ROS/RNS Balancing, Aerobic Fermentation Regulation and Cell Cycle Control – a Complex Early Trait (‘CoV-MAC-TED’) for Combating SARS-CoV-2-Induced Cell Reprogramming
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/32855 https://doi.org/10.3389/fimmu.2021.673692 |
Resumo: | In a perspective entitled ‘From plant survival under severe stress to anti-viral human defense’ we raised and justified the hypothesis that transcript level profiles of justified target genes established from in vitro somatic embryogenesis (SE) induction in plants as a reference compared to virus-induced profiles can identify differential virus signatures that link to harmful reprogramming. A standard profile of selected genes named ‘ReprogVirus’ was proposed for in vitro-scanning of early virus-induced reprogramming in critical primary infected cells/tissues as target trait. For data collection, the ‘ReprogVirus platform’ was initiated. This initiative aims to identify in a common effort across scientific boundaries critical virus footprints from diverse virus origins and variants as a basis for anti-viral strategy design. This approach is open for validation and extension. In the present study, we initiated validation by experimental transcriptome data available in public domain combined with advancing plant wet lab research. We compared plant-adapted transcriptomes according to ‘RegroVirus’ complemented by alternative oxidase (AOX) genes during de novo programming under SE-inducing conditions with in vitro corona virus-induced transcriptome profiles. This approach enabled identifying a major complex trait for early de novo programming during SARS-CoV-2 infection, called ‘CoV-MAC-TED’. It consists of unbalanced ROS/RNS levels, which are connected to increased aerobic fermentation that links to alpha-tubulin-based cell restructuration and progression of cell cycle. We conclude that anti-viral/anti-SARS-CoV-2 strategies need to rigorously target ‘CoV-MAC-TED’ in primary infected nose and mouth cells through prophylactic and very early therapeutic strategies. We also discuss potential strategies in the view of the beneficial role of AOX for resilient behavior in plants. Furthermore, following the general observation that ROS/RNS equilibration/redox homeostasis is of utmost importance at the very beginning of viral infection, we highlight that ‘de-stressing’ disease and social handling should be seen as essential part of anti-viral/anti-SARS-CoV-2 strategies. |
id |
RCAP_e139f2ce158501763e850c4c1f14f6eb |
---|---|
oai_identifier_str |
oai:dspace.uevora.pt:10174/32855 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
ROS/RNS Balancing, Aerobic Fermentation Regulation and Cell Cycle Control – a Complex Early Trait (‘CoV-MAC-TED’) for Combating SARS-CoV-2-Induced Cell ReprogrammingSARS-CoV-2redox biologyalternative oxidasetubulinmTORmelatoninrepurposing drugsIn a perspective entitled ‘From plant survival under severe stress to anti-viral human defense’ we raised and justified the hypothesis that transcript level profiles of justified target genes established from in vitro somatic embryogenesis (SE) induction in plants as a reference compared to virus-induced profiles can identify differential virus signatures that link to harmful reprogramming. A standard profile of selected genes named ‘ReprogVirus’ was proposed for in vitro-scanning of early virus-induced reprogramming in critical primary infected cells/tissues as target trait. For data collection, the ‘ReprogVirus platform’ was initiated. This initiative aims to identify in a common effort across scientific boundaries critical virus footprints from diverse virus origins and variants as a basis for anti-viral strategy design. This approach is open for validation and extension. In the present study, we initiated validation by experimental transcriptome data available in public domain combined with advancing plant wet lab research. We compared plant-adapted transcriptomes according to ‘RegroVirus’ complemented by alternative oxidase (AOX) genes during de novo programming under SE-inducing conditions with in vitro corona virus-induced transcriptome profiles. This approach enabled identifying a major complex trait for early de novo programming during SARS-CoV-2 infection, called ‘CoV-MAC-TED’. It consists of unbalanced ROS/RNS levels, which are connected to increased aerobic fermentation that links to alpha-tubulin-based cell restructuration and progression of cell cycle. We conclude that anti-viral/anti-SARS-CoV-2 strategies need to rigorously target ‘CoV-MAC-TED’ in primary infected nose and mouth cells through prophylactic and very early therapeutic strategies. We also discuss potential strategies in the view of the beneficial role of AOX for resilient behavior in plants. Furthermore, following the general observation that ROS/RNS equilibration/redox homeostasis is of utmost importance at the very beginning of viral infection, we highlight that ‘de-stressing’ disease and social handling should be seen as essential part of anti-viral/anti-SARS-CoV-2 strategies.JC is grateful to CNPq for the Researcher fellowship (CNPq grant 309795/2017-6). GM is grateful to UGC, India, for doctoral grant from BSR fellowship. GM, RS, and BA-S acknowledge support for academic cooperation and researcher’s mobility by the India-Portugal Bilateral Cooperation Program (2013-2015), funded by “Fundação para a Ciência e Tecnologia” (FCT), Portugal, and the Department of Science and Technology (DST), India. BR and SS acknowledge the infrastructure and stay support provided by DBT-TDNBC-DEAKIN – Research Network Across continents for learning and innovation (DTD-RNA) for AMF related work at The Energy and Resources Institute, TERI, India. CN acknowledges the international scientific network BIOALI-CYTED, which contributed to establish FunCROP contacts. KT is grateful to CNPq for the Doctoral fellowship. SA is grateful to CAPES for the Doctoral fellowship. KG, MO, and BA-S acknowledge support by the India-Portugal Bilateral Cooperation Program ‘DST/INT/Portugal/P-03/2017’. MO Research is partially supported by National Funds through. FCT. Fundação para a Ciência e a Tecnologia, projects UIDB/04674/2020 (CIMA). BA-S wants to thank Dr. Natascha Sommer for helpful discussions and comments on the manuscript during its development on the background of her experience as medical doctor in the group of Prof. Dr. Norbert Weissmann, Chair for ‘Molecular Mechanisms of Emphysema, Hypoxia and Lung Aging’ at the Universities of Giessen and Marburg Lung Center (UGMLC), Germany, and as investigator involved in mitochondrial redox biology also by help of transgenic AOX-mice. BAS acknowledges the strong engagement and clear view of Dr. Kim Berit Lewerenz-Kemper transmitted in support of our approach and final wider conclusions that is based not only on her overall life experience and view but also on her coronavirus-dominated experience as medical doctor in emergency service at the hospital ‘Universitätsklinikum Eppendorf’ in Hamburg, Germany. Furthermore, we want to appreciate encouragement and comments on the manuscript by Dr. Elisete Santos Macedo, Prof. Dr. Ashwani Kumar (emeritus at the University of Jaipur, India) and Dr. Nidhi Gupta, all are engaged plant scientists, educators, book writers and members of the FunCROP net and accompanied developing this approach from beginning. Finally, the authors thank Sebastian Schaffer, Nutritional Scientist with special knowledge in anti-oxidant food ingredients and their relevance in aging and as neuroprotective agents, who currently founded ACADELION Scientific and Medical Communications, for his carefully reading and helpful editing of the manuscript.Frontiers Media2022-12-07T12:08:34Z2022-12-072021-07-17T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/32855http://hdl.handle.net/10174/32855https://doi.org/10.3389/fimmu.2021.673692engCosta JH, Mohanapriya G, Bharadwaj R, Noceda C, Thiers KLL, Aziz S, Srivastava S, Oliveira M, Gupta KJ, Kumari A, Sircar D, Kumar SR, Achra A, Sathishkumar R, Adholeya A and Arnholdt-Schmitt B (2021) ROS/RNS Balancing, Aerobic Fermentation Regulation and Cell Cycle Control – a Complex Early Trait (‘CoV-MAC-TED’) for Combating SARS-CoV-2-Induced Cell Reprogramming. Front. Immunol. 12:673692. doi: 10.3389/fimmu.2021.673692https://www.frontiersin.org/articles/10.3389/fimmu.2021.673692/fullhelio.costa@ufc.brndndndndndndmmo@uevora.ptndndndndndndndbiarnaflora@gmail.com234Costa, José HélioMohanapriya, GunasekaranBharadwaj, RevuruNoceda, CarlosThiers, Karine Leitão LimaAziz, ShahidSrivastava, ShivaniOliveira, ManuelaGupta, Kapuganti JagadisKumari, AprajitaSircar, DebabrataKumar, Sarma RajeevAchra, ArvindSathishkumar, RamalingamAdholeya, AlokArnholdt-Schmitt, Birgitinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:33:36Zoai:dspace.uevora.pt:10174/32855Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:21:38.716202Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
ROS/RNS Balancing, Aerobic Fermentation Regulation and Cell Cycle Control – a Complex Early Trait (‘CoV-MAC-TED’) for Combating SARS-CoV-2-Induced Cell Reprogramming |
title |
ROS/RNS Balancing, Aerobic Fermentation Regulation and Cell Cycle Control – a Complex Early Trait (‘CoV-MAC-TED’) for Combating SARS-CoV-2-Induced Cell Reprogramming |
spellingShingle |
ROS/RNS Balancing, Aerobic Fermentation Regulation and Cell Cycle Control – a Complex Early Trait (‘CoV-MAC-TED’) for Combating SARS-CoV-2-Induced Cell Reprogramming Costa, José Hélio SARS-CoV-2 redox biology alternative oxidase tubulin mTOR melatonin repurposing drugs |
title_short |
ROS/RNS Balancing, Aerobic Fermentation Regulation and Cell Cycle Control – a Complex Early Trait (‘CoV-MAC-TED’) for Combating SARS-CoV-2-Induced Cell Reprogramming |
title_full |
ROS/RNS Balancing, Aerobic Fermentation Regulation and Cell Cycle Control – a Complex Early Trait (‘CoV-MAC-TED’) for Combating SARS-CoV-2-Induced Cell Reprogramming |
title_fullStr |
ROS/RNS Balancing, Aerobic Fermentation Regulation and Cell Cycle Control – a Complex Early Trait (‘CoV-MAC-TED’) for Combating SARS-CoV-2-Induced Cell Reprogramming |
title_full_unstemmed |
ROS/RNS Balancing, Aerobic Fermentation Regulation and Cell Cycle Control – a Complex Early Trait (‘CoV-MAC-TED’) for Combating SARS-CoV-2-Induced Cell Reprogramming |
title_sort |
ROS/RNS Balancing, Aerobic Fermentation Regulation and Cell Cycle Control – a Complex Early Trait (‘CoV-MAC-TED’) for Combating SARS-CoV-2-Induced Cell Reprogramming |
author |
Costa, José Hélio |
author_facet |
Costa, José Hélio Mohanapriya, Gunasekaran Bharadwaj, Revuru Noceda, Carlos Thiers, Karine Leitão Lima Aziz, Shahid Srivastava, Shivani Oliveira, Manuela Gupta, Kapuganti Jagadis Kumari, Aprajita Sircar, Debabrata Kumar, Sarma Rajeev Achra, Arvind Sathishkumar, Ramalingam Adholeya, Alok Arnholdt-Schmitt, Birgit |
author_role |
author |
author2 |
Mohanapriya, Gunasekaran Bharadwaj, Revuru Noceda, Carlos Thiers, Karine Leitão Lima Aziz, Shahid Srivastava, Shivani Oliveira, Manuela Gupta, Kapuganti Jagadis Kumari, Aprajita Sircar, Debabrata Kumar, Sarma Rajeev Achra, Arvind Sathishkumar, Ramalingam Adholeya, Alok Arnholdt-Schmitt, Birgit |
author2_role |
author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Costa, José Hélio Mohanapriya, Gunasekaran Bharadwaj, Revuru Noceda, Carlos Thiers, Karine Leitão Lima Aziz, Shahid Srivastava, Shivani Oliveira, Manuela Gupta, Kapuganti Jagadis Kumari, Aprajita Sircar, Debabrata Kumar, Sarma Rajeev Achra, Arvind Sathishkumar, Ramalingam Adholeya, Alok Arnholdt-Schmitt, Birgit |
dc.subject.por.fl_str_mv |
SARS-CoV-2 redox biology alternative oxidase tubulin mTOR melatonin repurposing drugs |
topic |
SARS-CoV-2 redox biology alternative oxidase tubulin mTOR melatonin repurposing drugs |
description |
In a perspective entitled ‘From plant survival under severe stress to anti-viral human defense’ we raised and justified the hypothesis that transcript level profiles of justified target genes established from in vitro somatic embryogenesis (SE) induction in plants as a reference compared to virus-induced profiles can identify differential virus signatures that link to harmful reprogramming. A standard profile of selected genes named ‘ReprogVirus’ was proposed for in vitro-scanning of early virus-induced reprogramming in critical primary infected cells/tissues as target trait. For data collection, the ‘ReprogVirus platform’ was initiated. This initiative aims to identify in a common effort across scientific boundaries critical virus footprints from diverse virus origins and variants as a basis for anti-viral strategy design. This approach is open for validation and extension. In the present study, we initiated validation by experimental transcriptome data available in public domain combined with advancing plant wet lab research. We compared plant-adapted transcriptomes according to ‘RegroVirus’ complemented by alternative oxidase (AOX) genes during de novo programming under SE-inducing conditions with in vitro corona virus-induced transcriptome profiles. This approach enabled identifying a major complex trait for early de novo programming during SARS-CoV-2 infection, called ‘CoV-MAC-TED’. It consists of unbalanced ROS/RNS levels, which are connected to increased aerobic fermentation that links to alpha-tubulin-based cell restructuration and progression of cell cycle. We conclude that anti-viral/anti-SARS-CoV-2 strategies need to rigorously target ‘CoV-MAC-TED’ in primary infected nose and mouth cells through prophylactic and very early therapeutic strategies. We also discuss potential strategies in the view of the beneficial role of AOX for resilient behavior in plants. Furthermore, following the general observation that ROS/RNS equilibration/redox homeostasis is of utmost importance at the very beginning of viral infection, we highlight that ‘de-stressing’ disease and social handling should be seen as essential part of anti-viral/anti-SARS-CoV-2 strategies. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07-17T00:00:00Z 2022-12-07T12:08:34Z 2022-12-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/32855 http://hdl.handle.net/10174/32855 https://doi.org/10.3389/fimmu.2021.673692 |
url |
http://hdl.handle.net/10174/32855 https://doi.org/10.3389/fimmu.2021.673692 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Costa JH, Mohanapriya G, Bharadwaj R, Noceda C, Thiers KLL, Aziz S, Srivastava S, Oliveira M, Gupta KJ, Kumari A, Sircar D, Kumar SR, Achra A, Sathishkumar R, Adholeya A and Arnholdt-Schmitt B (2021) ROS/RNS Balancing, Aerobic Fermentation Regulation and Cell Cycle Control – a Complex Early Trait (‘CoV-MAC-TED’) for Combating SARS-CoV-2-Induced Cell Reprogramming. Front. Immunol. 12:673692. doi: 10.3389/fimmu.2021.673692 https://www.frontiersin.org/articles/10.3389/fimmu.2021.673692/full helio.costa@ufc.br nd nd nd nd nd nd mmo@uevora.pt nd nd nd nd nd nd nd biarnaflora@gmail.com 234 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Frontiers Media |
publisher.none.fl_str_mv |
Frontiers Media |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799136698115817472 |