Trkb receptors modulation of glutamate release is limited to a subset of nerve terminals in the adult rat hippocampus

Detalhes bibliográficos
Autor(a) principal: Pereira, Daniela B.
Data de Publicação: 2006
Outros Autores: Rebola, Nelson, Rodrigues, Ricardo J., Cunha, Rodrigo A., Carvalho, Arsélio P., Duarte, Carlos B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/8320
https://doi.org/10.1002/jnr.20784
Resumo: Brain-derived neurotrophic factor (BDNF) modulates glutamatergic excitatory transmission in hippocampal primary cultures by acting at a presynaptic locus. Although it has been suggested that BDNF also modulates adult hippocampus glutamatergic transmission, this remains a matter of controversy. To clarify a putative role for this neurotrophin in the modulation of glutamate release we applied exogenous BDNF to isolated adult rat hippocampal nerve terminals. BDNF, at 100 ng/ml, potentiated by 25% the K+-evoked release of [3H]glutamate from hippocampal synaptosomes. The small effect of BDNF on [3H]glutamate release correlated with a modest increase in phospholipase Cgamma (PLCgamma) phosphorylation, and with the lack of effect of BDNF on extracellular-signal regulated kinase (ERK) and Akt phosphorylation. Immunocytochemistry studies demonstrated that only about one-third of glutamatergic synaptosomes were positive for TrkB immunoreactivity. Furthermore, biotinylation and subsynaptic fractionation studies showed that only one-fourth of total full-length TrkB was present at the plasma membrane, evenly distributed between the presynaptic active zone and the postsynaptic density. These results indicate that BDNF modulates synaptic transmission presynaptically in a small subset of hippocampal glutamatergic synapses that contain TrkB and that express the receptor on the plasma membrane. © 2006 Wiley-Liss, Inc.
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spelling Trkb receptors modulation of glutamate release is limited to a subset of nerve terminals in the adult rat hippocampusBrain-derived neurotrophic factor (BDNF) modulates glutamatergic excitatory transmission in hippocampal primary cultures by acting at a presynaptic locus. Although it has been suggested that BDNF also modulates adult hippocampus glutamatergic transmission, this remains a matter of controversy. To clarify a putative role for this neurotrophin in the modulation of glutamate release we applied exogenous BDNF to isolated adult rat hippocampal nerve terminals. BDNF, at 100 ng/ml, potentiated by 25% the K+-evoked release of [3H]glutamate from hippocampal synaptosomes. The small effect of BDNF on [3H]glutamate release correlated with a modest increase in phospholipase Cgamma (PLCgamma) phosphorylation, and with the lack of effect of BDNF on extracellular-signal regulated kinase (ERK) and Akt phosphorylation. Immunocytochemistry studies demonstrated that only about one-third of glutamatergic synaptosomes were positive for TrkB immunoreactivity. Furthermore, biotinylation and subsynaptic fractionation studies showed that only one-fourth of total full-length TrkB was present at the plasma membrane, evenly distributed between the presynaptic active zone and the postsynaptic density. These results indicate that BDNF modulates synaptic transmission presynaptically in a small subset of hippocampal glutamatergic synapses that contain TrkB and that express the receptor on the plasma membrane. © 2006 Wiley-Liss, Inc.2006info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/8320http://hdl.handle.net/10316/8320https://doi.org/10.1002/jnr.20784engJournal of Neuroscience Research. 83:5 (2006) 832-844Pereira, Daniela B.Rebola, NelsonRodrigues, Ricardo J.Cunha, Rodrigo A.Carvalho, Arsélio P.Duarte, Carlos B.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-05-29T09:41:52Zoai:estudogeral.uc.pt:10316/8320Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:55:31.796647Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Trkb receptors modulation of glutamate release is limited to a subset of nerve terminals in the adult rat hippocampus
title Trkb receptors modulation of glutamate release is limited to a subset of nerve terminals in the adult rat hippocampus
spellingShingle Trkb receptors modulation of glutamate release is limited to a subset of nerve terminals in the adult rat hippocampus
Pereira, Daniela B.
title_short Trkb receptors modulation of glutamate release is limited to a subset of nerve terminals in the adult rat hippocampus
title_full Trkb receptors modulation of glutamate release is limited to a subset of nerve terminals in the adult rat hippocampus
title_fullStr Trkb receptors modulation of glutamate release is limited to a subset of nerve terminals in the adult rat hippocampus
title_full_unstemmed Trkb receptors modulation of glutamate release is limited to a subset of nerve terminals in the adult rat hippocampus
title_sort Trkb receptors modulation of glutamate release is limited to a subset of nerve terminals in the adult rat hippocampus
author Pereira, Daniela B.
author_facet Pereira, Daniela B.
Rebola, Nelson
Rodrigues, Ricardo J.
Cunha, Rodrigo A.
Carvalho, Arsélio P.
Duarte, Carlos B.
author_role author
author2 Rebola, Nelson
Rodrigues, Ricardo J.
Cunha, Rodrigo A.
Carvalho, Arsélio P.
Duarte, Carlos B.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Pereira, Daniela B.
Rebola, Nelson
Rodrigues, Ricardo J.
Cunha, Rodrigo A.
Carvalho, Arsélio P.
Duarte, Carlos B.
description Brain-derived neurotrophic factor (BDNF) modulates glutamatergic excitatory transmission in hippocampal primary cultures by acting at a presynaptic locus. Although it has been suggested that BDNF also modulates adult hippocampus glutamatergic transmission, this remains a matter of controversy. To clarify a putative role for this neurotrophin in the modulation of glutamate release we applied exogenous BDNF to isolated adult rat hippocampal nerve terminals. BDNF, at 100 ng/ml, potentiated by 25% the K+-evoked release of [3H]glutamate from hippocampal synaptosomes. The small effect of BDNF on [3H]glutamate release correlated with a modest increase in phospholipase Cgamma (PLCgamma) phosphorylation, and with the lack of effect of BDNF on extracellular-signal regulated kinase (ERK) and Akt phosphorylation. Immunocytochemistry studies demonstrated that only about one-third of glutamatergic synaptosomes were positive for TrkB immunoreactivity. Furthermore, biotinylation and subsynaptic fractionation studies showed that only one-fourth of total full-length TrkB was present at the plasma membrane, evenly distributed between the presynaptic active zone and the postsynaptic density. These results indicate that BDNF modulates synaptic transmission presynaptically in a small subset of hippocampal glutamatergic synapses that contain TrkB and that express the receptor on the plasma membrane. © 2006 Wiley-Liss, Inc.
publishDate 2006
dc.date.none.fl_str_mv 2006
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/8320
http://hdl.handle.net/10316/8320
https://doi.org/10.1002/jnr.20784
url http://hdl.handle.net/10316/8320
https://doi.org/10.1002/jnr.20784
dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv Journal of Neuroscience Research. 83:5 (2006) 832-844
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