Diverse Arbuscular Mycorrhizal Fungi (AMF) communities colonize plants inhabiting a constructed wetland for wastewater treatment
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/28137 |
Resumo: | Constructed wetlands (CWs) are biological wastewater treatment systems that comprise several components where plants and associated organisms play an important role in water depuration. Microbial studies emphasize bacterial dynamics, whereas studies of arbuscular mycorrhizal fungi (AMF) are scarce and the functional role of AMF in aquatic and wetland plants is poorly understood. The aim of this study was to analyze the AMF communities colonizing the roots of Canna indica, Canna flaccida, and Watsonia borbonica inhabiting a CW treating wastewater of a tourism unit. The dynamics of the AMF communities were evaluated by Denaturing Gradient Gel Electrophoresis (DGGE) of 18S rRNA gene amplification products along cold (C) and hot (H) seasons for three consecutive years. DGGE profiles allowed the estimation of AMF species richness (S), and Shannon-Wienner (H) and Pielou (J) indexes, for the different plant species, showing differences between species and along the years. Excised bands from DGGE were analyzed and identified through sequencing for arbuscular mycorrhiza, revealing the presence of AMF strains closely related to Glomus sp., Rhizophagus sp. and Acaulospora sp. genera. Concomitant water quality analyses showed that the system was effective in organic and nutrient removal during the sampling period. Findings from this study suggest that AMF diversity found in the CW is influenced by the water constituents, season, and plant species. |
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Diverse Arbuscular Mycorrhizal Fungi (AMF) communities colonize plants inhabiting a constructed wetland for wastewater treatmentMycorrhizal fungiTourismDomestic wastewaterAMF diversityPhytoremediationConstructed wetlandWater qualityConstructed wetlands (CWs) are biological wastewater treatment systems that comprise several components where plants and associated organisms play an important role in water depuration. Microbial studies emphasize bacterial dynamics, whereas studies of arbuscular mycorrhizal fungi (AMF) are scarce and the functional role of AMF in aquatic and wetland plants is poorly understood. The aim of this study was to analyze the AMF communities colonizing the roots of Canna indica, Canna flaccida, and Watsonia borbonica inhabiting a CW treating wastewater of a tourism unit. The dynamics of the AMF communities were evaluated by Denaturing Gradient Gel Electrophoresis (DGGE) of 18S rRNA gene amplification products along cold (C) and hot (H) seasons for three consecutive years. DGGE profiles allowed the estimation of AMF species richness (S), and Shannon-Wienner (H) and Pielou (J) indexes, for the different plant species, showing differences between species and along the years. Excised bands from DGGE were analyzed and identified through sequencing for arbuscular mycorrhiza, revealing the presence of AMF strains closely related to Glomus sp., Rhizophagus sp. and Acaulospora sp. genera. Concomitant water quality analyses showed that the system was effective in organic and nutrient removal during the sampling period. Findings from this study suggest that AMF diversity found in the CW is influenced by the water constituents, season, and plant species.MDPIVeritati - Repositório Institucional da Universidade Católica PortuguesaCalheiros, Cristina S. C.Pereira, Sofia I. A.Franco, Albina R.Castro, Paula M. L.2019-08-30T12:24:59Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/28137engCalheiros, C. S. C., Pereira, S. I. A., Franco, A. R., & Castro, P. M. L. (2019). Diverse Arbuscular Mycorrhizal Fungi (AMF) communities colonize plants inhabiting a constructed wetland for wastewater treatment. Water, 11(8), 1535. https://doi.org/10.3390/w1108153510.3390/w110815352073-444185070306909000484561500010info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-09-06T12:26:08Zoai:repositorio.ucp.pt:10400.14/28137Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-09-06T12:26:08Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Diverse Arbuscular Mycorrhizal Fungi (AMF) communities colonize plants inhabiting a constructed wetland for wastewater treatment |
title |
Diverse Arbuscular Mycorrhizal Fungi (AMF) communities colonize plants inhabiting a constructed wetland for wastewater treatment |
spellingShingle |
Diverse Arbuscular Mycorrhizal Fungi (AMF) communities colonize plants inhabiting a constructed wetland for wastewater treatment Calheiros, Cristina S. C. Mycorrhizal fungi Tourism Domestic wastewater AMF diversity Phytoremediation Constructed wetland Water quality |
title_short |
Diverse Arbuscular Mycorrhizal Fungi (AMF) communities colonize plants inhabiting a constructed wetland for wastewater treatment |
title_full |
Diverse Arbuscular Mycorrhizal Fungi (AMF) communities colonize plants inhabiting a constructed wetland for wastewater treatment |
title_fullStr |
Diverse Arbuscular Mycorrhizal Fungi (AMF) communities colonize plants inhabiting a constructed wetland for wastewater treatment |
title_full_unstemmed |
Diverse Arbuscular Mycorrhizal Fungi (AMF) communities colonize plants inhabiting a constructed wetland for wastewater treatment |
title_sort |
Diverse Arbuscular Mycorrhizal Fungi (AMF) communities colonize plants inhabiting a constructed wetland for wastewater treatment |
author |
Calheiros, Cristina S. C. |
author_facet |
Calheiros, Cristina S. C. Pereira, Sofia I. A. Franco, Albina R. Castro, Paula M. L. |
author_role |
author |
author2 |
Pereira, Sofia I. A. Franco, Albina R. Castro, Paula M. L. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Calheiros, Cristina S. C. Pereira, Sofia I. A. Franco, Albina R. Castro, Paula M. L. |
dc.subject.por.fl_str_mv |
Mycorrhizal fungi Tourism Domestic wastewater AMF diversity Phytoremediation Constructed wetland Water quality |
topic |
Mycorrhizal fungi Tourism Domestic wastewater AMF diversity Phytoremediation Constructed wetland Water quality |
description |
Constructed wetlands (CWs) are biological wastewater treatment systems that comprise several components where plants and associated organisms play an important role in water depuration. Microbial studies emphasize bacterial dynamics, whereas studies of arbuscular mycorrhizal fungi (AMF) are scarce and the functional role of AMF in aquatic and wetland plants is poorly understood. The aim of this study was to analyze the AMF communities colonizing the roots of Canna indica, Canna flaccida, and Watsonia borbonica inhabiting a CW treating wastewater of a tourism unit. The dynamics of the AMF communities were evaluated by Denaturing Gradient Gel Electrophoresis (DGGE) of 18S rRNA gene amplification products along cold (C) and hot (H) seasons for three consecutive years. DGGE profiles allowed the estimation of AMF species richness (S), and Shannon-Wienner (H) and Pielou (J) indexes, for the different plant species, showing differences between species and along the years. Excised bands from DGGE were analyzed and identified through sequencing for arbuscular mycorrhiza, revealing the presence of AMF strains closely related to Glomus sp., Rhizophagus sp. and Acaulospora sp. genera. Concomitant water quality analyses showed that the system was effective in organic and nutrient removal during the sampling period. Findings from this study suggest that AMF diversity found in the CW is influenced by the water constituents, season, and plant species. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-08-30T12:24:59Z 2019 2019-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/28137 |
url |
http://hdl.handle.net/10400.14/28137 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Calheiros, C. S. C., Pereira, S. I. A., Franco, A. R., & Castro, P. M. L. (2019). Diverse Arbuscular Mycorrhizal Fungi (AMF) communities colonize plants inhabiting a constructed wetland for wastewater treatment. Water, 11(8), 1535. https://doi.org/10.3390/w11081535 10.3390/w11081535 2073-4441 85070306909 000484561500010 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817546935735681024 |