Fructophilic behaviour of Gordonia alkanivorans strain 1B during dibenzothiophene desulfurization process

Detalhes bibliográficos
Autor(a) principal: Alves, Luís
Data de Publicação: 2014
Outros Autores: Paixão, Susana M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.9/2259
Resumo: Biodesulfurization (BDS) aims at the removal of recalcitrant sulfur from fossil fuels at mild operating conditions with the aid of microorganisms. These microorganisms can remove sulfur from dibenzothiphene (DBT), a model compound, or other polycyclic aromatic used as sulfur source, making BDS an easy and environmental friendly process. Gordonia alkanivorans strain 1B has been described as a desulfurizing bacterium, able to desulfurize DBT to 2-hydroxybiphenyl (2-HBP), the final product of the 4S pathway, using d-glucose as carbon source. However, both cell growth and desulfurization can be largely affected by the nutrient composition of the growth medium, due to cofactor requirements of many enzymes involved in the BDS biochemical pathway. In this study, the main goal was to investigate the influence of several sugars, as carbon source, on the growth and DBT desulfurization ability of G. alkanivorans strain 1B. The results of desulfurization tests showed that the lowest values for the growth rate (0.025 hour-1) and for the overall 2-HBP production rate (1.80 µm/hour) by the strain 1B were obtained in glucose grown cultures. When using sucrose, the growth rate increase exhibited by strain 1B led to a higher biomass productivity, which induced a slightly increase in the 2-HBP production rate (1.91 µm/hour), conversely in terms of 2-HBP specific production rate (q2-HBP) the value obtained was markedly lower (0.718 µmol/g/hour in sucrose versus 1.22 µmol/g/hour in glucose). When a mixture of glucose and fructose was used as carbon source, strain 1B reached a value of q2-HBP = 1.90 µmol/g/hour, close to that in fructose (q2-HBP = 2.12 µmol/g/hour). The highest values for both cell growth (µ = 0.091 hour-1) and 2-HPB production (9.29 µm/hour) were obtained when strain 1B was desulfurizing DBT in the presence of fructose as the only carbon source, indicating a fructophilic behaviour by this bacterium. This fact is in agreement with the highest value of biomass productivity by strain 1B be in fructose, which resulted in a higher amount cells fulfilling the DBT-desulfurization. The greater number of functional cells conducted to a more effectiveness BDS process by strain 1B, as they attained a q2-HBP about 74% higher than in glucose grown cultures. Moreover, this significant BDS enhancement can better be observed in terms of the overall 2-HBP production rate, which increased over 5-fold, from 1.80 µm/hour (in glucose) to 9.
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spelling Fructophilic behaviour of Gordonia alkanivorans strain 1B during dibenzothiophene desulfurization processGordonia alkanivoransDibenzothiopheneBiodesulfurizationBiodesulfurization (BDS) aims at the removal of recalcitrant sulfur from fossil fuels at mild operating conditions with the aid of microorganisms. These microorganisms can remove sulfur from dibenzothiphene (DBT), a model compound, or other polycyclic aromatic used as sulfur source, making BDS an easy and environmental friendly process. Gordonia alkanivorans strain 1B has been described as a desulfurizing bacterium, able to desulfurize DBT to 2-hydroxybiphenyl (2-HBP), the final product of the 4S pathway, using d-glucose as carbon source. However, both cell growth and desulfurization can be largely affected by the nutrient composition of the growth medium, due to cofactor requirements of many enzymes involved in the BDS biochemical pathway. In this study, the main goal was to investigate the influence of several sugars, as carbon source, on the growth and DBT desulfurization ability of G. alkanivorans strain 1B. The results of desulfurization tests showed that the lowest values for the growth rate (0.025 hour-1) and for the overall 2-HBP production rate (1.80 µm/hour) by the strain 1B were obtained in glucose grown cultures. When using sucrose, the growth rate increase exhibited by strain 1B led to a higher biomass productivity, which induced a slightly increase in the 2-HBP production rate (1.91 µm/hour), conversely in terms of 2-HBP specific production rate (q2-HBP) the value obtained was markedly lower (0.718 µmol/g/hour in sucrose versus 1.22 µmol/g/hour in glucose). When a mixture of glucose and fructose was used as carbon source, strain 1B reached a value of q2-HBP = 1.90 µmol/g/hour, close to that in fructose (q2-HBP = 2.12 µmol/g/hour). The highest values for both cell growth (µ = 0.091 hour-1) and 2-HPB production (9.29 µm/hour) were obtained when strain 1B was desulfurizing DBT in the presence of fructose as the only carbon source, indicating a fructophilic behaviour by this bacterium. This fact is in agreement with the highest value of biomass productivity by strain 1B be in fructose, which resulted in a higher amount cells fulfilling the DBT-desulfurization. The greater number of functional cells conducted to a more effectiveness BDS process by strain 1B, as they attained a q2-HBP about 74% higher than in glucose grown cultures. Moreover, this significant BDS enhancement can better be observed in terms of the overall 2-HBP production rate, which increased over 5-fold, from 1.80 µm/hour (in glucose) to 9.ElsevierRepositório do LNEGAlves, LuísPaixão, Susana M.2014-03-04T17:08:05Z20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.9/2259engAlves, Luis; Paixão, Susana M. Fructophilic behaviour of Gordonia alkanivorans strain 1B during dibenzothiophene desulfurization process. In: New Biotechnology, 2014, Vol. 31, nº 1, p. 73-791871-6784info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-25T11:17:00Zoai:repositorio.lneg.pt:10400.9/2259Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:35:08.745042Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Fructophilic behaviour of Gordonia alkanivorans strain 1B during dibenzothiophene desulfurization process
title Fructophilic behaviour of Gordonia alkanivorans strain 1B during dibenzothiophene desulfurization process
spellingShingle Fructophilic behaviour of Gordonia alkanivorans strain 1B during dibenzothiophene desulfurization process
Alves, Luís
Gordonia alkanivorans
Dibenzothiophene
Biodesulfurization
title_short Fructophilic behaviour of Gordonia alkanivorans strain 1B during dibenzothiophene desulfurization process
title_full Fructophilic behaviour of Gordonia alkanivorans strain 1B during dibenzothiophene desulfurization process
title_fullStr Fructophilic behaviour of Gordonia alkanivorans strain 1B during dibenzothiophene desulfurization process
title_full_unstemmed Fructophilic behaviour of Gordonia alkanivorans strain 1B during dibenzothiophene desulfurization process
title_sort Fructophilic behaviour of Gordonia alkanivorans strain 1B during dibenzothiophene desulfurization process
author Alves, Luís
author_facet Alves, Luís
Paixão, Susana M.
author_role author
author2 Paixão, Susana M.
author2_role author
dc.contributor.none.fl_str_mv Repositório do LNEG
dc.contributor.author.fl_str_mv Alves, Luís
Paixão, Susana M.
dc.subject.por.fl_str_mv Gordonia alkanivorans
Dibenzothiophene
Biodesulfurization
topic Gordonia alkanivorans
Dibenzothiophene
Biodesulfurization
description Biodesulfurization (BDS) aims at the removal of recalcitrant sulfur from fossil fuels at mild operating conditions with the aid of microorganisms. These microorganisms can remove sulfur from dibenzothiphene (DBT), a model compound, or other polycyclic aromatic used as sulfur source, making BDS an easy and environmental friendly process. Gordonia alkanivorans strain 1B has been described as a desulfurizing bacterium, able to desulfurize DBT to 2-hydroxybiphenyl (2-HBP), the final product of the 4S pathway, using d-glucose as carbon source. However, both cell growth and desulfurization can be largely affected by the nutrient composition of the growth medium, due to cofactor requirements of many enzymes involved in the BDS biochemical pathway. In this study, the main goal was to investigate the influence of several sugars, as carbon source, on the growth and DBT desulfurization ability of G. alkanivorans strain 1B. The results of desulfurization tests showed that the lowest values for the growth rate (0.025 hour-1) and for the overall 2-HBP production rate (1.80 µm/hour) by the strain 1B were obtained in glucose grown cultures. When using sucrose, the growth rate increase exhibited by strain 1B led to a higher biomass productivity, which induced a slightly increase in the 2-HBP production rate (1.91 µm/hour), conversely in terms of 2-HBP specific production rate (q2-HBP) the value obtained was markedly lower (0.718 µmol/g/hour in sucrose versus 1.22 µmol/g/hour in glucose). When a mixture of glucose and fructose was used as carbon source, strain 1B reached a value of q2-HBP = 1.90 µmol/g/hour, close to that in fructose (q2-HBP = 2.12 µmol/g/hour). The highest values for both cell growth (µ = 0.091 hour-1) and 2-HPB production (9.29 µm/hour) were obtained when strain 1B was desulfurizing DBT in the presence of fructose as the only carbon source, indicating a fructophilic behaviour by this bacterium. This fact is in agreement with the highest value of biomass productivity by strain 1B be in fructose, which resulted in a higher amount cells fulfilling the DBT-desulfurization. The greater number of functional cells conducted to a more effectiveness BDS process by strain 1B, as they attained a q2-HBP about 74% higher than in glucose grown cultures. Moreover, this significant BDS enhancement can better be observed in terms of the overall 2-HBP production rate, which increased over 5-fold, from 1.80 µm/hour (in glucose) to 9.
publishDate 2014
dc.date.none.fl_str_mv 2014-03-04T17:08:05Z
2014
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.9/2259
url http://hdl.handle.net/10400.9/2259
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Alves, Luis; Paixão, Susana M. Fructophilic behaviour of Gordonia alkanivorans strain 1B during dibenzothiophene desulfurization process. In: New Biotechnology, 2014, Vol. 31, nº 1, p. 73-79
1871-6784
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
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