Comparison of plantain plantlets propagated in temporary immersion bioreactors and gelled medium during in vitro growth and acclimatization
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/12389 |
Resumo: | The current work compared the physiological characteristics of plantain (Musa AAB) plantlets micropropagated in temporary immersion bioreactors (TIB) and on a gelled medium (GM). The plantlets were evaluated during in vitro growth (in the shoot elongation phase) and at the end of ex vitro acclimatization. TIB improved rooting and gave rise to longer shoots and higher dry mass. Respiration rate was the highest at the beginning of shoot elongation in both the TIB and GM plantlets. Photosynthetic rate in TIB was significantly higher than in GM from the midpoint of acclimatization, whereas a pyruvate kinase (PK) activity was lower. Starch accumulation was ca. two fold higher in corms than in leaves and always higher in the TIB than GM plantlets. The higher expression of genes coding for carbon metabolism enzymes PK and phosphoenolpyruvate carboxylase (PEPC) in TIB than in PM indicates a more important role of an autotrophic metabolism in the TIB plantlets when compared to the GM ones. The accumulated reserves were used during the first days of acclimatization leading to the higher survival rates and to the better plant quality of the TIB plantlets |
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Comparison of plantain plantlets propagated in temporary immersion bioreactors and gelled medium during in vitro growth and acclimatizationacclimatizationethyleneMusa spp.peroxiredoxinsphotosynthesisThe current work compared the physiological characteristics of plantain (Musa AAB) plantlets micropropagated in temporary immersion bioreactors (TIB) and on a gelled medium (GM). The plantlets were evaluated during in vitro growth (in the shoot elongation phase) and at the end of ex vitro acclimatization. TIB improved rooting and gave rise to longer shoots and higher dry mass. Respiration rate was the highest at the beginning of shoot elongation in both the TIB and GM plantlets. Photosynthetic rate in TIB was significantly higher than in GM from the midpoint of acclimatization, whereas a pyruvate kinase (PK) activity was lower. Starch accumulation was ca. two fold higher in corms than in leaves and always higher in the TIB than GM plantlets. The higher expression of genes coding for carbon metabolism enzymes PK and phosphoenolpyruvate carboxylase (PEPC) in TIB than in PM indicates a more important role of an autotrophic metabolism in the TIB plantlets when compared to the GM ones. The accumulated reserves were used during the first days of acclimatization leading to the higher survival rates and to the better plant quality of the TIB plantletss.n.Repositório da Universidade de LisboaAragón, C.E.Sanchez, C.Gonzalez-Olmedo, J.Escalona, M.Carvalho, L.Amâncio, S.2016-11-07T13:52:52Z20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/12389engAragón C, Sánchez C, González J, Escalona M, Carvalho L, Amâncio S (2014) Comparison of plantain plantlets propagated in temporary immersion bioreactors and gelled medium during in vitro growth and acclimatization. Biologia Plantarum 58: 29-38;10.1007/s10535-013-0381-6info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:42:35Zoai:www.repository.utl.pt:10400.5/12389Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:58:31.175777Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Comparison of plantain plantlets propagated in temporary immersion bioreactors and gelled medium during in vitro growth and acclimatization |
title |
Comparison of plantain plantlets propagated in temporary immersion bioreactors and gelled medium during in vitro growth and acclimatization |
spellingShingle |
Comparison of plantain plantlets propagated in temporary immersion bioreactors and gelled medium during in vitro growth and acclimatization Aragón, C.E. acclimatization ethylene Musa spp. peroxiredoxins photosynthesis |
title_short |
Comparison of plantain plantlets propagated in temporary immersion bioreactors and gelled medium during in vitro growth and acclimatization |
title_full |
Comparison of plantain plantlets propagated in temporary immersion bioreactors and gelled medium during in vitro growth and acclimatization |
title_fullStr |
Comparison of plantain plantlets propagated in temporary immersion bioreactors and gelled medium during in vitro growth and acclimatization |
title_full_unstemmed |
Comparison of plantain plantlets propagated in temporary immersion bioreactors and gelled medium during in vitro growth and acclimatization |
title_sort |
Comparison of plantain plantlets propagated in temporary immersion bioreactors and gelled medium during in vitro growth and acclimatization |
author |
Aragón, C.E. |
author_facet |
Aragón, C.E. Sanchez, C. Gonzalez-Olmedo, J. Escalona, M. Carvalho, L. Amâncio, S. |
author_role |
author |
author2 |
Sanchez, C. Gonzalez-Olmedo, J. Escalona, M. Carvalho, L. Amâncio, S. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Aragón, C.E. Sanchez, C. Gonzalez-Olmedo, J. Escalona, M. Carvalho, L. Amâncio, S. |
dc.subject.por.fl_str_mv |
acclimatization ethylene Musa spp. peroxiredoxins photosynthesis |
topic |
acclimatization ethylene Musa spp. peroxiredoxins photosynthesis |
description |
The current work compared the physiological characteristics of plantain (Musa AAB) plantlets micropropagated in temporary immersion bioreactors (TIB) and on a gelled medium (GM). The plantlets were evaluated during in vitro growth (in the shoot elongation phase) and at the end of ex vitro acclimatization. TIB improved rooting and gave rise to longer shoots and higher dry mass. Respiration rate was the highest at the beginning of shoot elongation in both the TIB and GM plantlets. Photosynthetic rate in TIB was significantly higher than in GM from the midpoint of acclimatization, whereas a pyruvate kinase (PK) activity was lower. Starch accumulation was ca. two fold higher in corms than in leaves and always higher in the TIB than GM plantlets. The higher expression of genes coding for carbon metabolism enzymes PK and phosphoenolpyruvate carboxylase (PEPC) in TIB than in PM indicates a more important role of an autotrophic metabolism in the TIB plantlets when compared to the GM ones. The accumulated reserves were used during the first days of acclimatization leading to the higher survival rates and to the better plant quality of the TIB plantlets |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z 2016-11-07T13:52:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/12389 |
url |
http://hdl.handle.net/10400.5/12389 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Aragón C, Sánchez C, González J, Escalona M, Carvalho L, Amâncio S (2014) Comparison of plantain plantlets propagated in temporary immersion bioreactors and gelled medium during in vitro growth and acclimatization. Biologia Plantarum 58: 29-38; 10.1007/s10535-013-0381-6 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
s.n. |
publisher.none.fl_str_mv |
s.n. |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131071659376640 |