Effect of purified trichoderma reesei cellulases on formation of cotton powder from cotton fabric

Detalhes bibliográficos
Autor(a) principal: Heikinheimo, Lea
Data de Publicação: 2003
Outros Autores: Miettinen-Oinonen, Arja, Paulo, Artur Cavaco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/1983
Resumo: The mode of action of monocomponent purified Trichoderma reesei cellobiohydrolases (CBHI and CBHII) and endoglucanases (EGI and EGII) on cotton fabrics was studied by analyzing the weight loss of the fabric, the reducing sugars, the soluble oligosaccharides and the molecular weight of the cotton powder formed. The impact of mechanical action on these factors was also evaluated. EGI and EGII released the highest amounts of reducing sugars and soluble oligosaccharides in both treatments with or without additional mechanical action. After cellulase treatment without additional mechanical action, all of the cellulases were found to have reduced the molecular weight of cotton poplin powder. When mechanical action was combined with enzyme treatments, only EGII reduced the molecular weight. The weight loss of EG-treated fabrics was clearly higher than the weight loss of CBH-treated fabrics with both low and high mechanical action levels.
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spelling Effect of purified trichoderma reesei cellulases on formation of cotton powder from cotton fabricenzymesfibersmolecular weight distributionhigh performance liquid chromatography (HPLC)Science & TechnologyThe mode of action of monocomponent purified Trichoderma reesei cellobiohydrolases (CBHI and CBHII) and endoglucanases (EGI and EGII) on cotton fabrics was studied by analyzing the weight loss of the fabric, the reducing sugars, the soluble oligosaccharides and the molecular weight of the cotton powder formed. The impact of mechanical action on these factors was also evaluated. EGI and EGII released the highest amounts of reducing sugars and soluble oligosaccharides in both treatments with or without additional mechanical action. After cellulase treatment without additional mechanical action, all of the cellulases were found to have reduced the molecular weight of cotton poplin powder. When mechanical action was combined with enzyme treatments, only EGII reduced the molecular weight. The weight loss of EG-treated fabrics was clearly higher than the weight loss of CBH-treated fabrics with both low and high mechanical action levels.WileyUniversidade do MinhoHeikinheimo, LeaMiettinen-Oinonen, ArjaPaulo, Artur Cavaco20032003-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/1983eng"Journal of applied polymer science". ISSN 0021-8995. 90 (2003) 1917-1922.0021-899510.1002/app.12868info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:00:34Zoai:repositorium.sdum.uminho.pt:1822/1983Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:50:26.208317Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Effect of purified trichoderma reesei cellulases on formation of cotton powder from cotton fabric
title Effect of purified trichoderma reesei cellulases on formation of cotton powder from cotton fabric
spellingShingle Effect of purified trichoderma reesei cellulases on formation of cotton powder from cotton fabric
Heikinheimo, Lea
enzymes
fibers
molecular weight distribution
high performance liquid chromatography (HPLC)
Science & Technology
title_short Effect of purified trichoderma reesei cellulases on formation of cotton powder from cotton fabric
title_full Effect of purified trichoderma reesei cellulases on formation of cotton powder from cotton fabric
title_fullStr Effect of purified trichoderma reesei cellulases on formation of cotton powder from cotton fabric
title_full_unstemmed Effect of purified trichoderma reesei cellulases on formation of cotton powder from cotton fabric
title_sort Effect of purified trichoderma reesei cellulases on formation of cotton powder from cotton fabric
author Heikinheimo, Lea
author_facet Heikinheimo, Lea
Miettinen-Oinonen, Arja
Paulo, Artur Cavaco
author_role author
author2 Miettinen-Oinonen, Arja
Paulo, Artur Cavaco
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Heikinheimo, Lea
Miettinen-Oinonen, Arja
Paulo, Artur Cavaco
dc.subject.por.fl_str_mv enzymes
fibers
molecular weight distribution
high performance liquid chromatography (HPLC)
Science & Technology
topic enzymes
fibers
molecular weight distribution
high performance liquid chromatography (HPLC)
Science & Technology
description The mode of action of monocomponent purified Trichoderma reesei cellobiohydrolases (CBHI and CBHII) and endoglucanases (EGI and EGII) on cotton fabrics was studied by analyzing the weight loss of the fabric, the reducing sugars, the soluble oligosaccharides and the molecular weight of the cotton powder formed. The impact of mechanical action on these factors was also evaluated. EGI and EGII released the highest amounts of reducing sugars and soluble oligosaccharides in both treatments with or without additional mechanical action. After cellulase treatment without additional mechanical action, all of the cellulases were found to have reduced the molecular weight of cotton poplin powder. When mechanical action was combined with enzyme treatments, only EGII reduced the molecular weight. The weight loss of EG-treated fabrics was clearly higher than the weight loss of CBH-treated fabrics with both low and high mechanical action levels.
publishDate 2003
dc.date.none.fl_str_mv 2003
2003-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/1983
url http://hdl.handle.net/1822/1983
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Journal of applied polymer science". ISSN 0021-8995. 90 (2003) 1917-1922.
0021-8995
10.1002/app.12868
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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