Distinct roles for dynein light intermediate chains in neurogenesis, migration, and terminal somal translocation

Detalhes bibliográficos
Autor(a) principal: Gonçalves, João Carlos
Data de Publicação: 2019
Outros Autores: Dantas, Tiago J., Vallee, Richard B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/67235
Resumo: Cytoplasmic dynein participates in multiple aspects of neocortical development. These include neural progenitor proliferation, morphogenesis, and neuronal migration. The cytoplasmic dynein light intermediate chains (LICs) 1 and 2 are cargo-binding subunits, though their relative roles are not well understood. Here, we used in utero electroporation of shRNAs or LIC functional domains to determine the relative contributions of the two LICs in the developing rat brain. We find that LIC1, through BicD2, is required for apical nuclear migration in neural progenitors. In newborn neurons, we observe specific roles for LIC1 in the multipolar to bipolar transition and glial-guided neuronal migration. In contrast, LIC2 contributes to a novel dynein role in the little-studied mode of migration, terminal somal translocation. Together, our results provide novel insight into the LICs' unique functions during brain development and dynein regulation overall.
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spelling Distinct roles for dynein light intermediate chains in neurogenesis, migration, and terminal somal translocationAnimalsBrainCytoplasmic DyneinsElectroporationNeural Stem CellsRNA, Small InterferingRatsRats, Sprague-DawleyCell MovementNeurogenesisScience & TechnologyCytoplasmic dynein participates in multiple aspects of neocortical development. These include neural progenitor proliferation, morphogenesis, and neuronal migration. The cytoplasmic dynein light intermediate chains (LICs) 1 and 2 are cargo-binding subunits, though their relative roles are not well understood. Here, we used in utero electroporation of shRNAs or LIC functional domains to determine the relative contributions of the two LICs in the developing rat brain. We find that LIC1, through BicD2, is required for apical nuclear migration in neural progenitors. In newborn neurons, we observe specific roles for LIC1 in the multipolar to bipolar transition and glial-guided neuronal migration. In contrast, LIC2 contributes to a novel dynein role in the little-studied mode of migration, terminal somal translocation. Together, our results provide novel insight into the LICs' unique functions during brain development and dynein regulation overall.This project was supported by National Institutes of Health grants HD40182 and GM105536 to R.B. Vallee and the Fundação para Ciência e a Tecnologia MDPhD Scholarship PD/BD/113766/2015 to J.C. Gonçalves. During the final year, T.J. Dan-tas was supported by the Porto Neurosciences and Neurologic Disease Research Initiative at Instituto de Investigação e Inovação em Saúde (Norte-01-0145-FED ER-000008)Rockefeller University PressUniversidade do MinhoGonçalves, João CarlosDantas, Tiago J.Vallee, Richard B.20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/67235eng0021-95251540-814010.1083/jcb.20180611230674581https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400572/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:37:16Zoai:repositorium.sdum.uminho.pt:1822/67235Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:33:32.500952Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Distinct roles for dynein light intermediate chains in neurogenesis, migration, and terminal somal translocation
title Distinct roles for dynein light intermediate chains in neurogenesis, migration, and terminal somal translocation
spellingShingle Distinct roles for dynein light intermediate chains in neurogenesis, migration, and terminal somal translocation
Gonçalves, João Carlos
Animals
Brain
Cytoplasmic Dyneins
Electroporation
Neural Stem Cells
RNA, Small Interfering
Rats
Rats, Sprague-Dawley
Cell Movement
Neurogenesis
Science & Technology
title_short Distinct roles for dynein light intermediate chains in neurogenesis, migration, and terminal somal translocation
title_full Distinct roles for dynein light intermediate chains in neurogenesis, migration, and terminal somal translocation
title_fullStr Distinct roles for dynein light intermediate chains in neurogenesis, migration, and terminal somal translocation
title_full_unstemmed Distinct roles for dynein light intermediate chains in neurogenesis, migration, and terminal somal translocation
title_sort Distinct roles for dynein light intermediate chains in neurogenesis, migration, and terminal somal translocation
author Gonçalves, João Carlos
author_facet Gonçalves, João Carlos
Dantas, Tiago J.
Vallee, Richard B.
author_role author
author2 Dantas, Tiago J.
Vallee, Richard B.
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Gonçalves, João Carlos
Dantas, Tiago J.
Vallee, Richard B.
dc.subject.por.fl_str_mv Animals
Brain
Cytoplasmic Dyneins
Electroporation
Neural Stem Cells
RNA, Small Interfering
Rats
Rats, Sprague-Dawley
Cell Movement
Neurogenesis
Science & Technology
topic Animals
Brain
Cytoplasmic Dyneins
Electroporation
Neural Stem Cells
RNA, Small Interfering
Rats
Rats, Sprague-Dawley
Cell Movement
Neurogenesis
Science & Technology
description Cytoplasmic dynein participates in multiple aspects of neocortical development. These include neural progenitor proliferation, morphogenesis, and neuronal migration. The cytoplasmic dynein light intermediate chains (LICs) 1 and 2 are cargo-binding subunits, though their relative roles are not well understood. Here, we used in utero electroporation of shRNAs or LIC functional domains to determine the relative contributions of the two LICs in the developing rat brain. We find that LIC1, through BicD2, is required for apical nuclear migration in neural progenitors. In newborn neurons, we observe specific roles for LIC1 in the multipolar to bipolar transition and glial-guided neuronal migration. In contrast, LIC2 contributes to a novel dynein role in the little-studied mode of migration, terminal somal translocation. Together, our results provide novel insight into the LICs' unique functions during brain development and dynein regulation overall.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/67235
url http://hdl.handle.net/1822/67235
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0021-9525
1540-8140
10.1083/jcb.201806112
30674581
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400572/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Rockefeller University Press
publisher.none.fl_str_mv Rockefeller University Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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