Development of breast cancer spheroids to evaluate cytotoxic response to an anticancer peptide

Bibliographic Details
Main Author: Cavaco, Marco
Publication Date: 2021
Other Authors: Fraga, Patrícia, Valle, Javier, Andreu, David, Castanho, Miguel A. R. B., Neves, Vera
Format: Article
Language: eng
Source: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Download full: http://hdl.handle.net/10451/57439
Summary: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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spelling Development of breast cancer spheroids to evaluate cytotoxic response to an anticancer peptide3D cell cultureAnticancer peptidesBreast cancerCell monolayersPreclinical studiesSpheroids© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).Breast cancer (BC) is the most commonly diagnosed cancer in women and one of the most common causes of cancer-related deaths. Despite intense research efforts, BC treatment still remains challenging. Improved drug development strategies are needed for impactful benefit to patients. Current preclinical studies rely mostly on cell-based screenings, using two-dimensional (2D) cell monolayers that do not mimic in vivo tumors properly. Herein, we explored the development and characterization of three-dimensional (3D) models, named spheroids, of the most aggressive BC subtypes (triple-negative breast cancer-TNBC; and human-epidermal growth receptor-2-HER2+), using the liquid overlay technique with several selected cell lines. In these cell line-derived spheroids, we studied cell density, proliferation, ultrastructure, apoptosis, reactive oxygen species (ROS) production, and cell permeabilization (live/dead). The results showed a formation of compact and homogeneous spheroids on day 7 after seeding 2000 cells/well for MDA-MB-231 and 5000 cells/well for BT-20 and BT-474. Next, we compared the efficacy of a model anticancer peptide (ACP) in cell monolayers and spheroids. Overall, the results demonstrated spheroids to be less sensitive to treatment than cell monolayers, revealing the need for more robust models in drug development.This research was funded by Portuguese Funding Agency, Fundação para a Ciência e a Tecnologia, FCT IP (grants: PD/BD/128281/2017, PTDC/BIA-BQM/5027/2020, and DL 57/2016/CP1451/CT0023); and “la Caixa” Banking Foundation (grant HR17-00409), under the agreement LCF/PR/HR17/5215001.MDPIRepositório da Universidade de LisboaCavaco, MarcoFraga, PatríciaValle, JavierAndreu, DavidCastanho, Miguel A. R. B.Neves, Vera2023-05-17T14:19:59Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/57439engPharmaceutics 2021, 13, 186310.3390/pharmaceutics131118631999-4923info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T17:05:50Zoai:repositorio.ul.pt:10451/57439Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:07:55.191521Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Development of breast cancer spheroids to evaluate cytotoxic response to an anticancer peptide
title Development of breast cancer spheroids to evaluate cytotoxic response to an anticancer peptide
spellingShingle Development of breast cancer spheroids to evaluate cytotoxic response to an anticancer peptide
Cavaco, Marco
3D cell culture
Anticancer peptides
Breast cancer
Cell monolayers
Preclinical studies
Spheroids
title_short Development of breast cancer spheroids to evaluate cytotoxic response to an anticancer peptide
title_full Development of breast cancer spheroids to evaluate cytotoxic response to an anticancer peptide
title_fullStr Development of breast cancer spheroids to evaluate cytotoxic response to an anticancer peptide
title_full_unstemmed Development of breast cancer spheroids to evaluate cytotoxic response to an anticancer peptide
title_sort Development of breast cancer spheroids to evaluate cytotoxic response to an anticancer peptide
author Cavaco, Marco
author_facet Cavaco, Marco
Fraga, Patrícia
Valle, Javier
Andreu, David
Castanho, Miguel A. R. B.
Neves, Vera
author_role author
author2 Fraga, Patrícia
Valle, Javier
Andreu, David
Castanho, Miguel A. R. B.
Neves, Vera
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Cavaco, Marco
Fraga, Patrícia
Valle, Javier
Andreu, David
Castanho, Miguel A. R. B.
Neves, Vera
dc.subject.por.fl_str_mv 3D cell culture
Anticancer peptides
Breast cancer
Cell monolayers
Preclinical studies
Spheroids
topic 3D cell culture
Anticancer peptides
Breast cancer
Cell monolayers
Preclinical studies
Spheroids
description © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01T00:00:00Z
2023-05-17T14:19:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/57439
url http://hdl.handle.net/10451/57439
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pharmaceutics 2021, 13, 1863
10.3390/pharmaceutics13111863
1999-4923
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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