Improvement of the slide culture technique for the assessment of yeast viability
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/46728 |
Resumo: | This work aimed to improve the slide culture technique (SCT) for the assessment of yeast viability. Thus, all the steps of the SCT were standardized: a sample of 20 L containing 1 × 105 cells/mL was placed in a ~ 20 × 20 mm YPD agar block and incubated for 1624 h, at 25°C. It was proposed the use of calcofluor white (CFW) to facilitate the microscopic observation of yeast cells. The viability of cell populations in different physiological states (healthy, ethanol stressed and starved cells), assessed by SCT (without or with CFW), did not differ significantly (p < 0.01). In addition, the viability of healthy and ethanol stressed cells determined by the SCT and the standard plate count technique (PCT) did not differ significantly (p < 0.01). In conclusion, the improved SCT is a fast and reliable alternative to PCT for the evaluation of yeast viability in research and in the industry. |
id |
RCAP_e141c7e799139a0026636d8a5f3da8aa |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/46728 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Improvement of the slide culture technique for the assessment of yeast viabilityEthanol stressMicro-colony countSaccharomyces cerevisiaeStarvationViabilityScience & TechnologyThis work aimed to improve the slide culture technique (SCT) for the assessment of yeast viability. Thus, all the steps of the SCT were standardized: a sample of 20 L containing 1 × 105 cells/mL was placed in a ~ 20 × 20 mm YPD agar block and incubated for 1624 h, at 25°C. It was proposed the use of calcofluor white (CFW) to facilitate the microscopic observation of yeast cells. The viability of cell populations in different physiological states (healthy, ethanol stressed and starved cells), assessed by SCT (without or with CFW), did not differ significantly (p < 0.01). In addition, the viability of healthy and ethanol stressed cells determined by the SCT and the standard plate count technique (PCT) did not differ significantly (p < 0.01). In conclusion, the improved SCT is a fast and reliable alternative to PCT for the evaluation of yeast viability in research and in the industry.This study was supported by the Portuguese Foundation for Science and Technology under the scope of the strategic founding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and the Project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462). Manuela D. Machado and Catia A. Sousa gratefully acknowledge the post-doctoral (SFRH/BPD/72816/2010) and the doctoral grants (SFRH/BD/101452/2014), from FCT, respectively.info:eu-repo/semantics/publishedVersionInstitute of Brewing & DistillingUniversidade do MinhoCruyt, FrederikSousa, C. A.Machado, Manuela D.Soares, Eduardo V.2017-03-162017-03-16T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/46728engCruyt, Frederik; Sousa, C. A.; Machado, Manuela D.; Soares, Eduardo V., Improvement of the slide culture technique for the assessment of yeast viability. Journal of the Institute of Brewing, 123(1), 39-44, 20170046-975010.1002/jib.400http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)2050-0416info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:13:41Zoai:repositorium.sdum.uminho.pt:1822/46728Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:05:51.380588Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Improvement of the slide culture technique for the assessment of yeast viability |
title |
Improvement of the slide culture technique for the assessment of yeast viability |
spellingShingle |
Improvement of the slide culture technique for the assessment of yeast viability Cruyt, Frederik Ethanol stress Micro-colony count Saccharomyces cerevisiae Starvation Viability Science & Technology |
title_short |
Improvement of the slide culture technique for the assessment of yeast viability |
title_full |
Improvement of the slide culture technique for the assessment of yeast viability |
title_fullStr |
Improvement of the slide culture technique for the assessment of yeast viability |
title_full_unstemmed |
Improvement of the slide culture technique for the assessment of yeast viability |
title_sort |
Improvement of the slide culture technique for the assessment of yeast viability |
author |
Cruyt, Frederik |
author_facet |
Cruyt, Frederik Sousa, C. A. Machado, Manuela D. Soares, Eduardo V. |
author_role |
author |
author2 |
Sousa, C. A. Machado, Manuela D. Soares, Eduardo V. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Cruyt, Frederik Sousa, C. A. Machado, Manuela D. Soares, Eduardo V. |
dc.subject.por.fl_str_mv |
Ethanol stress Micro-colony count Saccharomyces cerevisiae Starvation Viability Science & Technology |
topic |
Ethanol stress Micro-colony count Saccharomyces cerevisiae Starvation Viability Science & Technology |
description |
This work aimed to improve the slide culture technique (SCT) for the assessment of yeast viability. Thus, all the steps of the SCT were standardized: a sample of 20 L containing 1 × 105 cells/mL was placed in a ~ 20 × 20 mm YPD agar block and incubated for 1624 h, at 25°C. It was proposed the use of calcofluor white (CFW) to facilitate the microscopic observation of yeast cells. The viability of cell populations in different physiological states (healthy, ethanol stressed and starved cells), assessed by SCT (without or with CFW), did not differ significantly (p < 0.01). In addition, the viability of healthy and ethanol stressed cells determined by the SCT and the standard plate count technique (PCT) did not differ significantly (p < 0.01). In conclusion, the improved SCT is a fast and reliable alternative to PCT for the evaluation of yeast viability in research and in the industry. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03-16 2017-03-16T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/46728 |
url |
http://hdl.handle.net/1822/46728 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cruyt, Frederik; Sousa, C. A.; Machado, Manuela D.; Soares, Eduardo V., Improvement of the slide culture technique for the assessment of yeast viability. Journal of the Institute of Brewing, 123(1), 39-44, 2017 0046-9750 10.1002/jib.400 http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)2050-0416 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Institute of Brewing & Distilling |
publisher.none.fl_str_mv |
Institute of Brewing & Distilling |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132471893164032 |