Photocrosslinked acemannan-based 3D matrices for in vitro cell culture
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/66351 |
Resumo: | Current biomedical research has highlighted the potential use of medicinal plant derivatives as advanced suitable biomaterials due to their biological properties. Acemannan (ACE) is the main polysaccharide of Aloe vera (AV) leaves with many reported biological properties, including immunomodulatory activity, antibacterial action, wound healing induction, and cytocompatibility. These properties suggest that ACE is an appealing material for the development of 3D structures for biomedical purposes. In this study, ACE was modified by a methacrylation reaction using methacrylic anhydride. Further 3D sponges were developed by processing modified ACE by photocrosslinking using UV irradiation and the freeze-drying technique. Both FTIR and 1H NMR spectroscopy confirmed the effectiveness of the functionalization. SEM and m-CT revealed that the cross-sections of the methacrylated acemannan (MACE) sponges have open macropores and a narrow pore size distribution within them. We hypothesized that the intrinsic features of ACE could enhance tumor growth, mimicking the biological tumor microenvironment. Therefore, gastric cancer cells (AGS cell line) were cultured in contact with these matrices for up to 14 days to evaluate their cytocompatibility as a processed biomaterial. Results obtained by live-dead assay and MTS showed that cells are viable and metabolically active. Furthermore, confocal laser scanning microscopy analysis showed a homogeneous distribution of cells within the sponge. Therefore, the photocrosslinking on acemmann is proposed as an alternative approach that could significantly improve the processability of acemannan, allowing the production of high added-value structures enabling exploitation of its potential biomedical value. |
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Photocrosslinked acemannan-based 3D matrices for in vitro cell cultureAcemannanBiomaterialsBiomedical applicationsPhotocrosslinkingCiências Médicas::Biotecnologia MédicaScience & TechnologyCurrent biomedical research has highlighted the potential use of medicinal plant derivatives as advanced suitable biomaterials due to their biological properties. Acemannan (ACE) is the main polysaccharide of Aloe vera (AV) leaves with many reported biological properties, including immunomodulatory activity, antibacterial action, wound healing induction, and cytocompatibility. These properties suggest that ACE is an appealing material for the development of 3D structures for biomedical purposes. In this study, ACE was modified by a methacrylation reaction using methacrylic anhydride. Further 3D sponges were developed by processing modified ACE by photocrosslinking using UV irradiation and the freeze-drying technique. Both FTIR and 1H NMR spectroscopy confirmed the effectiveness of the functionalization. SEM and m-CT revealed that the cross-sections of the methacrylated acemannan (MACE) sponges have open macropores and a narrow pore size distribution within them. We hypothesized that the intrinsic features of ACE could enhance tumor growth, mimicking the biological tumor microenvironment. Therefore, gastric cancer cells (AGS cell line) were cultured in contact with these matrices for up to 14 days to evaluate their cytocompatibility as a processed biomaterial. Results obtained by live-dead assay and MTS showed that cells are viable and metabolically active. Furthermore, confocal laser scanning microscopy analysis showed a homogeneous distribution of cells within the sponge. Therefore, the photocrosslinking on acemmann is proposed as an alternative approach that could significantly improve the processability of acemannan, allowing the production of high added-value structures enabling exploitation of its potential biomedical value.Portuguese Foundation for Science and Technology – FCT (SFRH/BPD/112140/2015, SFRH/BPD/85790/2012, respectively). This work was also financially supported by the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement no. REGPOT-CT2012-316331-POLARIS. The authors would like to express thanks to Lorand Laboratories (Texas, USA) for the Acemannan (BiAloeÒ) supplyRoyal Society of ChemistryUniversidade do MinhoSilva, S. S.Costa, Diana Pereira SoaresReis, R. L.2019-062019-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/66351engSilva, S. S., da Costa, D. S., & Reis, R. L. (2019). Photocrosslinked acemannan-based 3D matrices for in vitro cell culture. Journal of Materials Chemistry B, 7(26), 4184-41902050-750X2050-751810.1039/c9tb00593ehttps://pubs.rsc.org/en/content/articlehtml/2019/tb/c9tb00593einfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:17:52Zoai:repositorium.sdum.uminho.pt:1822/66351Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:10:34.152274Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Photocrosslinked acemannan-based 3D matrices for in vitro cell culture |
title |
Photocrosslinked acemannan-based 3D matrices for in vitro cell culture |
spellingShingle |
Photocrosslinked acemannan-based 3D matrices for in vitro cell culture Silva, S. S. Acemannan Biomaterials Biomedical applications Photocrosslinking Ciências Médicas::Biotecnologia Médica Science & Technology |
title_short |
Photocrosslinked acemannan-based 3D matrices for in vitro cell culture |
title_full |
Photocrosslinked acemannan-based 3D matrices for in vitro cell culture |
title_fullStr |
Photocrosslinked acemannan-based 3D matrices for in vitro cell culture |
title_full_unstemmed |
Photocrosslinked acemannan-based 3D matrices for in vitro cell culture |
title_sort |
Photocrosslinked acemannan-based 3D matrices for in vitro cell culture |
author |
Silva, S. S. |
author_facet |
Silva, S. S. Costa, Diana Pereira Soares Reis, R. L. |
author_role |
author |
author2 |
Costa, Diana Pereira Soares Reis, R. L. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Silva, S. S. Costa, Diana Pereira Soares Reis, R. L. |
dc.subject.por.fl_str_mv |
Acemannan Biomaterials Biomedical applications Photocrosslinking Ciências Médicas::Biotecnologia Médica Science & Technology |
topic |
Acemannan Biomaterials Biomedical applications Photocrosslinking Ciências Médicas::Biotecnologia Médica Science & Technology |
description |
Current biomedical research has highlighted the potential use of medicinal plant derivatives as advanced suitable biomaterials due to their biological properties. Acemannan (ACE) is the main polysaccharide of Aloe vera (AV) leaves with many reported biological properties, including immunomodulatory activity, antibacterial action, wound healing induction, and cytocompatibility. These properties suggest that ACE is an appealing material for the development of 3D structures for biomedical purposes. In this study, ACE was modified by a methacrylation reaction using methacrylic anhydride. Further 3D sponges were developed by processing modified ACE by photocrosslinking using UV irradiation and the freeze-drying technique. Both FTIR and 1H NMR spectroscopy confirmed the effectiveness of the functionalization. SEM and m-CT revealed that the cross-sections of the methacrylated acemannan (MACE) sponges have open macropores and a narrow pore size distribution within them. We hypothesized that the intrinsic features of ACE could enhance tumor growth, mimicking the biological tumor microenvironment. Therefore, gastric cancer cells (AGS cell line) were cultured in contact with these matrices for up to 14 days to evaluate their cytocompatibility as a processed biomaterial. Results obtained by live-dead assay and MTS showed that cells are viable and metabolically active. Furthermore, confocal laser scanning microscopy analysis showed a homogeneous distribution of cells within the sponge. Therefore, the photocrosslinking on acemmann is proposed as an alternative approach that could significantly improve the processability of acemannan, allowing the production of high added-value structures enabling exploitation of its potential biomedical value. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-06 2019-06-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/66351 |
url |
http://hdl.handle.net/1822/66351 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Silva, S. S., da Costa, D. S., & Reis, R. L. (2019). Photocrosslinked acemannan-based 3D matrices for in vitro cell culture. Journal of Materials Chemistry B, 7(26), 4184-4190 2050-750X 2050-7518 10.1039/c9tb00593e https://pubs.rsc.org/en/content/articlehtml/2019/tb/c9tb00593e |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
publisher.none.fl_str_mv |
Royal Society of Chemistry |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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