Experimental design and sample preparation in forest tree matabolomics

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Ana M.
Data de Publicação: 2019
Outros Autores: Ribeiro-Barros, Ana I., António, Carla
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/19027
Resumo: Review
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spelling Experimental design and sample preparation in forest tree matabolomicsplant metabolomicsforestrytreesmass spectrometrymetabolite extractionGC-MSLC-MSmetadata standardizationdatabasesReviewAppropriate experimental design and sample preparation are key steps in metabolomics experiments, highly influencing the biological interpretation of the results. The sample preparation workflow for plant metabolomics studies includes several steps before metabolite extraction and analysis. These include the optimization of laboratory procedures, which should be optimized for di erent plants and tissues. This is particularly the case for trees, whose tissues are complex matrices to work with due to the presence of several interferents, such as oleoresins, cellulose. A good experimental design, tree tissue harvest conditions, and sample preparation are crucial to ensure consistency and reproducibility of the metadata among datasets. In this review, we discuss the main challenges when setting up a forest tree metabolomics experiment for mass spectrometry (MS)-based analysis covering all technical aspects from the biological question formulation and experimental design to sample processing and metabolite extraction and data acquisition. We also highlight the importance of forest tree metadata standardization in metabolomics studiesMDPIRepositório da Universidade de LisboaRodrigues, Ana M.Ribeiro-Barros, Ana I.António, Carla2019-12-12T14:48:24Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/19027engMetabolites 2019, 9, 28510.3390/metabo9120285info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:48:33Zoai:www.repository.utl.pt:10400.5/19027Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:03:58.902091Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Experimental design and sample preparation in forest tree matabolomics
title Experimental design and sample preparation in forest tree matabolomics
spellingShingle Experimental design and sample preparation in forest tree matabolomics
Rodrigues, Ana M.
plant metabolomics
forestry
trees
mass spectrometry
metabolite extraction
GC-MS
LC-MS
metadata standardization
databases
title_short Experimental design and sample preparation in forest tree matabolomics
title_full Experimental design and sample preparation in forest tree matabolomics
title_fullStr Experimental design and sample preparation in forest tree matabolomics
title_full_unstemmed Experimental design and sample preparation in forest tree matabolomics
title_sort Experimental design and sample preparation in forest tree matabolomics
author Rodrigues, Ana M.
author_facet Rodrigues, Ana M.
Ribeiro-Barros, Ana I.
António, Carla
author_role author
author2 Ribeiro-Barros, Ana I.
António, Carla
author2_role author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Rodrigues, Ana M.
Ribeiro-Barros, Ana I.
António, Carla
dc.subject.por.fl_str_mv plant metabolomics
forestry
trees
mass spectrometry
metabolite extraction
GC-MS
LC-MS
metadata standardization
databases
topic plant metabolomics
forestry
trees
mass spectrometry
metabolite extraction
GC-MS
LC-MS
metadata standardization
databases
description Review
publishDate 2019
dc.date.none.fl_str_mv 2019-12-12T14:48:24Z
2019
2019-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/19027
url http://hdl.handle.net/10400.5/19027
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Metabolites 2019, 9, 285
10.3390/metabo9120285
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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