Dried blood spots in clinical lipidomics: optimization and recent findings
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.18/8533 |
Resumo: | Review |
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Dried blood spots in clinical lipidomics: optimization and recent findingsDisease BiomarkersDried Blood SpotsLipidomicsMass SpectrometryDoenças GenéticasReviewDried blood spots (DBS) are being considered as an alternative sampling method of blood collection that can be used in combination with lipidomic and other omic analysis. DBS are successfully used in the clinical context to collect samples for newborn screening for the measurement of specific fatty acid derivatives, such as acylcarnitines, and lipids from whole blood for diagnostic purposes. However, DBS are scarcely used for lipidomic analysis and investigations. Lipidomic studies using DBS are starting to emerge as a powerful method for sampling and storage in clinical lipidomic analysis, but the major research work is being done in the pre- and analytical steps and procedures, and few in clinical applications. This review presents a description of the impact factors and variables that can affect DBS lipidomic analysis, such as the type of DBS card, haematocrit, homogeneity of the blood drop, matrix/chromatographic effects, and the chemical and physical properties of the analyte. Additionally, a brief overview of lipidomic studies using DBS to unveil their application in clinical scenarios is also presented, considering the studies of method development and validation and, to a less extent, for clinical diagnosis using clinical lipidomics. DBS combined with lipidomic approaches proved to be as effective as whole blood samples, achieving high levels of sensitivity and specificity during MS and MS/MS analysis, which could be a useful tool for biomarker identification. Lipidomic profiling using MS/MS platforms enables significant insights into physiological changes, which could be useful in precision medicine.Thanks are due for the financial support to the University of Aveiro and FCT/MCT for the fnancial support to research units CESAM (UIDB/50017/2020, UIDP/50017/2020 & LA/P/0094/2020), LAQV-REQUIMTE (UIDB/50006/2020), and CICECO-Aveiro Institute of Materials (UIDB/50011/2020, UIDP/50011/2020 & LA/P/0006/2020), and to RNEM, Portuguese Mass Spectrometry Network (LISBOA-01-0145-FEDER-402-022125) through national funds and, where applicable, co-fnanced by the FEDER, within the PT2020. Helena Beatriz Ferreira is grateful to FCT for her PhD Grant (2020.04611.BD). Inês M. S. Guerra is grateful to FCT for her PhD Grant (2021.04754.BD). Ana S. P. Moreira thanks the contract in the scope of the project ‘Coccolitho4BioMat’ (POCI 01-0145-FEDER-031032). Tânia Melo thanks the Junior Researcher contract in the scope of the Individual Call to Scientifc Employment Stimulus 2020 (CEECIND/01578/2020). The authors are thankful to the COST Action EpiLipidNET, CA19105-Pan-European Network in Lipidomics and EpiLipidomics.SpringerRepositório Científico do Instituto Nacional de SaúdeFerreira, Helena BeatrizGuerra, Inês M.S.Melo, TâniaRocha, HugoMoreira, Ana S.P.Paiva, ArturDomingues, M. Rosário2023-02-28T11:45:55Z2022-102022-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8533engAnal Bioanal Chem. 2022 Oct;414(24):7085-7101. doi: 10.1007/s00216-022-04221-1. Epub 2022 Jul 15. Review1618-264210.1007/s00216-022-04221-1info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:37Zoai:repositorio.insa.pt:10400.18/8533Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:43:10.576071Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Dried blood spots in clinical lipidomics: optimization and recent findings |
| title |
Dried blood spots in clinical lipidomics: optimization and recent findings |
| spellingShingle |
Dried blood spots in clinical lipidomics: optimization and recent findings Ferreira, Helena Beatriz Disease Biomarkers Dried Blood Spots Lipidomics Mass Spectrometry Doenças Genéticas |
| title_short |
Dried blood spots in clinical lipidomics: optimization and recent findings |
| title_full |
Dried blood spots in clinical lipidomics: optimization and recent findings |
| title_fullStr |
Dried blood spots in clinical lipidomics: optimization and recent findings |
| title_full_unstemmed |
Dried blood spots in clinical lipidomics: optimization and recent findings |
| title_sort |
Dried blood spots in clinical lipidomics: optimization and recent findings |
| author |
Ferreira, Helena Beatriz |
| author_facet |
Ferreira, Helena Beatriz Guerra, Inês M.S. Melo, Tânia Rocha, Hugo Moreira, Ana S.P. Paiva, Artur Domingues, M. Rosário |
| author_role |
author |
| author2 |
Guerra, Inês M.S. Melo, Tânia Rocha, Hugo Moreira, Ana S.P. Paiva, Artur Domingues, M. Rosário |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
| dc.contributor.author.fl_str_mv |
Ferreira, Helena Beatriz Guerra, Inês M.S. Melo, Tânia Rocha, Hugo Moreira, Ana S.P. Paiva, Artur Domingues, M. Rosário |
| dc.subject.por.fl_str_mv |
Disease Biomarkers Dried Blood Spots Lipidomics Mass Spectrometry Doenças Genéticas |
| topic |
Disease Biomarkers Dried Blood Spots Lipidomics Mass Spectrometry Doenças Genéticas |
| description |
Review |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-10 2022-10-01T00:00:00Z 2023-02-28T11:45:55Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/8533 |
| url |
http://hdl.handle.net/10400.18/8533 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Anal Bioanal Chem. 2022 Oct;414(24):7085-7101. doi: 10.1007/s00216-022-04221-1. Epub 2022 Jul 15. Review 1618-2642 10.1007/s00216-022-04221-1 |
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info:eu-repo/semantics/embargoedAccess |
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embargoedAccess |
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application/pdf |
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Springer |
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Springer |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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