Modulation of the mesenchymal stem cell secretome using computer-controlled bioreactors: impact on neuronal cell proliferation, survival and differentiation
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/50239 |
Resumo: | In recent years it has been shown that the therapeutic benefits of human mesenchymal stem/ stromal cells (hMSCs) in the Central Nervous System (CNS) are mainly attributed to their secretome. The implementation of computer-controlled suspension bioreactors has shown to be a viable route for the expansion of these cells to large numbers. As hMSCs actively respond to their culture environment, there is the hypothesis that one can modulate its secretome through their use. Herein, we present data indicating that the use of computer-controlled suspension bioreactors enhanced the neuroregulatory profile of hMSCs secretome. Indeed, higher levels of in vitro neuronal differentiation and NOTCH1 expression in human neural progenitor cells (hNPCs) were observed when these cells were incubated with the secretome of dynamically cultured hMSCs. A similar trend was also observed in the hippocampal dentate gyrus (DG) of rat brains where, upon injection, an enhanced neuronal and astrocytic survival and differentiation, was observed. Proteomic analysis also revealed that the dynamic culturing of hMSCs increased the secretion of several neuroregulatory molecules and miRNAs present in hMSCs secretome. In summary, the appropriate use of dynamic culture conditions can represent an important asset for the development of future neuro-regenerative strategies involving the use of hMSCs secretome. |
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Modulation of the mesenchymal stem cell secretome using computer-controlled bioreactors: impact on neuronal cell proliferation, survival and differentiation2016 Jun 15Science & TechnologyIn recent years it has been shown that the therapeutic benefits of human mesenchymal stem/ stromal cells (hMSCs) in the Central Nervous System (CNS) are mainly attributed to their secretome. The implementation of computer-controlled suspension bioreactors has shown to be a viable route for the expansion of these cells to large numbers. As hMSCs actively respond to their culture environment, there is the hypothesis that one can modulate its secretome through their use. Herein, we present data indicating that the use of computer-controlled suspension bioreactors enhanced the neuroregulatory profile of hMSCs secretome. Indeed, higher levels of in vitro neuronal differentiation and NOTCH1 expression in human neural progenitor cells (hNPCs) were observed when these cells were incubated with the secretome of dynamically cultured hMSCs. A similar trend was also observed in the hippocampal dentate gyrus (DG) of rat brains where, upon injection, an enhanced neuronal and astrocytic survival and differentiation, was observed. Proteomic analysis also revealed that the dynamic culturing of hMSCs increased the secretion of several neuroregulatory molecules and miRNAs present in hMSCs secretome. In summary, the appropriate use of dynamic culture conditions can represent an important asset for the development of future neuro-regenerative strategies involving the use of hMSCs secretome.We acknowledge the financial support of the Portuguese Foundation for Science and Technology (FCT) for a Ciencia 2007 program and IF Development Grant (AJS), a pre-doctoral fellowship to FGT (SFRH/69637/2010) and a PhD fellowship to SA (SFRH/BD/81495/2011), a Canada Research Chair in Biomedical Engineering (LAB) and a SSE Postdoctoral Fellowship (KMP). Premios Santa Casa Neurociencias - Prize Melo e Castro for Spinal Cord Injury Research. PEst-C/SAU/LA0001/2013-2014. Co-funded by "COMPETE Programa Operacional Factores de Competitividade", QREN, the European Union (FEDER - Fundo Europeu de Desenvolvimento Regional) and by The National Mass Spectrometry Network (RNEM) under the contract REDE/1506/REM/2005.info:eu-repo/semantics/publishedVersionSpringer NatureUniversidade do MinhoTeixeira, Fábio Gabriel RodriguesPanchalingam, Krishna M.Silva, Rita Catarina Assunção RibeiroSerra, Sofia CravinoPinheiro, Bárbara Filipa MendesPatrício, P.Jung, SunghoonAnjo, Sandra I.Manadas, BrunoPinto, LuísaSousa, NunoBehie, Leo A.Salgado, A. J.2016-062016-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/50239eng2045-23222045-232210.1038/srep2779127301770https://www.nature.com/srep/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:32:46Zoai:repositorium.sdum.uminho.pt:1822/50239Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:28:09.556569Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Modulation of the mesenchymal stem cell secretome using computer-controlled bioreactors: impact on neuronal cell proliferation, survival and differentiation 2016 Jun 15 |
title |
Modulation of the mesenchymal stem cell secretome using computer-controlled bioreactors: impact on neuronal cell proliferation, survival and differentiation |
spellingShingle |
Modulation of the mesenchymal stem cell secretome using computer-controlled bioreactors: impact on neuronal cell proliferation, survival and differentiation Teixeira, Fábio Gabriel Rodrigues Science & Technology |
title_short |
Modulation of the mesenchymal stem cell secretome using computer-controlled bioreactors: impact on neuronal cell proliferation, survival and differentiation |
title_full |
Modulation of the mesenchymal stem cell secretome using computer-controlled bioreactors: impact on neuronal cell proliferation, survival and differentiation |
title_fullStr |
Modulation of the mesenchymal stem cell secretome using computer-controlled bioreactors: impact on neuronal cell proliferation, survival and differentiation |
title_full_unstemmed |
Modulation of the mesenchymal stem cell secretome using computer-controlled bioreactors: impact on neuronal cell proliferation, survival and differentiation |
title_sort |
Modulation of the mesenchymal stem cell secretome using computer-controlled bioreactors: impact on neuronal cell proliferation, survival and differentiation |
author |
Teixeira, Fábio Gabriel Rodrigues |
author_facet |
Teixeira, Fábio Gabriel Rodrigues Panchalingam, Krishna M. Silva, Rita Catarina Assunção Ribeiro Serra, Sofia Cravino Pinheiro, Bárbara Filipa Mendes Patrício, P. Jung, Sunghoon Anjo, Sandra I. Manadas, Bruno Pinto, Luísa Sousa, Nuno Behie, Leo A. Salgado, A. J. |
author_role |
author |
author2 |
Panchalingam, Krishna M. Silva, Rita Catarina Assunção Ribeiro Serra, Sofia Cravino Pinheiro, Bárbara Filipa Mendes Patrício, P. Jung, Sunghoon Anjo, Sandra I. Manadas, Bruno Pinto, Luísa Sousa, Nuno Behie, Leo A. Salgado, A. J. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Teixeira, Fábio Gabriel Rodrigues Panchalingam, Krishna M. Silva, Rita Catarina Assunção Ribeiro Serra, Sofia Cravino Pinheiro, Bárbara Filipa Mendes Patrício, P. Jung, Sunghoon Anjo, Sandra I. Manadas, Bruno Pinto, Luísa Sousa, Nuno Behie, Leo A. Salgado, A. J. |
dc.subject.por.fl_str_mv |
Science & Technology |
topic |
Science & Technology |
description |
In recent years it has been shown that the therapeutic benefits of human mesenchymal stem/ stromal cells (hMSCs) in the Central Nervous System (CNS) are mainly attributed to their secretome. The implementation of computer-controlled suspension bioreactors has shown to be a viable route for the expansion of these cells to large numbers. As hMSCs actively respond to their culture environment, there is the hypothesis that one can modulate its secretome through their use. Herein, we present data indicating that the use of computer-controlled suspension bioreactors enhanced the neuroregulatory profile of hMSCs secretome. Indeed, higher levels of in vitro neuronal differentiation and NOTCH1 expression in human neural progenitor cells (hNPCs) were observed when these cells were incubated with the secretome of dynamically cultured hMSCs. A similar trend was also observed in the hippocampal dentate gyrus (DG) of rat brains where, upon injection, an enhanced neuronal and astrocytic survival and differentiation, was observed. Proteomic analysis also revealed that the dynamic culturing of hMSCs increased the secretion of several neuroregulatory molecules and miRNAs present in hMSCs secretome. In summary, the appropriate use of dynamic culture conditions can represent an important asset for the development of future neuro-regenerative strategies involving the use of hMSCs secretome. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06 2016-06-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/50239 |
url |
http://hdl.handle.net/1822/50239 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2045-2322 2045-2322 10.1038/srep27791 27301770 https://www.nature.com/srep/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer Nature |
publisher.none.fl_str_mv |
Springer Nature |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132776572649472 |