TCTEX1D4 interactome in human testis: unraveling the function of dynein light chain in spermatozoa
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/29396 |
Resumo: | Studies were designed to identify the TCTEX1D4 interactome in human testis, with the purpose of unraveling putative protein complexes essential to male reproduction and thus novel TCTEX1D4 functions. TCTEX1D4 is a dynein light chain that belongs to the DYNT1/TCTEX1 family. In spermatozoa, it appears to be important to sperm motility, intraflagellar transport, and acrosome reaction. To contribute to the knowledge on TCTEX1D4 function in testis and spermatozoa, a yeast two-hybrid assay was performed in testis, which allowed the identification of 40 novel TCTEX1D4 interactors. Curiously, another dynein light chain, TCTEX1D2, was identified and its existence demonstrated for the first time in human spermatozoa. Immunofluorescence studies proved that TCTEX1D2 is an intra-acrosomal protein also present in the midpiece, suggesting a role in cargo movement in human spermatozoa. Further, an in silico profile of TCTEX1D4 revealed that most TCTEX1D4 interacting proteins were not previously characterized and the ones described present a very broad nature. This reinforces TCTEX1D4 as a dynein light chain that is capable of interacting with a variety of functionally different proteins. These observations collectively contribute to a deeper molecular understanding of the human spermatozoa function. |
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TCTEX1D4 interactome in human testis: unraveling the function of dynein light chain in spermatozoaTCTEX1D4TCTEX1D2PPP1CC2InteractomeIn silicoAcrosome reactionMotilityStudies were designed to identify the TCTEX1D4 interactome in human testis, with the purpose of unraveling putative protein complexes essential to male reproduction and thus novel TCTEX1D4 functions. TCTEX1D4 is a dynein light chain that belongs to the DYNT1/TCTEX1 family. In spermatozoa, it appears to be important to sperm motility, intraflagellar transport, and acrosome reaction. To contribute to the knowledge on TCTEX1D4 function in testis and spermatozoa, a yeast two-hybrid assay was performed in testis, which allowed the identification of 40 novel TCTEX1D4 interactors. Curiously, another dynein light chain, TCTEX1D2, was identified and its existence demonstrated for the first time in human spermatozoa. Immunofluorescence studies proved that TCTEX1D2 is an intra-acrosomal protein also present in the midpiece, suggesting a role in cargo movement in human spermatozoa. Further, an in silico profile of TCTEX1D4 revealed that most TCTEX1D4 interacting proteins were not previously characterized and the ones described present a very broad nature. This reinforces TCTEX1D4 as a dynein light chain that is capable of interacting with a variety of functionally different proteins. These observations collectively contribute to a deeper molecular understanding of the human spermatozoa function.Mary Ann Liebert2020-10-09T16:48:01Z2014-04-01T00:00:00Z2014-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/29396eng1536-231010.1089/omi.2013.0133Freitas, Maria JoãoKorrodi-Gregório, LuísMorais-Santos, FilipaSilva, Edgar da Cruz eFardilha, Margaridainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:56:34Zoai:ria.ua.pt:10773/29396Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:01:37.759349Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
TCTEX1D4 interactome in human testis: unraveling the function of dynein light chain in spermatozoa |
title |
TCTEX1D4 interactome in human testis: unraveling the function of dynein light chain in spermatozoa |
spellingShingle |
TCTEX1D4 interactome in human testis: unraveling the function of dynein light chain in spermatozoa Freitas, Maria João TCTEX1D4 TCTEX1D2 PPP1CC2 Interactome In silico Acrosome reaction Motility |
title_short |
TCTEX1D4 interactome in human testis: unraveling the function of dynein light chain in spermatozoa |
title_full |
TCTEX1D4 interactome in human testis: unraveling the function of dynein light chain in spermatozoa |
title_fullStr |
TCTEX1D4 interactome in human testis: unraveling the function of dynein light chain in spermatozoa |
title_full_unstemmed |
TCTEX1D4 interactome in human testis: unraveling the function of dynein light chain in spermatozoa |
title_sort |
TCTEX1D4 interactome in human testis: unraveling the function of dynein light chain in spermatozoa |
author |
Freitas, Maria João |
author_facet |
Freitas, Maria João Korrodi-Gregório, Luís Morais-Santos, Filipa Silva, Edgar da Cruz e Fardilha, Margarida |
author_role |
author |
author2 |
Korrodi-Gregório, Luís Morais-Santos, Filipa Silva, Edgar da Cruz e Fardilha, Margarida |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Freitas, Maria João Korrodi-Gregório, Luís Morais-Santos, Filipa Silva, Edgar da Cruz e Fardilha, Margarida |
dc.subject.por.fl_str_mv |
TCTEX1D4 TCTEX1D2 PPP1CC2 Interactome In silico Acrosome reaction Motility |
topic |
TCTEX1D4 TCTEX1D2 PPP1CC2 Interactome In silico Acrosome reaction Motility |
description |
Studies were designed to identify the TCTEX1D4 interactome in human testis, with the purpose of unraveling putative protein complexes essential to male reproduction and thus novel TCTEX1D4 functions. TCTEX1D4 is a dynein light chain that belongs to the DYNT1/TCTEX1 family. In spermatozoa, it appears to be important to sperm motility, intraflagellar transport, and acrosome reaction. To contribute to the knowledge on TCTEX1D4 function in testis and spermatozoa, a yeast two-hybrid assay was performed in testis, which allowed the identification of 40 novel TCTEX1D4 interactors. Curiously, another dynein light chain, TCTEX1D2, was identified and its existence demonstrated for the first time in human spermatozoa. Immunofluorescence studies proved that TCTEX1D2 is an intra-acrosomal protein also present in the midpiece, suggesting a role in cargo movement in human spermatozoa. Further, an in silico profile of TCTEX1D4 revealed that most TCTEX1D4 interacting proteins were not previously characterized and the ones described present a very broad nature. This reinforces TCTEX1D4 as a dynein light chain that is capable of interacting with a variety of functionally different proteins. These observations collectively contribute to a deeper molecular understanding of the human spermatozoa function. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-04-01T00:00:00Z 2014-04 2020-10-09T16:48:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/29396 |
url |
http://hdl.handle.net/10773/29396 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1536-2310 10.1089/omi.2013.0133 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Mary Ann Liebert |
publisher.none.fl_str_mv |
Mary Ann Liebert |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137671848656896 |