Diverse Arbuscular Mycorrhizal Fungi (AMF) communities colonize plants inhabiting a constructed wetland for wastewater treatment

Detalhes bibliográficos
Autor(a) principal: Calheiros, Cristina S. C.
Data de Publicação: 2019
Outros Autores: Pereira, Sofia I. A., Franco, Albina R., Castro, Paula M. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/28137
Resumo: Constructed wetlands (CWs) are biological wastewater treatment systems that comprise several components where plants and associated organisms play an important role in water depuration. Microbial studies emphasize bacterial dynamics, whereas studies of arbuscular mycorrhizal fungi (AMF) are scarce and the functional role of AMF in aquatic and wetland plants is poorly understood. The aim of this study was to analyze the AMF communities colonizing the roots of Canna indica, Canna flaccida, and Watsonia borbonica inhabiting a CW treating wastewater of a tourism unit. The dynamics of the AMF communities were evaluated by Denaturing Gradient Gel Electrophoresis (DGGE) of 18S rRNA gene amplification products along cold (C) and hot (H) seasons for three consecutive years. DGGE profiles allowed the estimation of AMF species richness (S), and Shannon-Wienner (H) and Pielou (J) indexes, for the different plant species, showing differences between species and along the years. Excised bands from DGGE were analyzed and identified through sequencing for arbuscular mycorrhiza, revealing the presence of AMF strains closely related to Glomus sp., Rhizophagus sp. and Acaulospora sp. genera. Concomitant water quality analyses showed that the system was effective in organic and nutrient removal during the sampling period. Findings from this study suggest that AMF diversity found in the CW is influenced by the water constituents, season, and plant species.
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spelling Diverse Arbuscular Mycorrhizal Fungi (AMF) communities colonize plants inhabiting a constructed wetland for wastewater treatmentMycorrhizal fungiTourismDomestic wastewaterAMF diversityPhytoremediationConstructed wetlandWater qualityConstructed wetlands (CWs) are biological wastewater treatment systems that comprise several components where plants and associated organisms play an important role in water depuration. Microbial studies emphasize bacterial dynamics, whereas studies of arbuscular mycorrhizal fungi (AMF) are scarce and the functional role of AMF in aquatic and wetland plants is poorly understood. The aim of this study was to analyze the AMF communities colonizing the roots of Canna indica, Canna flaccida, and Watsonia borbonica inhabiting a CW treating wastewater of a tourism unit. The dynamics of the AMF communities were evaluated by Denaturing Gradient Gel Electrophoresis (DGGE) of 18S rRNA gene amplification products along cold (C) and hot (H) seasons for three consecutive years. DGGE profiles allowed the estimation of AMF species richness (S), and Shannon-Wienner (H) and Pielou (J) indexes, for the different plant species, showing differences between species and along the years. Excised bands from DGGE were analyzed and identified through sequencing for arbuscular mycorrhiza, revealing the presence of AMF strains closely related to Glomus sp., Rhizophagus sp. and Acaulospora sp. genera. Concomitant water quality analyses showed that the system was effective in organic and nutrient removal during the sampling period. Findings from this study suggest that AMF diversity found in the CW is influenced by the water constituents, season, and plant species.MDPIVeritati - Repositório Institucional da Universidade Católica PortuguesaCalheiros, Cristina S. C.Pereira, Sofia I. A.Franco, Albina R.Castro, Paula M. L.2019-08-30T12:24:59Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/28137engCalheiros, C. S. C., Pereira, S. I. A., Franco, A. R., & Castro, P. M. L. (2019). Diverse Arbuscular Mycorrhizal Fungi (AMF) communities colonize plants inhabiting a constructed wetland for wastewater treatment. Water, 11(8), 1535. https://doi.org/10.3390/w1108153510.3390/w110815352073-444185070306909000484561500010info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-09-06T12:26:08Zoai:repositorio.ucp.pt:10400.14/28137Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-09-06T12:26:08Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Diverse Arbuscular Mycorrhizal Fungi (AMF) communities colonize plants inhabiting a constructed wetland for wastewater treatment
title Diverse Arbuscular Mycorrhizal Fungi (AMF) communities colonize plants inhabiting a constructed wetland for wastewater treatment
spellingShingle Diverse Arbuscular Mycorrhizal Fungi (AMF) communities colonize plants inhabiting a constructed wetland for wastewater treatment
Calheiros, Cristina S. C.
Mycorrhizal fungi
Tourism
Domestic wastewater
AMF diversity
Phytoremediation
Constructed wetland
Water quality
title_short Diverse Arbuscular Mycorrhizal Fungi (AMF) communities colonize plants inhabiting a constructed wetland for wastewater treatment
title_full Diverse Arbuscular Mycorrhizal Fungi (AMF) communities colonize plants inhabiting a constructed wetland for wastewater treatment
title_fullStr Diverse Arbuscular Mycorrhizal Fungi (AMF) communities colonize plants inhabiting a constructed wetland for wastewater treatment
title_full_unstemmed Diverse Arbuscular Mycorrhizal Fungi (AMF) communities colonize plants inhabiting a constructed wetland for wastewater treatment
title_sort Diverse Arbuscular Mycorrhizal Fungi (AMF) communities colonize plants inhabiting a constructed wetland for wastewater treatment
author Calheiros, Cristina S. C.
author_facet Calheiros, Cristina S. C.
Pereira, Sofia I. A.
Franco, Albina R.
Castro, Paula M. L.
author_role author
author2 Pereira, Sofia I. A.
Franco, Albina R.
Castro, Paula M. L.
author2_role author
author
author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Calheiros, Cristina S. C.
Pereira, Sofia I. A.
Franco, Albina R.
Castro, Paula M. L.
dc.subject.por.fl_str_mv Mycorrhizal fungi
Tourism
Domestic wastewater
AMF diversity
Phytoremediation
Constructed wetland
Water quality
topic Mycorrhizal fungi
Tourism
Domestic wastewater
AMF diversity
Phytoremediation
Constructed wetland
Water quality
description Constructed wetlands (CWs) are biological wastewater treatment systems that comprise several components where plants and associated organisms play an important role in water depuration. Microbial studies emphasize bacterial dynamics, whereas studies of arbuscular mycorrhizal fungi (AMF) are scarce and the functional role of AMF in aquatic and wetland plants is poorly understood. The aim of this study was to analyze the AMF communities colonizing the roots of Canna indica, Canna flaccida, and Watsonia borbonica inhabiting a CW treating wastewater of a tourism unit. The dynamics of the AMF communities were evaluated by Denaturing Gradient Gel Electrophoresis (DGGE) of 18S rRNA gene amplification products along cold (C) and hot (H) seasons for three consecutive years. DGGE profiles allowed the estimation of AMF species richness (S), and Shannon-Wienner (H) and Pielou (J) indexes, for the different plant species, showing differences between species and along the years. Excised bands from DGGE were analyzed and identified through sequencing for arbuscular mycorrhiza, revealing the presence of AMF strains closely related to Glomus sp., Rhizophagus sp. and Acaulospora sp. genera. Concomitant water quality analyses showed that the system was effective in organic and nutrient removal during the sampling period. Findings from this study suggest that AMF diversity found in the CW is influenced by the water constituents, season, and plant species.
publishDate 2019
dc.date.none.fl_str_mv 2019-08-30T12:24:59Z
2019
2019-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.14/28137
url http://hdl.handle.net/10400.14/28137
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Calheiros, C. S. C., Pereira, S. I. A., Franco, A. R., & Castro, P. M. L. (2019). Diverse Arbuscular Mycorrhizal Fungi (AMF) communities colonize plants inhabiting a constructed wetland for wastewater treatment. Water, 11(8), 1535. https://doi.org/10.3390/w11081535
10.3390/w11081535
2073-4441
85070306909
000484561500010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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