Evaluation of two laboratory methods for the diagnosis of hydatidosis

Detalhes bibliográficos
Autor(a) principal: Batista, Generosa
Data de Publicação: 2009
Outros Autores: Magalhães, Zúzeca, Abreu, Renato, Caria, Elisa, Almeida, Ana
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.25758/set.204
Resumo: Hydatidosis, also known as hydatid cyst, is caused by the larval stages of the parasite Echinococcus granulosus. Its diagnostic relies mainly on clinical findings, epidemiology, and imaging techniques supported by serological tests. Surgery is the commonest treatment; it is important to have a definitive diagnosis of hydatidosis before surgery, to avoid the spread of the cysts during the procedure. In this study, two immunoserologic tests for the diagnostic of hydatidosis are compared: Enzyme-linked Immunosorbent Assay (ELISA) for immunoglobulin G; and Fluoro-Enzyme Immunoassay (FEIA) for immunoglobulin E. Of the 55 patients included in this study are in a pre-surgery stage, 31% have a calcified hydatic cyst, 54,5% a not calcified cyst and 14.5% have no hydatic cyst. Both tests had the same specificity (87.5%), and the sensitivity of the ELISA IgG was lower (63.8%) than the FEIA IgE (76.6%). The correlation observed between both tests was good (r = 0.726, p < 0.05).
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spelling Evaluation of two laboratory methods for the diagnosis of hydatidosisAvaliação de dois métodos laboratoriais para diagnóstico de hidatidoseEchinococcus granulosusHidatidoseELISAFEIAEchinococcus granulosusHydatidosisELISAFEIAHydatidosis, also known as hydatid cyst, is caused by the larval stages of the parasite Echinococcus granulosus. Its diagnostic relies mainly on clinical findings, epidemiology, and imaging techniques supported by serological tests. Surgery is the commonest treatment; it is important to have a definitive diagnosis of hydatidosis before surgery, to avoid the spread of the cysts during the procedure. In this study, two immunoserologic tests for the diagnostic of hydatidosis are compared: Enzyme-linked Immunosorbent Assay (ELISA) for immunoglobulin G; and Fluoro-Enzyme Immunoassay (FEIA) for immunoglobulin E. Of the 55 patients included in this study are in a pre-surgery stage, 31% have a calcified hydatic cyst, 54,5% a not calcified cyst and 14.5% have no hydatic cyst. Both tests had the same specificity (87.5%), and the sensitivity of the ELISA IgG was lower (63.8%) than the FEIA IgE (76.6%). The correlation observed between both tests was good (r = 0.726, p < 0.05).A hidatidose, vulgarmente conhecida por quisto hidático, é causada pelos estados larvares do parasita Echinococcus granulosus. O seu diagnóstico baseia-se na clínica, na epidemiologia e nas técnicas imagiológicas, sendo suportado por testes serológicos. O tratamento mais comum é o cirúrgico, sendo importante o diagnóstico definitivo da doença antes da cirurgia, para se prevenir a disseminação dos quistos que pode ocorrer durante a mesma. Neste trabalho comparam-se dois métodos imunoserológicos para odiagnóstico da hidatidose: Enzyme-linked Immunosorbent Assay (ELISA) para pesquisa de Imunoglobulina G; e Fluoro-enzyme Immunoassay (FEIA) para pesquisa de Imunoglobulina E. Dos 55 indivíduos incluídos no estudo, todos em fase pré-operatória, 31% possuíam quisto hidático calcificado, 54,5% quisto hidático não calcificado e 14,5% não possuíam hidatidose, mas quistos simples.  Os testes apresentaram a mesma especificidade (87,5%), sendo a sensibilidade do ELISA IgG mais baixa (63,8%) do que a do FEIA IgE (76,6%). Foi demonstrada uma boa correlação entre os dois métodos (r = 0,726, p < 0,05). Escola Superior de Tecnologia da Saúde de Lisboa (Instituto Politécnico de Lisboa)2009-05-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.25758/set.204https://doi.org/10.25758/set.204Saúde e Tecnologia; No. 03 (2009): Maio 2009; 15-18Saúde & Tecnologia; N.º 03 (2009): Maio 2009; 15-181646-9704reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPporhttps://journals.ipl.pt/stecnologia/article/view/753https://journals.ipl.pt/stecnologia/article/view/753/642Direitos de Autor (c) 2023 Saúde & Tecnologiainfo:eu-repo/semantics/openAccessBatista, GenerosaMagalhães, ZúzecaAbreu, RenatoCaria, ElisaAlmeida, Ana2023-05-12T08:30:24Zoai:journals.ipl.pt:article/753Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:42:45.846067Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Evaluation of two laboratory methods for the diagnosis of hydatidosis
Avaliação de dois métodos laboratoriais para diagnóstico de hidatidose
title Evaluation of two laboratory methods for the diagnosis of hydatidosis
spellingShingle Evaluation of two laboratory methods for the diagnosis of hydatidosis
Batista, Generosa
Echinococcus granulosus
Hidatidose
ELISA
FEIA
Echinococcus granulosus
Hydatidosis
ELISA
FEIA
title_short Evaluation of two laboratory methods for the diagnosis of hydatidosis
title_full Evaluation of two laboratory methods for the diagnosis of hydatidosis
title_fullStr Evaluation of two laboratory methods for the diagnosis of hydatidosis
title_full_unstemmed Evaluation of two laboratory methods for the diagnosis of hydatidosis
title_sort Evaluation of two laboratory methods for the diagnosis of hydatidosis
author Batista, Generosa
author_facet Batista, Generosa
Magalhães, Zúzeca
Abreu, Renato
Caria, Elisa
Almeida, Ana
author_role author
author2 Magalhães, Zúzeca
Abreu, Renato
Caria, Elisa
Almeida, Ana
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Batista, Generosa
Magalhães, Zúzeca
Abreu, Renato
Caria, Elisa
Almeida, Ana
dc.subject.por.fl_str_mv Echinococcus granulosus
Hidatidose
ELISA
FEIA
Echinococcus granulosus
Hydatidosis
ELISA
FEIA
topic Echinococcus granulosus
Hidatidose
ELISA
FEIA
Echinococcus granulosus
Hydatidosis
ELISA
FEIA
description Hydatidosis, also known as hydatid cyst, is caused by the larval stages of the parasite Echinococcus granulosus. Its diagnostic relies mainly on clinical findings, epidemiology, and imaging techniques supported by serological tests. Surgery is the commonest treatment; it is important to have a definitive diagnosis of hydatidosis before surgery, to avoid the spread of the cysts during the procedure. In this study, two immunoserologic tests for the diagnostic of hydatidosis are compared: Enzyme-linked Immunosorbent Assay (ELISA) for immunoglobulin G; and Fluoro-Enzyme Immunoassay (FEIA) for immunoglobulin E. Of the 55 patients included in this study are in a pre-surgery stage, 31% have a calcified hydatic cyst, 54,5% a not calcified cyst and 14.5% have no hydatic cyst. Both tests had the same specificity (87.5%), and the sensitivity of the ELISA IgG was lower (63.8%) than the FEIA IgE (76.6%). The correlation observed between both tests was good (r = 0.726, p < 0.05).
publishDate 2009
dc.date.none.fl_str_mv 2009-05-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.25758/set.204
https://doi.org/10.25758/set.204
url https://doi.org/10.25758/set.204
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://journals.ipl.pt/stecnologia/article/view/753
https://journals.ipl.pt/stecnologia/article/view/753/642
dc.rights.driver.fl_str_mv Direitos de Autor (c) 2023 Saúde & Tecnologia
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Direitos de Autor (c) 2023 Saúde & Tecnologia
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Escola Superior de Tecnologia da Saúde de Lisboa (Instituto Politécnico de Lisboa)
publisher.none.fl_str_mv Escola Superior de Tecnologia da Saúde de Lisboa (Instituto Politécnico de Lisboa)
dc.source.none.fl_str_mv Saúde e Tecnologia; No. 03 (2009): Maio 2009; 15-18
Saúde & Tecnologia; N.º 03 (2009): Maio 2009; 15-18
1646-9704
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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