling Lipid composition is a determinant for human defensin HNP1 selectivityHuman neutrophil peptideAntimicrobialPeptideErgosterolFluorescenceQuenching© 2012 Wiley Periodicals, Inc.Human neutrophil peptide 1 (HNP1) is a human defensin with antimicrobial activity against different bacteria (both Gram-positive and negative), fungi, and viruses. HNP1 is stored in the cytoplasmic azurophilic granules of neutrophils. To elucidate the mode of action of this antimicrobial peptide, studies based on its lipid selectivity were carried out. Large unilamellar vesicles with different lipid compositions were used as biomembranes model systems (mammal, fungal, and bacterial models). Changes on the intrinsic fluorescence of HNP1 upon membrane binding/insertion show that HNP1 has quite distinct preferences for mammalian and fungal membrane model systems. HNP1 showed low interaction with glucosylceramide rich membranes, but high sterol selectivity: it has a higher partition for ergosterol-containing membranes (as fungal membranes) and lower interaction with cholesterol-containing membranes (as in mammalian cells). These results reveal that lipid selectivity is a determinant step for HNP1 action. Fluorescence quenching data obtained using acrylamide indicate that HNP1 interacts with membranes without a full insertion in the lipid bilayer. Generalized polarization of laurdan indicates a change in membrane fluidity in the presence of HNP1 for POPC membranes but not for ergosterol-enriched membranes.Contract grant sponsor: Fundação para a Ciência e a Tecnologia – Ministério da Educação e Ciência Contract grant number: PTDC/SAU-BEB/099142/2008 Contract grant number: FP7-PEOPLE IRSES (European Union). contract grant acronym: MEMPEPACROSSWileyRepositório da Universidade de LisboaGonçalves, SóniaAbade, JoãoTeixeira, AlexandreSantos, Nuno C.2014-03-21T11:15:58Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/10764engPeptideScience Volume 98 / Number 40006-3525http://dx.doi.org/10.1002/bip.22088metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-20T17:14:43Zoai:repositorio.ul.pt:10451/10764Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-20T17:14:43Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Lipid composition is a determinant for human defensin HNP1 selectivity
title Lipid composition is a determinant for human defensin HNP1 selectivity
spellingShingle Lipid composition is a determinant for human defensin HNP1 selectivity
Gonçalves, Sónia
Human neutrophil peptide
Antimicrobial
Peptide
Ergosterol
Fluorescence
Quenching
title_short Lipid composition is a determinant for human defensin HNP1 selectivity
title_full Lipid composition is a determinant for human defensin HNP1 selectivity
title_fullStr Lipid composition is a determinant for human defensin HNP1 selectivity
title_full_unstemmed Lipid composition is a determinant for human defensin HNP1 selectivity
title_sort Lipid composition is a determinant for human defensin HNP1 selectivity
author Gonçalves, Sónia
author_facet Gonçalves, Sónia
Abade, João
Teixeira, Alexandre
Santos, Nuno C.
author_role author
author2 Abade, João
Teixeira, Alexandre
Santos, Nuno C.
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Gonçalves, Sónia
Abade, João
Teixeira, Alexandre
Santos, Nuno C.
dc.subject.por.fl_str_mv Human neutrophil peptide
Antimicrobial
Peptide
Ergosterol
Fluorescence
Quenching
topic Human neutrophil peptide
Antimicrobial
Peptide
Ergosterol
Fluorescence
Quenching
description © 2012 Wiley Periodicals, Inc.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
2014-03-21T11:15:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/10764
url http://hdl.handle.net/10451/10764
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PeptideScience Volume 98 / Number 4
0006-3525
http://dx.doi.org/10.1002/bip.22088
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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