The actin-binding protein α-adducin is required for maintaining axon diameter
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10216/110361 |
Resumo: | The actin-binding protein adducin was recently identified as a component of the neuronal subcortical cytoskeleton. Here, we analyzed mice lacking adducin to uncover the function of this protein in actin rings. a-adducin knockout mice presented progressive axon enlargement in the spinal cord and optic and sciatic nerves, followed by axon degeneration and loss. Using stimulated emission depletion super-resolution microscopy, we show that a periodic subcortical actin cytoskeleton is assembled in every neuron type inspected including retinal ganglion cells and dorsal root ganglia neurons. In neurons devoid of adducin, the actin ring diameter increased, although the inter-ring periodicity was maintained. In vitro, the actin ring diameter adjusted as axons grew, suggesting the lattice is dynamic. Our data support a model in which adducin activity is not essential for actin ring assembly and periodicity but is necessary to control the diameter of both actin rings and axons and actin filament growth within rings. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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The actin-binding protein α-adducin is required for maintaining axon diameterAnimalsAxonal TransportAxons/metabolismCalmodulin-Binding Proteins/metabolismCytoskeleton/metabolismGrowth Cones/metabolismHippocampus/pathologyMice Inbred C57BLMice KnockoutMicrofilament Proteins/metabolismNerve Degeneration/metabolismNerve Degeneration/pathologyThe actin-binding protein adducin was recently identified as a component of the neuronal subcortical cytoskeleton. Here, we analyzed mice lacking adducin to uncover the function of this protein in actin rings. a-adducin knockout mice presented progressive axon enlargement in the spinal cord and optic and sciatic nerves, followed by axon degeneration and loss. Using stimulated emission depletion super-resolution microscopy, we show that a periodic subcortical actin cytoskeleton is assembled in every neuron type inspected including retinal ganglion cells and dorsal root ganglia neurons. In neurons devoid of adducin, the actin ring diameter increased, although the inter-ring periodicity was maintained. In vitro, the actin ring diameter adjusted as axons grew, suggesting the lattice is dynamic. Our data support a model in which adducin activity is not essential for actin ring assembly and periodicity but is necessary to control the diameter of both actin rings and axons and actin filament growth within rings.20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/10216/110361eng2211-124710.1016/j.celrep.2016.03.047Leite, SCSampaio, PSousa, VFNogueira-Rodrigues, JPinto-Costa, RPeters, LLBrites, PSousa, MMinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T15:58:50Zoai:repositorio-aberto.up.pt:10216/110361Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:36:05.513581Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The actin-binding protein α-adducin is required for maintaining axon diameter |
title |
The actin-binding protein α-adducin is required for maintaining axon diameter |
spellingShingle |
The actin-binding protein α-adducin is required for maintaining axon diameter Leite, SC Animals Axonal Transport Axons/metabolism Calmodulin-Binding Proteins/metabolism Cytoskeleton/metabolism Growth Cones/metabolism Hippocampus/pathology Mice Inbred C57BL Mice Knockout Microfilament Proteins/metabolism Nerve Degeneration/metabolism Nerve Degeneration/pathology |
title_short |
The actin-binding protein α-adducin is required for maintaining axon diameter |
title_full |
The actin-binding protein α-adducin is required for maintaining axon diameter |
title_fullStr |
The actin-binding protein α-adducin is required for maintaining axon diameter |
title_full_unstemmed |
The actin-binding protein α-adducin is required for maintaining axon diameter |
title_sort |
The actin-binding protein α-adducin is required for maintaining axon diameter |
author |
Leite, SC |
author_facet |
Leite, SC Sampaio, P Sousa, VF Nogueira-Rodrigues, J Pinto-Costa, R Peters, LL Brites, P Sousa, MM |
author_role |
author |
author2 |
Sampaio, P Sousa, VF Nogueira-Rodrigues, J Pinto-Costa, R Peters, LL Brites, P Sousa, MM |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Leite, SC Sampaio, P Sousa, VF Nogueira-Rodrigues, J Pinto-Costa, R Peters, LL Brites, P Sousa, MM |
dc.subject.por.fl_str_mv |
Animals Axonal Transport Axons/metabolism Calmodulin-Binding Proteins/metabolism Cytoskeleton/metabolism Growth Cones/metabolism Hippocampus/pathology Mice Inbred C57BL Mice Knockout Microfilament Proteins/metabolism Nerve Degeneration/metabolism Nerve Degeneration/pathology |
topic |
Animals Axonal Transport Axons/metabolism Calmodulin-Binding Proteins/metabolism Cytoskeleton/metabolism Growth Cones/metabolism Hippocampus/pathology Mice Inbred C57BL Mice Knockout Microfilament Proteins/metabolism Nerve Degeneration/metabolism Nerve Degeneration/pathology |
description |
The actin-binding protein adducin was recently identified as a component of the neuronal subcortical cytoskeleton. Here, we analyzed mice lacking adducin to uncover the function of this protein in actin rings. a-adducin knockout mice presented progressive axon enlargement in the spinal cord and optic and sciatic nerves, followed by axon degeneration and loss. Using stimulated emission depletion super-resolution microscopy, we show that a periodic subcortical actin cytoskeleton is assembled in every neuron type inspected including retinal ganglion cells and dorsal root ganglia neurons. In neurons devoid of adducin, the actin ring diameter increased, although the inter-ring periodicity was maintained. In vitro, the actin ring diameter adjusted as axons grew, suggesting the lattice is dynamic. Our data support a model in which adducin activity is not essential for actin ring assembly and periodicity but is necessary to control the diameter of both actin rings and axons and actin filament growth within rings. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2016-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10216/110361 |
url |
http://hdl.handle.net/10216/110361 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2211-1247 10.1016/j.celrep.2016.03.047 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136269845921792 |