Chemical characterization, bioactivity and toxicity of european flora plant extracts in search for potential natural origin preservatives
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/28263 |
Resumo: | Consumer demand for natural and healthier products has led to an increasing interest in the bioactive and therapeutic properties of plant extracts. In this study, we evaluated the phenolic compounds profile, bioactivities, and toxicities of plant extracts from eight European flora species, including Calendula officinalis L., Calluna vulgaris (L.) Hull, Hippophae rhamnoides L., Juglans regia L., Mentha cervina L., Rubus idaeus L., Sambucus nigra L., and Vitis vinifera L. The aim was to identify potential preservatives of natural origin. Phenolic compounds were identified by HPLC-DAD-ESI-MS. Caffeic acid derivatives, ellagitannins, flavonols, and flavones were the major phenolic compounds identified. The total phenolic content varied from 16.0 & PLUSMN; 0.2 (V. vinifera) to 123 & PLUSMN; 2 mg/g (H. rhamnoides) of dry extract. All extracts showed antioxidant potential and exhibited activity against some of the microorganisms tested. S. nigra showed the highest activity in the inhibition of oxidative hemolysis (OxHLIA) assay and H. rhamnoides, notably, had the lowest IC50 values in TBARS and DPPH assays, as well as the lowest minimum inhibitory concentration (MIC) values. Regarding in vitro cytotoxicity, in tumor and non-tumor cell lines, although some extracts revealed toxicity against normal cells, it was found that the samples C. vulgaris, V. vinifera and R. idaeus might be used against tumor cells since the active concentration is much lower than the one causing toxicity. In vivo acute toxicity tests using Artemia franciscana suggest low toxicity for most extracts, with LC50 > 400 mg/L. These results showed the potential of the studied extracts as natural preservatives, given their richness in compounds with bioactive properties, highlight their potential value to the production chain. |
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Chemical characterization, bioactivity and toxicity of european flora plant extracts in search for potential natural origin preservativesPhenolic compoundsPlant extractsNatural preservativesBioactivityToxicityArtemia franciscanaEuropean floraConsumer demand for natural and healthier products has led to an increasing interest in the bioactive and therapeutic properties of plant extracts. In this study, we evaluated the phenolic compounds profile, bioactivities, and toxicities of plant extracts from eight European flora species, including Calendula officinalis L., Calluna vulgaris (L.) Hull, Hippophae rhamnoides L., Juglans regia L., Mentha cervina L., Rubus idaeus L., Sambucus nigra L., and Vitis vinifera L. The aim was to identify potential preservatives of natural origin. Phenolic compounds were identified by HPLC-DAD-ESI-MS. Caffeic acid derivatives, ellagitannins, flavonols, and flavones were the major phenolic compounds identified. The total phenolic content varied from 16.0 & PLUSMN; 0.2 (V. vinifera) to 123 & PLUSMN; 2 mg/g (H. rhamnoides) of dry extract. All extracts showed antioxidant potential and exhibited activity against some of the microorganisms tested. S. nigra showed the highest activity in the inhibition of oxidative hemolysis (OxHLIA) assay and H. rhamnoides, notably, had the lowest IC50 values in TBARS and DPPH assays, as well as the lowest minimum inhibitory concentration (MIC) values. Regarding in vitro cytotoxicity, in tumor and non-tumor cell lines, although some extracts revealed toxicity against normal cells, it was found that the samples C. vulgaris, V. vinifera and R. idaeus might be used against tumor cells since the active concentration is much lower than the one causing toxicity. In vivo acute toxicity tests using Artemia franciscana suggest low toxicity for most extracts, with LC50 > 400 mg/L. These results showed the potential of the studied extracts as natural preservatives, given their richness in compounds with bioactive properties, highlight their potential value to the production chain.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2020). National funding was given by FCT, P.I., through the institutional scientific employment programcontracts of L. Barros and S.A. Heleno, and the PhD grant of F. Mandim (SFRH/BD/146614/2019), and we also thank the MICINN for the Juan de la Cierva Formación contract for T.C.S.P. Pires (FJC20120-045405-I). This manuscript has received funding from the Bio Based Industries Joint Undertaking (JU) under grant agreement No 887917 BeonNAT.MDPIBiblioteca Digital do IPBDeus, Breno Martins deFernandes, ConceiçãoMolina, Adriana K.Xavier, VirginiePires, Tânia C.S.P.Mandim, FilipaHeleno, Sandrina A.Finimundy, Tiane C.Barros, Lillian2023-05-10T09:16:39Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/28263engDeus, Breno Martins de; Fernandes, Conceição; Molina, Adriana K.; Xavier, Virginie; Pires, Tânia C.S.P.; Mandim, Filipa; Heleno, Sandrina A.; Finimundy, Tiane C.; Barros, Lillian (2023). Chemical characterization, bioactivity and toxicity of european flora plant extracts in search for potential natural origin preservatives. Plants. eISSN 2223-7747. 12:15, p. 1-1810.3390/plants121527842223-7747info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-06T01:17:43Zoai:bibliotecadigital.ipb.pt:10198/28263Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:41:19.499741Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Chemical characterization, bioactivity and toxicity of european flora plant extracts in search for potential natural origin preservatives |
title |
Chemical characterization, bioactivity and toxicity of european flora plant extracts in search for potential natural origin preservatives |
spellingShingle |
Chemical characterization, bioactivity and toxicity of european flora plant extracts in search for potential natural origin preservatives Deus, Breno Martins de Phenolic compounds Plant extracts Natural preservatives Bioactivity Toxicity Artemia franciscana European flora |
title_short |
Chemical characterization, bioactivity and toxicity of european flora plant extracts in search for potential natural origin preservatives |
title_full |
Chemical characterization, bioactivity and toxicity of european flora plant extracts in search for potential natural origin preservatives |
title_fullStr |
Chemical characterization, bioactivity and toxicity of european flora plant extracts in search for potential natural origin preservatives |
title_full_unstemmed |
Chemical characterization, bioactivity and toxicity of european flora plant extracts in search for potential natural origin preservatives |
title_sort |
Chemical characterization, bioactivity and toxicity of european flora plant extracts in search for potential natural origin preservatives |
author |
Deus, Breno Martins de |
author_facet |
Deus, Breno Martins de Fernandes, Conceição Molina, Adriana K. Xavier, Virginie Pires, Tânia C.S.P. Mandim, Filipa Heleno, Sandrina A. Finimundy, Tiane C. Barros, Lillian |
author_role |
author |
author2 |
Fernandes, Conceição Molina, Adriana K. Xavier, Virginie Pires, Tânia C.S.P. Mandim, Filipa Heleno, Sandrina A. Finimundy, Tiane C. Barros, Lillian |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Deus, Breno Martins de Fernandes, Conceição Molina, Adriana K. Xavier, Virginie Pires, Tânia C.S.P. Mandim, Filipa Heleno, Sandrina A. Finimundy, Tiane C. Barros, Lillian |
dc.subject.por.fl_str_mv |
Phenolic compounds Plant extracts Natural preservatives Bioactivity Toxicity Artemia franciscana European flora |
topic |
Phenolic compounds Plant extracts Natural preservatives Bioactivity Toxicity Artemia franciscana European flora |
description |
Consumer demand for natural and healthier products has led to an increasing interest in the bioactive and therapeutic properties of plant extracts. In this study, we evaluated the phenolic compounds profile, bioactivities, and toxicities of plant extracts from eight European flora species, including Calendula officinalis L., Calluna vulgaris (L.) Hull, Hippophae rhamnoides L., Juglans regia L., Mentha cervina L., Rubus idaeus L., Sambucus nigra L., and Vitis vinifera L. The aim was to identify potential preservatives of natural origin. Phenolic compounds were identified by HPLC-DAD-ESI-MS. Caffeic acid derivatives, ellagitannins, flavonols, and flavones were the major phenolic compounds identified. The total phenolic content varied from 16.0 & PLUSMN; 0.2 (V. vinifera) to 123 & PLUSMN; 2 mg/g (H. rhamnoides) of dry extract. All extracts showed antioxidant potential and exhibited activity against some of the microorganisms tested. S. nigra showed the highest activity in the inhibition of oxidative hemolysis (OxHLIA) assay and H. rhamnoides, notably, had the lowest IC50 values in TBARS and DPPH assays, as well as the lowest minimum inhibitory concentration (MIC) values. Regarding in vitro cytotoxicity, in tumor and non-tumor cell lines, although some extracts revealed toxicity against normal cells, it was found that the samples C. vulgaris, V. vinifera and R. idaeus might be used against tumor cells since the active concentration is much lower than the one causing toxicity. In vivo acute toxicity tests using Artemia franciscana suggest low toxicity for most extracts, with LC50 > 400 mg/L. These results showed the potential of the studied extracts as natural preservatives, given their richness in compounds with bioactive properties, highlight their potential value to the production chain. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-05-10T09:16:39Z 2023 2023-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/28263 |
url |
http://hdl.handle.net/10198/28263 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Deus, Breno Martins de; Fernandes, Conceição; Molina, Adriana K.; Xavier, Virginie; Pires, Tânia C.S.P.; Mandim, Filipa; Heleno, Sandrina A.; Finimundy, Tiane C.; Barros, Lillian (2023). Chemical characterization, bioactivity and toxicity of european flora plant extracts in search for potential natural origin preservatives. Plants. eISSN 2223-7747. 12:15, p. 1-18 10.3390/plants12152784 2223-7747 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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MDPI |
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MDPI |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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