MMP-9/RECK imbalance: a mechanism associated with high-grade cervical lesions and genital infection by Human Papillomavirus (HPV) and Chlamydia trachomatis

Detalhes bibliográficos
Autor(a) principal: Discacciati, Michelle G.
Data de Publicação: 2015
Outros Autores: Gimenes, Fabrícia, Pennacchi, Paula C., Flores, Fernanda Faião, Zeferino, Luiz C., Derchain, Sophie M., Teixeira, Júlio C., Costa, Maria C., Zonta, Marco, Longatto Filho, Adhemar
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/40247
Resumo: "Manuscript"
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spelling MMP-9/RECK imbalance: a mechanism associated with high-grade cervical lesions and genital infection by Human Papillomavirus (HPV) and Chlamydia trachomatisMatrix MetalloproteinasesRECK proteinCervical Intraepithelial NeoplasiaHuman papillomavirus 16Chlamydia trachomatisScience & Technology"Manuscript"BACKGROUND: Matrix metalloproteinases (MMP) are important enzymes in the tumor microenvironment associated with progression of cervical intraepithelial neoplasia (CIN) toward squamous cell carcinoma (SCC) of the cervix. However, the role of MMPs in the inflammatory process associated with Chlamydia trachomatis infection concomitant with the carcinogenic process driven by HPV has not yet been addressed. In the present study, we analyzed the state of the MMP-9-RECK axis in cervical carcinogenesis. METHODS: The levels of MMP-9 and RECK expression were analyzed by immunocytochemistry in liquid-based cytology samples from 136 women with high-grade cervical lesions (CIN2/CIN3) and cervical SCC diagnosed by LLETZ, and in 196 women without cervical neoplasia or CIN1. Real-time qPCR was performed to analyze expression of MMP-9 and RECK in 15 cervical samples. The presence of HPV-DNA and other genital pathogens was evaluated by PCR. RESULTS: We found a higher expression of MMP-9 [OR, 4.2; 95% confidence interval (CI), 2.2-7.8] and lower expression of RECK (OR, 0.4; 95% CI, 0.2-0.7) in women with CIN2/CIN3/SCC when compared with women from the control group (no neoplasia/CIN1). A statistically significant association was also found between MMP-9/RECK imbalance and infection by alpha-9 HPV and C. trachomatis. The prevalence of C. trachomatis infection was significantly higher in women with high-grade cervical disease (OR, 3.7; 95% CI, 1.3-11.3). CONCLUSIONS: MMP-9/RECK imbalance in cervical smears is significantly associated with high-grade cervical diseases and infection by alpha-9 HPV and C. trachomatis. IMPACT: MMP-9/RECK imbalance during cervical inflammation induced by C. trachomatis might play a role in HPV-mediated cervical carcinogenesis.This work was supported by Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP), numbers 2008/03232-1 (to L.L. Villa) and 2012/09746-2 (to M.G. Discacciati and S.S. Maria-Engler) and National Counsel of Technological and Scientific Development pharmaceutical innovation (CNPQ-INCT-if; to S.S. Maria-Engler).American Association for Cancer Research[et al.]Universidade do MinhoDiscacciati, Michelle G.Gimenes, FabríciaPennacchi, Paula C.Flores, Fernanda FaiãoZeferino, Luiz C.Derchain, Sophie M.Teixeira, Júlio C.Costa, Maria C.Zonta, MarcoLongatto Filho, Adhemar20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/40247engDiscacciati, M. G., Gimenes, F., Pennacchi, P. C., Faião-Flores, F., Zeferino, L. C., Derchain, S. M., . . . Maria-Engler, S. S. (2015). MMP-9/RECK imbalance: A mechanism associated with high-grade cervical lesions and genital infection by human papillomavirus and chlamydia trachomatis. Cancer Epidemiology Biomarkers and Prevention, 24(10), 1539-1547. doi: 10.1158/1055-9965.epi-15-04201055-996510.1158/1055-9965.EPI-15-042026261088http://cebp.aacrjournals.org/content/early/2015/09/06/1055-9965.EPI-15-0420.abstractinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:45:26Zoai:repositorium.sdum.uminho.pt:1822/40247Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:43:17.386098Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv MMP-9/RECK imbalance: a mechanism associated with high-grade cervical lesions and genital infection by Human Papillomavirus (HPV) and Chlamydia trachomatis
title MMP-9/RECK imbalance: a mechanism associated with high-grade cervical lesions and genital infection by Human Papillomavirus (HPV) and Chlamydia trachomatis
spellingShingle MMP-9/RECK imbalance: a mechanism associated with high-grade cervical lesions and genital infection by Human Papillomavirus (HPV) and Chlamydia trachomatis
Discacciati, Michelle G.
Matrix Metalloproteinases
RECK protein
Cervical Intraepithelial Neoplasia
Human papillomavirus 16
Chlamydia trachomatis
Science & Technology
title_short MMP-9/RECK imbalance: a mechanism associated with high-grade cervical lesions and genital infection by Human Papillomavirus (HPV) and Chlamydia trachomatis
title_full MMP-9/RECK imbalance: a mechanism associated with high-grade cervical lesions and genital infection by Human Papillomavirus (HPV) and Chlamydia trachomatis
title_fullStr MMP-9/RECK imbalance: a mechanism associated with high-grade cervical lesions and genital infection by Human Papillomavirus (HPV) and Chlamydia trachomatis
title_full_unstemmed MMP-9/RECK imbalance: a mechanism associated with high-grade cervical lesions and genital infection by Human Papillomavirus (HPV) and Chlamydia trachomatis
title_sort MMP-9/RECK imbalance: a mechanism associated with high-grade cervical lesions and genital infection by Human Papillomavirus (HPV) and Chlamydia trachomatis
author Discacciati, Michelle G.
author_facet Discacciati, Michelle G.
Gimenes, Fabrícia
Pennacchi, Paula C.
Flores, Fernanda Faião
Zeferino, Luiz C.
Derchain, Sophie M.
Teixeira, Júlio C.
Costa, Maria C.
Zonta, Marco
Longatto Filho, Adhemar
author_role author
author2 Gimenes, Fabrícia
Pennacchi, Paula C.
Flores, Fernanda Faião
Zeferino, Luiz C.
Derchain, Sophie M.
Teixeira, Júlio C.
Costa, Maria C.
Zonta, Marco
Longatto Filho, Adhemar
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv [et al.]
Universidade do Minho
dc.contributor.author.fl_str_mv Discacciati, Michelle G.
Gimenes, Fabrícia
Pennacchi, Paula C.
Flores, Fernanda Faião
Zeferino, Luiz C.
Derchain, Sophie M.
Teixeira, Júlio C.
Costa, Maria C.
Zonta, Marco
Longatto Filho, Adhemar
dc.subject.por.fl_str_mv Matrix Metalloproteinases
RECK protein
Cervical Intraepithelial Neoplasia
Human papillomavirus 16
Chlamydia trachomatis
Science & Technology
topic Matrix Metalloproteinases
RECK protein
Cervical Intraepithelial Neoplasia
Human papillomavirus 16
Chlamydia trachomatis
Science & Technology
description "Manuscript"
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/40247
url http://hdl.handle.net/1822/40247
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Discacciati, M. G., Gimenes, F., Pennacchi, P. C., Faião-Flores, F., Zeferino, L. C., Derchain, S. M., . . . Maria-Engler, S. S. (2015). MMP-9/RECK imbalance: A mechanism associated with high-grade cervical lesions and genital infection by human papillomavirus and chlamydia trachomatis. Cancer Epidemiology Biomarkers and Prevention, 24(10), 1539-1547. doi: 10.1158/1055-9965.epi-15-0420
1055-9965
10.1158/1055-9965.EPI-15-0420
26261088
http://cebp.aacrjournals.org/content/early/2015/09/06/1055-9965.EPI-15-0420.abstract
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Association for Cancer Research
publisher.none.fl_str_mv American Association for Cancer Research
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132988814917632

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