The impact of CdSe/ZnS Quantum Dots in cells of Medicago sativa in suspension culture

Detalhes bibliográficos
Autor(a) principal: Oliva, Abel
Data de Publicação: 2010
Outros Autores: Fevereiro, Pedro, Vaz Patto, Carlota, Maycock, Christopher, Malho, Rui, Tomaz, Leonor, Miguel, Ana, Santos, Ana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/4639
Resumo: Abstract Background: Nanotechnology has the potential to provide agriculture with new tools that may be used in the rapid detection and molecular treatment of diseases and enhancement of plant ability to absorb nutrients, among others. Data on nanoparticle toxicity in plants is largely heterogeneous with a diversity of physicochemical parameters reported, which difficult generalizations. Here a cell biology approach was used to evaluate the impact of Quantum Dots (QDs) nanocrystals on plant cells, including their effect on cell growth, cell viability, oxidative stress and ROS accumulation, besides their cytomobility. Results: A plant cell suspension culture of Medicago sativa was settled for the assessment of the impact of the addition of mercaptopropanoic acid coated CdSe/ZnS QDs. Cell growth was significantly reduced when 100 mM of mercaptopropanoic acid -QDs was added during the exponential growth phase, with less than 50% of the cells viable 72 hours after mercaptopropanoic acid -QDs addition. They were up taken by Medicago sativa cells and accumulated in the cytoplasm and nucleus as revealed by optical thin confocal imaging. As part of the cellular response to internalization, Medicago sativa cells were found to increase the production of Reactive Oxygen Species (ROS) in a dose and time dependent manner. Using the fluorescent dye H2DCFDA it was observable that mercaptopropanoic acid-QDs concentrations between 5-180 nM led to a progressive and linear increase of ROS accumulation. Conclusions: Our results showed that the extent of mercaptopropanoic acid coated CdSe/ZnS QDs cytotoxicity in plant cells is dependent upon a number of factors including QDs properties, dose and the environmental conditions of administration and that, for Medicago sativa cells, a safe range of 1-5 nM should not be exceeded for biological applications.
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spelling The impact of CdSe/ZnS Quantum Dots in cells of Medicago sativa in suspension cultureQuantum DotsMedicago sativaNanotoxicologyROSAbstract Background: Nanotechnology has the potential to provide agriculture with new tools that may be used in the rapid detection and molecular treatment of diseases and enhancement of plant ability to absorb nutrients, among others. Data on nanoparticle toxicity in plants is largely heterogeneous with a diversity of physicochemical parameters reported, which difficult generalizations. Here a cell biology approach was used to evaluate the impact of Quantum Dots (QDs) nanocrystals on plant cells, including their effect on cell growth, cell viability, oxidative stress and ROS accumulation, besides their cytomobility. Results: A plant cell suspension culture of Medicago sativa was settled for the assessment of the impact of the addition of mercaptopropanoic acid coated CdSe/ZnS QDs. Cell growth was significantly reduced when 100 mM of mercaptopropanoic acid -QDs was added during the exponential growth phase, with less than 50% of the cells viable 72 hours after mercaptopropanoic acid -QDs addition. They were up taken by Medicago sativa cells and accumulated in the cytoplasm and nucleus as revealed by optical thin confocal imaging. As part of the cellular response to internalization, Medicago sativa cells were found to increase the production of Reactive Oxygen Species (ROS) in a dose and time dependent manner. Using the fluorescent dye H2DCFDA it was observable that mercaptopropanoic acid-QDs concentrations between 5-180 nM led to a progressive and linear increase of ROS accumulation. Conclusions: Our results showed that the extent of mercaptopropanoic acid coated CdSe/ZnS QDs cytotoxicity in plant cells is dependent upon a number of factors including QDs properties, dose and the environmental conditions of administration and that, for Medicago sativa cells, a safe range of 1-5 nM should not be exceeded for biological applications.This work was supported by the project “Development of ultra-sensitive detection methods and plant nano-vaccines for the fungi Fusarium spp. using nanotechnological devices” Iberian Capacitation Program in Nanotechnologies: Call 2006/2007”.BioMed CentralRUNOliva, AbelFevereiro, PedroVaz Patto, CarlotaMaycock, ChristopherMalho, RuiTomaz, LeonorMiguel, AnaSantos, Ana2010-12-20T11:23:01Z2010-082010-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/4639engSantos et al.: The impact of CdSe/ZnS Quantum Dots in cells of Medicago sativa in suspension culture. Journal of Nanobiotechnology 2010 8:24.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:34:32Zoai:run.unl.pt:10362/4639Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:15:47.746717Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The impact of CdSe/ZnS Quantum Dots in cells of Medicago sativa in suspension culture
title The impact of CdSe/ZnS Quantum Dots in cells of Medicago sativa in suspension culture
spellingShingle The impact of CdSe/ZnS Quantum Dots in cells of Medicago sativa in suspension culture
Oliva, Abel
Quantum Dots
Medicago sativa
Nanotoxicology
ROS
title_short The impact of CdSe/ZnS Quantum Dots in cells of Medicago sativa in suspension culture
title_full The impact of CdSe/ZnS Quantum Dots in cells of Medicago sativa in suspension culture
title_fullStr The impact of CdSe/ZnS Quantum Dots in cells of Medicago sativa in suspension culture
title_full_unstemmed The impact of CdSe/ZnS Quantum Dots in cells of Medicago sativa in suspension culture
title_sort The impact of CdSe/ZnS Quantum Dots in cells of Medicago sativa in suspension culture
author Oliva, Abel
author_facet Oliva, Abel
Fevereiro, Pedro
Vaz Patto, Carlota
Maycock, Christopher
Malho, Rui
Tomaz, Leonor
Miguel, Ana
Santos, Ana
author_role author
author2 Fevereiro, Pedro
Vaz Patto, Carlota
Maycock, Christopher
Malho, Rui
Tomaz, Leonor
Miguel, Ana
Santos, Ana
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Oliva, Abel
Fevereiro, Pedro
Vaz Patto, Carlota
Maycock, Christopher
Malho, Rui
Tomaz, Leonor
Miguel, Ana
Santos, Ana
dc.subject.por.fl_str_mv Quantum Dots
Medicago sativa
Nanotoxicology
ROS
topic Quantum Dots
Medicago sativa
Nanotoxicology
ROS
description Abstract Background: Nanotechnology has the potential to provide agriculture with new tools that may be used in the rapid detection and molecular treatment of diseases and enhancement of plant ability to absorb nutrients, among others. Data on nanoparticle toxicity in plants is largely heterogeneous with a diversity of physicochemical parameters reported, which difficult generalizations. Here a cell biology approach was used to evaluate the impact of Quantum Dots (QDs) nanocrystals on plant cells, including their effect on cell growth, cell viability, oxidative stress and ROS accumulation, besides their cytomobility. Results: A plant cell suspension culture of Medicago sativa was settled for the assessment of the impact of the addition of mercaptopropanoic acid coated CdSe/ZnS QDs. Cell growth was significantly reduced when 100 mM of mercaptopropanoic acid -QDs was added during the exponential growth phase, with less than 50% of the cells viable 72 hours after mercaptopropanoic acid -QDs addition. They were up taken by Medicago sativa cells and accumulated in the cytoplasm and nucleus as revealed by optical thin confocal imaging. As part of the cellular response to internalization, Medicago sativa cells were found to increase the production of Reactive Oxygen Species (ROS) in a dose and time dependent manner. Using the fluorescent dye H2DCFDA it was observable that mercaptopropanoic acid-QDs concentrations between 5-180 nM led to a progressive and linear increase of ROS accumulation. Conclusions: Our results showed that the extent of mercaptopropanoic acid coated CdSe/ZnS QDs cytotoxicity in plant cells is dependent upon a number of factors including QDs properties, dose and the environmental conditions of administration and that, for Medicago sativa cells, a safe range of 1-5 nM should not be exceeded for biological applications.
publishDate 2010
dc.date.none.fl_str_mv 2010-12-20T11:23:01Z
2010-08
2010-08-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/4639
url http://hdl.handle.net/10362/4639
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Santos et al.: The impact of CdSe/ZnS Quantum Dots in cells of Medicago sativa in suspension culture. Journal of Nanobiotechnology 2010 8:24.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
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