Solid-phase extraction for gas chromatographic mass spectrometric determination of the veterinary drug xylazine in human blood.
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.6/626 |
Resumo: | This paper presents a method for the determination of xylazine in whole blood using solid-phase extraction and gas chromatography–mass spectrometry. This technique required only 0.5 mL of sample, and protriptyline was used as internal standard (IS). Limits of detection and quantitation (LOQ) were 2 and 10 ng/mL, respectively. The method was found to be linear between the LOQ and 3.50 ìg/mL, with correlation coefficients higher than 0.9922. Precision (intra- and interday) and accuracy were in conformity with the criteria normally accepted in bioanalytical method validation. The analyte was stable in the matrix for at least 18 h at room temperature and for at least three freeze/thaw cycles. Mean recovery, calculated at three concentration levels, was 87%. To the best of our knowledge, this is the first time that solid-phase extraction is used as sample preparation technique for the determination of this compound in biological media. Because of its simplicity and speed when compared to other extraction techniques, the herein described method can be successfully applied in the diagnosis of intoxications by xylazine. |
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7160 |
spelling |
Solid-phase extraction for gas chromatographic mass spectrometric determination of the veterinary drug xylazine in human blood.This paper presents a method for the determination of xylazine in whole blood using solid-phase extraction and gas chromatography–mass spectrometry. This technique required only 0.5 mL of sample, and protriptyline was used as internal standard (IS). Limits of detection and quantitation (LOQ) were 2 and 10 ng/mL, respectively. The method was found to be linear between the LOQ and 3.50 ìg/mL, with correlation coefficients higher than 0.9922. Precision (intra- and interday) and accuracy were in conformity with the criteria normally accepted in bioanalytical method validation. The analyte was stable in the matrix for at least 18 h at room temperature and for at least three freeze/thaw cycles. Mean recovery, calculated at three concentration levels, was 87%. To the best of our knowledge, this is the first time that solid-phase extraction is used as sample preparation technique for the determination of this compound in biological media. Because of its simplicity and speed when compared to other extraction techniques, the herein described method can be successfully applied in the diagnosis of intoxications by xylazine.uBibliorumGallardo, EugeniaBarroso, MárioMargalho, CláudiaDevesa, N.Pimentel, J.Vieira, Duarte Nuno2010-04-28T10:07:30Z20072007-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.6/626enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-15T09:35:55Zoai:ubibliorum.ubi.pt:10400.6/626Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:42:39.210535Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Solid-phase extraction for gas chromatographic mass spectrometric determination of the veterinary drug xylazine in human blood. |
title |
Solid-phase extraction for gas chromatographic mass spectrometric determination of the veterinary drug xylazine in human blood. |
spellingShingle |
Solid-phase extraction for gas chromatographic mass spectrometric determination of the veterinary drug xylazine in human blood. Gallardo, Eugenia |
title_short |
Solid-phase extraction for gas chromatographic mass spectrometric determination of the veterinary drug xylazine in human blood. |
title_full |
Solid-phase extraction for gas chromatographic mass spectrometric determination of the veterinary drug xylazine in human blood. |
title_fullStr |
Solid-phase extraction for gas chromatographic mass spectrometric determination of the veterinary drug xylazine in human blood. |
title_full_unstemmed |
Solid-phase extraction for gas chromatographic mass spectrometric determination of the veterinary drug xylazine in human blood. |
title_sort |
Solid-phase extraction for gas chromatographic mass spectrometric determination of the veterinary drug xylazine in human blood. |
author |
Gallardo, Eugenia |
author_facet |
Gallardo, Eugenia Barroso, Mário Margalho, Cláudia Devesa, N. Pimentel, J. Vieira, Duarte Nuno |
author_role |
author |
author2 |
Barroso, Mário Margalho, Cláudia Devesa, N. Pimentel, J. Vieira, Duarte Nuno |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
uBibliorum |
dc.contributor.author.fl_str_mv |
Gallardo, Eugenia Barroso, Mário Margalho, Cláudia Devesa, N. Pimentel, J. Vieira, Duarte Nuno |
description |
This paper presents a method for the determination of xylazine in whole blood using solid-phase extraction and gas chromatography–mass spectrometry. This technique required only 0.5 mL of sample, and protriptyline was used as internal standard (IS). Limits of detection and quantitation (LOQ) were 2 and 10 ng/mL, respectively. The method was found to be linear between the LOQ and 3.50 ìg/mL, with correlation coefficients higher than 0.9922. Precision (intra- and interday) and accuracy were in conformity with the criteria normally accepted in bioanalytical method validation. The analyte was stable in the matrix for at least 18 h at room temperature and for at least three freeze/thaw cycles. Mean recovery, calculated at three concentration levels, was 87%. To the best of our knowledge, this is the first time that solid-phase extraction is used as sample preparation technique for the determination of this compound in biological media. Because of its simplicity and speed when compared to other extraction techniques, the herein described method can be successfully applied in the diagnosis of intoxications by xylazine. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007 2007-01-01T00:00:00Z 2010-04-28T10:07:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.6/626 |
url |
http://hdl.handle.net/10400.6/626 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799136326879019008 |