Different ability of multidrug-resistant and-sensitive counterpart cells to release and capture extracellular vesicles

Detalhes bibliográficos
Autor(a) principal: Sousa, D
Data de Publicação: 2021
Outros Autores: Lima, RT, Lopes-Rodrigues, V, Gonzalez, E, Royo, F, Xavier, CPR, Falcón-Pérez, JM, Vasconcelos, MH
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/10216/154873
Resumo: Cancer multidrug resistance (MDR) is one of the main challenges for cancer treatment efficacy. MDR is a phenomenon by which tumor cells become resistant to several unrelated drugs. Some studies have previously described the important role of extracellular vesicles (EVs) in the dissemination of a MDR phenotype. EVs’ cargo may include different players of MDR, such as microRNAS and drug-efflux pumps, which may be transferred from donor MDR cells to recipient drug-sensitive counterparts. The present work aimed to: (i) compare the ability of drug-sensitive and their MDR counterpart cells to release and capture EVs and (ii) study and relate those differences with possible distinct fate of the endocytic pathway in these counterpart cells. Our results showed that MDR cells released more EVs than their drug-sensitive counterparts and also that the drug-sensitive cells captured more EVs than their MDR counterparts. This difference in the release and capture of EVs may be associated with differences in the endocytic pathway between drug-sensitive and MDR cells. Importantly, manipulation of the recycling pathway influenced the response of drug-sensitive cells to doxorubicin treatment.
id RCAP_e1492b70687c12e77d7d39e15ea765f7
oai_identifier_str oai:repositorio-aberto.up.pt:10216/154873
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Different ability of multidrug-resistant and-sensitive counterpart cells to release and capture extracellular vesiclesCancer multidrug resistanceEndocytic pathwayExtracellular vesiclesCancer multidrug resistance (MDR) is one of the main challenges for cancer treatment efficacy. MDR is a phenomenon by which tumor cells become resistant to several unrelated drugs. Some studies have previously described the important role of extracellular vesicles (EVs) in the dissemination of a MDR phenotype. EVs’ cargo may include different players of MDR, such as microRNAS and drug-efflux pumps, which may be transferred from donor MDR cells to recipient drug-sensitive counterparts. The present work aimed to: (i) compare the ability of drug-sensitive and their MDR counterpart cells to release and capture EVs and (ii) study and relate those differences with possible distinct fate of the endocytic pathway in these counterpart cells. Our results showed that MDR cells released more EVs than their drug-sensitive counterparts and also that the drug-sensitive cells captured more EVs than their MDR counterparts. This difference in the release and capture of EVs may be associated with differences in the endocytic pathway between drug-sensitive and MDR cells. Importantly, manipulation of the recycling pathway influenced the response of drug-sensitive cells to doxorubicin treatment.MDPI20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/154873eng2073-440910.3390/cells10112886Sousa, DLima, RTLopes-Rodrigues, VGonzalez, ERoyo, FXavier, CPRFalcón-Pérez, JMVasconcelos, MHinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-09-27T09:37:06Zoai:repositorio-aberto.up.pt:10216/154873Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-09-27T09:37:06Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Different ability of multidrug-resistant and-sensitive counterpart cells to release and capture extracellular vesicles
title Different ability of multidrug-resistant and-sensitive counterpart cells to release and capture extracellular vesicles
spellingShingle Different ability of multidrug-resistant and-sensitive counterpart cells to release and capture extracellular vesicles
Sousa, D
Cancer multidrug resistance
Endocytic pathway
Extracellular vesicles
title_short Different ability of multidrug-resistant and-sensitive counterpart cells to release and capture extracellular vesicles
title_full Different ability of multidrug-resistant and-sensitive counterpart cells to release and capture extracellular vesicles
title_fullStr Different ability of multidrug-resistant and-sensitive counterpart cells to release and capture extracellular vesicles
title_full_unstemmed Different ability of multidrug-resistant and-sensitive counterpart cells to release and capture extracellular vesicles
title_sort Different ability of multidrug-resistant and-sensitive counterpart cells to release and capture extracellular vesicles
author Sousa, D
author_facet Sousa, D
Lima, RT
Lopes-Rodrigues, V
Gonzalez, E
Royo, F
Xavier, CPR
Falcón-Pérez, JM
Vasconcelos, MH
author_role author
author2 Lima, RT
Lopes-Rodrigues, V
Gonzalez, E
Royo, F
Xavier, CPR
Falcón-Pérez, JM
Vasconcelos, MH
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sousa, D
Lima, RT
Lopes-Rodrigues, V
Gonzalez, E
Royo, F
Xavier, CPR
Falcón-Pérez, JM
Vasconcelos, MH
dc.subject.por.fl_str_mv Cancer multidrug resistance
Endocytic pathway
Extracellular vesicles
topic Cancer multidrug resistance
Endocytic pathway
Extracellular vesicles
description Cancer multidrug resistance (MDR) is one of the main challenges for cancer treatment efficacy. MDR is a phenomenon by which tumor cells become resistant to several unrelated drugs. Some studies have previously described the important role of extracellular vesicles (EVs) in the dissemination of a MDR phenotype. EVs’ cargo may include different players of MDR, such as microRNAS and drug-efflux pumps, which may be transferred from donor MDR cells to recipient drug-sensitive counterparts. The present work aimed to: (i) compare the ability of drug-sensitive and their MDR counterpart cells to release and capture EVs and (ii) study and relate those differences with possible distinct fate of the endocytic pathway in these counterpart cells. Our results showed that MDR cells released more EVs than their drug-sensitive counterparts and also that the drug-sensitive cells captured more EVs than their MDR counterparts. This difference in the release and capture of EVs may be associated with differences in the endocytic pathway between drug-sensitive and MDR cells. Importantly, manipulation of the recycling pathway influenced the response of drug-sensitive cells to doxorubicin treatment.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/10216/154873
url https://hdl.handle.net/10216/154873
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2073-4409
10.3390/cells10112886
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
_version_ 1817548387553116160