EVALUATION OF 3D PRINTABILITY AND DRUG RELEASE OF DRUG-LOADED MICRONEEDLES
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/147066 |
Resumo: | Microneedles (MNs) have powerful drug delivery abilities and have been considered a promising approach to enhancing the skin's permeability. MNs are commonly produced by traditional fabrication methods which may present several disadvantages. Therefore, in this dissertation, a new fabrication approach, named as Digital Light Processing (DLP), was employed to overcome the mentioned disadvantages. This printing technology was used to fabricate drug-loaded spear microneedles for sustained release applications. Biocompatible and biodegradable polymers: polyethylene glycol (PEG) and polyethylene glycol diacrylate (PEGDA) were used as the bio-based photocurable resins and carbamazepine (CBZ) as the model drug. In the MNs final prototype, the base is composed of PEGDA, and the needles are made of PEGDA / PEG loaded with CBZ. To ensure proper adherence to the building platform the structures were printed using 50 s of exposure time for the first layer and the optimized parameters, 3 mW/cm2 of UV intensity for 3 s, for the remaining layers. Whole printed MNs showed to have good printability and the tip angle was the most susceptible parameter to the printing process. Furthermore, all the printed MNs were consistent and presented a smooth surface. Post-printing curing conditions showed that ideal mechanical properties of the MNs base were obtained at 50 ˚C and 45 min. The influence of the total number of needles per design was evaluated and the design with higher needle thickness showed to be stronger as it presented higher fracture forces of 2.5 N/Needle. Both designs showed a good piercing capacity and no needle damage upon application, however, the array with a higher density of needles required a higher piercing force, 27 ± 2 mN/Needle. Lastly, the two designs with higher needle thickness demonstrated an increasing drug release throughout the entire experiment and after 5 h both designs had ~ 3 % of the drug released. Although only a tiny amount of drug was released throughout the printed MNs, these findings are promising and show the ability of DLP MNs to release drugs during extended periods. |
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EVALUATION OF 3D PRINTABILITY AND DRUG RELEASE OF DRUG-LOADED MICRONEEDLES3D PrintingTransdermal Drug ReleaseMicroneedlesDigital Light ProcessingPhotocurable ResinsSustained ReleaseDomínio/Área Científica::Engenharia e Tecnologia::NanotecnologiaMicroneedles (MNs) have powerful drug delivery abilities and have been considered a promising approach to enhancing the skin's permeability. MNs are commonly produced by traditional fabrication methods which may present several disadvantages. Therefore, in this dissertation, a new fabrication approach, named as Digital Light Processing (DLP), was employed to overcome the mentioned disadvantages. This printing technology was used to fabricate drug-loaded spear microneedles for sustained release applications. Biocompatible and biodegradable polymers: polyethylene glycol (PEG) and polyethylene glycol diacrylate (PEGDA) were used as the bio-based photocurable resins and carbamazepine (CBZ) as the model drug. In the MNs final prototype, the base is composed of PEGDA, and the needles are made of PEGDA / PEG loaded with CBZ. To ensure proper adherence to the building platform the structures were printed using 50 s of exposure time for the first layer and the optimized parameters, 3 mW/cm2 of UV intensity for 3 s, for the remaining layers. Whole printed MNs showed to have good printability and the tip angle was the most susceptible parameter to the printing process. Furthermore, all the printed MNs were consistent and presented a smooth surface. Post-printing curing conditions showed that ideal mechanical properties of the MNs base were obtained at 50 ˚C and 45 min. The influence of the total number of needles per design was evaluated and the design with higher needle thickness showed to be stronger as it presented higher fracture forces of 2.5 N/Needle. Both designs showed a good piercing capacity and no needle damage upon application, however, the array with a higher density of needles required a higher piercing force, 27 ± 2 mN/Needle. Lastly, the two designs with higher needle thickness demonstrated an increasing drug release throughout the entire experiment and after 5 h both designs had ~ 3 % of the drug released. Although only a tiny amount of drug was released throughout the printed MNs, these findings are promising and show the ability of DLP MNs to release drugs during extended periods.As micro-agulhas (Microneedles, MNs) têm capacidades de administração de fármacos significativas e têm sido consideradas uma abordagem muito promissora para melhorar a permeabilidade da pele. As MNs são geralmente fabricadas por métodos tradicionais que podem apresentar várias desvantagens. Desta forma, nesta dissertação, uma nova abordagem de fabricação, processamento digital de luz (Digital Light Processing, DLP) foi usada para ultrapassar as desvantagens mencionadas. Esta técnica de impressão foi utilizada para fabricar MNs com fármacos incorporados para libertação sustentada de fármacos. Polímeros biocompatíveis e biodegradáveis: o polietilenoglicol (PEG) e o polietilenoglicol diacrilato (PEGDA) foram utilizados como bioresinas fotocuráveis e a carbamazepina (CBZ) como medicamento modelo. No protótipo final das MNs, a base é composta por PEGDA, e as agulhas são feitas de PEGDA / PEG com CBZ. Para assegurar a adesão adequada à plataforma da impressão, as estruturas foram impressas utilizando 50 s de tempo de exposição para a primeira camada e os parâmetros optimizados obtidos, 3 mW/cm2 de intensidade UV durante 3 s, para as restantes camadas. Todas as MNs impressas demonstraram ter boa capacidade de impressão e o ângulo da agulha foi o parâmetro mais susceptível ao processo de impressão. Além disso, todas as MNs impressas eram consistentes e apresentavam uma superfície lisa. As condições de cura pós-impressão mostraram que as propriedades mecânicas ideais da base de MNs foram obtidas em 50 ˚C e 45 min. A influência do número total de agulhas por design foi avaliada e o design com maior espessura de agulha mostrou ser o mais forte, uma vez que apresentou maiores valores de forças de fratura de 2.5 N/Needle. Ambos os designs demostraram uma boa capacidade de perfuração e nenhum dano da agulha aquando da aplicação, contudo, o conjunto com maior densidade de agulhas exigia uma maior força de perfuração, 27 ± 2 mN/Needle. Por fim, os dois designs com agulhas mais espessas demonstraram uma libertação do fármaco crescente durante toda a experiência e após 5 h os dois designs tinham ~ 3 % do fármaco libertado. Embora apenas uma pequena quantidade de fármaco tenha sido libertada a partir das MNs, estes desenvolvimentos são promissores e demostram a capacidade da utilização de DLP para fabricação de MNs capazes de libertar fármacos durante períodos prolongados.Douroumis, DennisBranquinho, RitaRUNViegas, Beatriz2022-12-062024-09-30T00:00:00Z2022-12-06T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/147066enginfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:28:04Zoai:run.unl.pt:10362/147066Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:52:46.804581Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
EVALUATION OF 3D PRINTABILITY AND DRUG RELEASE OF DRUG-LOADED MICRONEEDLES |
title |
EVALUATION OF 3D PRINTABILITY AND DRUG RELEASE OF DRUG-LOADED MICRONEEDLES |
spellingShingle |
EVALUATION OF 3D PRINTABILITY AND DRUG RELEASE OF DRUG-LOADED MICRONEEDLES Viegas, Beatriz 3D Printing Transdermal Drug Release Microneedles Digital Light Processing Photocurable Resins Sustained Release Domínio/Área Científica::Engenharia e Tecnologia::Nanotecnologia |
title_short |
EVALUATION OF 3D PRINTABILITY AND DRUG RELEASE OF DRUG-LOADED MICRONEEDLES |
title_full |
EVALUATION OF 3D PRINTABILITY AND DRUG RELEASE OF DRUG-LOADED MICRONEEDLES |
title_fullStr |
EVALUATION OF 3D PRINTABILITY AND DRUG RELEASE OF DRUG-LOADED MICRONEEDLES |
title_full_unstemmed |
EVALUATION OF 3D PRINTABILITY AND DRUG RELEASE OF DRUG-LOADED MICRONEEDLES |
title_sort |
EVALUATION OF 3D PRINTABILITY AND DRUG RELEASE OF DRUG-LOADED MICRONEEDLES |
author |
Viegas, Beatriz |
author_facet |
Viegas, Beatriz |
author_role |
author |
dc.contributor.none.fl_str_mv |
Douroumis, Dennis Branquinho, Rita RUN |
dc.contributor.author.fl_str_mv |
Viegas, Beatriz |
dc.subject.por.fl_str_mv |
3D Printing Transdermal Drug Release Microneedles Digital Light Processing Photocurable Resins Sustained Release Domínio/Área Científica::Engenharia e Tecnologia::Nanotecnologia |
topic |
3D Printing Transdermal Drug Release Microneedles Digital Light Processing Photocurable Resins Sustained Release Domínio/Área Científica::Engenharia e Tecnologia::Nanotecnologia |
description |
Microneedles (MNs) have powerful drug delivery abilities and have been considered a promising approach to enhancing the skin's permeability. MNs are commonly produced by traditional fabrication methods which may present several disadvantages. Therefore, in this dissertation, a new fabrication approach, named as Digital Light Processing (DLP), was employed to overcome the mentioned disadvantages. This printing technology was used to fabricate drug-loaded spear microneedles for sustained release applications. Biocompatible and biodegradable polymers: polyethylene glycol (PEG) and polyethylene glycol diacrylate (PEGDA) were used as the bio-based photocurable resins and carbamazepine (CBZ) as the model drug. In the MNs final prototype, the base is composed of PEGDA, and the needles are made of PEGDA / PEG loaded with CBZ. To ensure proper adherence to the building platform the structures were printed using 50 s of exposure time for the first layer and the optimized parameters, 3 mW/cm2 of UV intensity for 3 s, for the remaining layers. Whole printed MNs showed to have good printability and the tip angle was the most susceptible parameter to the printing process. Furthermore, all the printed MNs were consistent and presented a smooth surface. Post-printing curing conditions showed that ideal mechanical properties of the MNs base were obtained at 50 ˚C and 45 min. The influence of the total number of needles per design was evaluated and the design with higher needle thickness showed to be stronger as it presented higher fracture forces of 2.5 N/Needle. Both designs showed a good piercing capacity and no needle damage upon application, however, the array with a higher density of needles required a higher piercing force, 27 ± 2 mN/Needle. Lastly, the two designs with higher needle thickness demonstrated an increasing drug release throughout the entire experiment and after 5 h both designs had ~ 3 % of the drug released. Although only a tiny amount of drug was released throughout the printed MNs, these findings are promising and show the ability of DLP MNs to release drugs during extended periods. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-06 2022-12-06T00:00:00Z 2024-09-30T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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http://hdl.handle.net/10362/147066 |
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eng |
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eng |
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