Protein Imprinted Material electrochemical sensor for determination of Annexin A3 in biological samples
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/10039 |
Resumo: | The development of fast and reliable methods for protein determination are of great relevance to a diversity of areas from industry to diagnostics. Molecular Imprinted Materials (MIM) has proved to be an interesting methodology for protein analysis however further studies of the effect of the experimental parameters and starting materials in the performance of the MIM are still required. Caffeic acid (CAF) is employed for the first time as a monomer to tailor a synthetic receptor for a protein target. This was done by bulk-electropolymerization, applying a constant potential of +2.0 V, for 30 s, on a carbon screen- printed electrode, immersed in a solution of protein and CAF prepared in phosphate buffer. Annexin A3 (ANXA3) was selected as protein target due to the fact that this is an emerging biomarker in prostate cancer. The assembly of the protein imprinted material (PIM) was followed by Electrochemical Impedance Spectroscopy (EIS) and Raman Spectroscopy. A non-imprinted material (NIM) was prepared in parallel as control. Square wave voltammetry (SWV) was used to monitor the electrochemical signal of the [Fe(CN)6]3 /[Fe (CN)6]4 redox for the quantification of ANXA3. The optimized PIM-based device showed average detection limits (LOD) of 0.095 ng/mL, a linear behavior against log (concentration) between 0.10, and 200 ng/mL and good selectivity. The NIM-based device showed random behavior against protein concentration. Finally, the PIM-sensor was successfully applied to the analysis of ANXA3 in spiked urine samples. |
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Protein Imprinted Material electrochemical sensor for determination of Annexin A3 in biological samplesAnnexin A3Caffeic acidElectrochemical biosensorProtein imprinted materialScreen-printed electrodesUrineThe development of fast and reliable methods for protein determination are of great relevance to a diversity of areas from industry to diagnostics. Molecular Imprinted Materials (MIM) has proved to be an interesting methodology for protein analysis however further studies of the effect of the experimental parameters and starting materials in the performance of the MIM are still required. Caffeic acid (CAF) is employed for the first time as a monomer to tailor a synthetic receptor for a protein target. This was done by bulk-electropolymerization, applying a constant potential of +2.0 V, for 30 s, on a carbon screen- printed electrode, immersed in a solution of protein and CAF prepared in phosphate buffer. Annexin A3 (ANXA3) was selected as protein target due to the fact that this is an emerging biomarker in prostate cancer. The assembly of the protein imprinted material (PIM) was followed by Electrochemical Impedance Spectroscopy (EIS) and Raman Spectroscopy. A non-imprinted material (NIM) was prepared in parallel as control. Square wave voltammetry (SWV) was used to monitor the electrochemical signal of the [Fe(CN)6]3 /[Fe (CN)6]4 redox for the quantification of ANXA3. The optimized PIM-based device showed average detection limits (LOD) of 0.095 ng/mL, a linear behavior against log (concentration) between 0.10, and 200 ng/mL and good selectivity. The NIM-based device showed random behavior against protein concentration. Finally, the PIM-sensor was successfully applied to the analysis of ANXA3 in spiked urine samples.ElsevierRepositório Científico do Instituto Politécnico do PortoRebelo, Tânia S.C.R.Pereira, Carlos M.Sales, GoretiNoronha, João P.Silva, Fernando20162117-01-01T00:00:00Z2016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/10039eng10.1016/j.electacta.2015.12.214metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:51:32Zoai:recipp.ipp.pt:10400.22/10039Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:30:30.748942Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Protein Imprinted Material electrochemical sensor for determination of Annexin A3 in biological samples |
title |
Protein Imprinted Material electrochemical sensor for determination of Annexin A3 in biological samples |
spellingShingle |
Protein Imprinted Material electrochemical sensor for determination of Annexin A3 in biological samples Rebelo, Tânia S.C.R. Annexin A3 Caffeic acid Electrochemical biosensor Protein imprinted material Screen-printed electrodes Urine |
title_short |
Protein Imprinted Material electrochemical sensor for determination of Annexin A3 in biological samples |
title_full |
Protein Imprinted Material electrochemical sensor for determination of Annexin A3 in biological samples |
title_fullStr |
Protein Imprinted Material electrochemical sensor for determination of Annexin A3 in biological samples |
title_full_unstemmed |
Protein Imprinted Material electrochemical sensor for determination of Annexin A3 in biological samples |
title_sort |
Protein Imprinted Material electrochemical sensor for determination of Annexin A3 in biological samples |
author |
Rebelo, Tânia S.C.R. |
author_facet |
Rebelo, Tânia S.C.R. Pereira, Carlos M. Sales, Goreti Noronha, João P. Silva, Fernando |
author_role |
author |
author2 |
Pereira, Carlos M. Sales, Goreti Noronha, João P. Silva, Fernando |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Rebelo, Tânia S.C.R. Pereira, Carlos M. Sales, Goreti Noronha, João P. Silva, Fernando |
dc.subject.por.fl_str_mv |
Annexin A3 Caffeic acid Electrochemical biosensor Protein imprinted material Screen-printed electrodes Urine |
topic |
Annexin A3 Caffeic acid Electrochemical biosensor Protein imprinted material Screen-printed electrodes Urine |
description |
The development of fast and reliable methods for protein determination are of great relevance to a diversity of areas from industry to diagnostics. Molecular Imprinted Materials (MIM) has proved to be an interesting methodology for protein analysis however further studies of the effect of the experimental parameters and starting materials in the performance of the MIM are still required. Caffeic acid (CAF) is employed for the first time as a monomer to tailor a synthetic receptor for a protein target. This was done by bulk-electropolymerization, applying a constant potential of +2.0 V, for 30 s, on a carbon screen- printed electrode, immersed in a solution of protein and CAF prepared in phosphate buffer. Annexin A3 (ANXA3) was selected as protein target due to the fact that this is an emerging biomarker in prostate cancer. The assembly of the protein imprinted material (PIM) was followed by Electrochemical Impedance Spectroscopy (EIS) and Raman Spectroscopy. A non-imprinted material (NIM) was prepared in parallel as control. Square wave voltammetry (SWV) was used to monitor the electrochemical signal of the [Fe(CN)6]3 /[Fe (CN)6]4 redox for the quantification of ANXA3. The optimized PIM-based device showed average detection limits (LOD) of 0.095 ng/mL, a linear behavior against log (concentration) between 0.10, and 200 ng/mL and good selectivity. The NIM-based device showed random behavior against protein concentration. Finally, the PIM-sensor was successfully applied to the analysis of ANXA3 in spiked urine samples. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2016-01-01T00:00:00Z 2117-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/10039 |
url |
http://hdl.handle.net/10400.22/10039 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.electacta.2015.12.214 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131400718254080 |