Epidermal growth factor alters silica nanoparticle uptake and improves gold-nanoparticle-mediated gene silencing in A549 cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/164441 |
Resumo: | Funding Information: The authors would like to acknowledge the financial support of the NCCR Bioinspired Materials through the Swiss National Science Foundation, the Adolphe Merkle Foundation. Thank you for the support from FCT—Fundação para a Ciência e a Tecnologia, I.P., in the scope of the project 2022.04315.PTDC, and UIDP/04378/2020 and UIDB/04378/2020—UCIBIO and LA/P/0140/2020—i4HB, 2020.07660.BD and 2020.06599.BD. Aura Maria Moreno-Echeverri and Barbara Rothen-Rutishauser acknowledge the Swiss National Science Foundation (SNSF) (project 310030_192056/1). Funding Information: The authors would like to acknowledge the financial support of the NCCR Bioinspired Materials through the Swiss National Science Foundation, the Adolphe Merkle Foundation. Thank you for the support from FCT—Fundação para a Ciência e a Tecnologia, I.P., in the scope of the project 2022.04315.PTDC, and UIDP/04378/2020 and UIDB/04378/2020—UCIBIO and LA/P/0140/2020—i4HB, 2020.07660.BD and 2020.06599.BD. Aura Maria Moreno-Echeverri and Barbara Rothen-Rutishauser acknowledge the Swiss National Science Foundation (SNSF) (project 310030_192056/1). Publisher Copyright: Copyright © 2023 Susnik, Bazzoni, Taladriz-Blanco, Balog, Moreno-Echeverri, Glaubitz, Brito Oliveira, Ferreira, Viana Baptista, Petri-Fink and Rothen-Rutishauser. |
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Epidermal growth factor alters silica nanoparticle uptake and improves gold-nanoparticle-mediated gene silencing in A549 cellsendocytosisepidermal growth factorgene silencinggoldmolecular mechanismsnanomedicinenanoparticlessilicaElectronic, Optical and Magnetic MaterialsAtomic and Molecular Physics, and OpticsBiomedical EngineeringComputer Science ApplicationsElectrical and Electronic EngineeringSDG 3 - Good Health and Well-beingFunding Information: The authors would like to acknowledge the financial support of the NCCR Bioinspired Materials through the Swiss National Science Foundation, the Adolphe Merkle Foundation. Thank you for the support from FCT—Fundação para a Ciência e a Tecnologia, I.P., in the scope of the project 2022.04315.PTDC, and UIDP/04378/2020 and UIDB/04378/2020—UCIBIO and LA/P/0140/2020—i4HB, 2020.07660.BD and 2020.06599.BD. Aura Maria Moreno-Echeverri and Barbara Rothen-Rutishauser acknowledge the Swiss National Science Foundation (SNSF) (project 310030_192056/1). Funding Information: The authors would like to acknowledge the financial support of the NCCR Bioinspired Materials through the Swiss National Science Foundation, the Adolphe Merkle Foundation. Thank you for the support from FCT—Fundação para a Ciência e a Tecnologia, I.P., in the scope of the project 2022.04315.PTDC, and UIDP/04378/2020 and UIDB/04378/2020—UCIBIO and LA/P/0140/2020—i4HB, 2020.07660.BD and 2020.06599.BD. Aura Maria Moreno-Echeverri and Barbara Rothen-Rutishauser acknowledge the Swiss National Science Foundation (SNSF) (project 310030_192056/1). Publisher Copyright: Copyright © 2023 Susnik, Bazzoni, Taladriz-Blanco, Balog, Moreno-Echeverri, Glaubitz, Brito Oliveira, Ferreira, Viana Baptista, Petri-Fink and Rothen-Rutishauser.Introduction: Delivery of therapeutic nanoparticles (NPs) to cancer cells represents a promising approach for biomedical applications. A key challenge for nanotechnology translation from the bench to the bedside is the low amount of administered NPs dose that effectively enters target cells. To improve NPs delivery, several studies proposed NPs conjugation with ligands, which specifically deliver NPs to target cells via receptor binding. One such example is epidermal growth factor (EGF), a peptide involved in cell signaling pathways that control cell division by binding to epidermal growth factor receptor (EGFR). However, very few studies assessed the influence of EGF present in the cell environment, on the cellular uptake of NPs. Methods: We tested if the stimulation of EGFR-expressing lung carcinomacells A549 with EGF affects the uptake of 59 nm and 422 nm silica (SiO2) NPs. Additionally, we investigated whether the uptake enhancement can be achieved with gold NPs, suitable to downregulate the expression of cancer oncogene c-MYC. Results: Our findings show that EGF binding to its receptor results in receptor autophosphorylation and initiate signaling pathways, leading to enhanced endocytosis of 59 nm SiO2 NPs, but not 422 nm SiO2 NPs. Additionally, we demonstrated an enhanced gold (Au) NPs endocytosis and subsequently a higher downregulation of c-MYC. Discussion: These findings contribute to a better understanding of NPs uptake in the presence of EGF and that is a promising approach for improved NPs delivery.DCV - Departamento de Ciências da VidaUCIBIO - Applied Molecular Biosciences UnitRUNSusnik, EvaBazzoni, AmelieTaladriz-Blanco, PatriciaBalog, SandorMoreno-Echeverri, Aura MariaGlaubitz, ChristinaBrito Oliveira, BeatrizFerreira, DanielaViana Baptista, PedroPetri-Fink, AlkeRothen-Rutishauser, Barbara2024-03-05T00:18:39Z2023-07-172023-07-17T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17application/pdfhttp://hdl.handle.net/10362/164441eng2673-3013PURE: 84484559https://doi.org/10.3389/fnano.2023.1220514info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:52:18Zoai:run.unl.pt:10362/164441Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T04:00:10.775016Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Epidermal growth factor alters silica nanoparticle uptake and improves gold-nanoparticle-mediated gene silencing in A549 cells |
| title |
Epidermal growth factor alters silica nanoparticle uptake and improves gold-nanoparticle-mediated gene silencing in A549 cells |
| spellingShingle |
Epidermal growth factor alters silica nanoparticle uptake and improves gold-nanoparticle-mediated gene silencing in A549 cells Susnik, Eva endocytosis epidermal growth factor gene silencing gold molecular mechanisms nanomedicine nanoparticles silica Electronic, Optical and Magnetic Materials Atomic and Molecular Physics, and Optics Biomedical Engineering Computer Science Applications Electrical and Electronic Engineering SDG 3 - Good Health and Well-being |
| title_short |
Epidermal growth factor alters silica nanoparticle uptake and improves gold-nanoparticle-mediated gene silencing in A549 cells |
| title_full |
Epidermal growth factor alters silica nanoparticle uptake and improves gold-nanoparticle-mediated gene silencing in A549 cells |
| title_fullStr |
Epidermal growth factor alters silica nanoparticle uptake and improves gold-nanoparticle-mediated gene silencing in A549 cells |
| title_full_unstemmed |
Epidermal growth factor alters silica nanoparticle uptake and improves gold-nanoparticle-mediated gene silencing in A549 cells |
| title_sort |
Epidermal growth factor alters silica nanoparticle uptake and improves gold-nanoparticle-mediated gene silencing in A549 cells |
| author |
Susnik, Eva |
| author_facet |
Susnik, Eva Bazzoni, Amelie Taladriz-Blanco, Patricia Balog, Sandor Moreno-Echeverri, Aura Maria Glaubitz, Christina Brito Oliveira, Beatriz Ferreira, Daniela Viana Baptista, Pedro Petri-Fink, Alke Rothen-Rutishauser, Barbara |
| author_role |
author |
| author2 |
Bazzoni, Amelie Taladriz-Blanco, Patricia Balog, Sandor Moreno-Echeverri, Aura Maria Glaubitz, Christina Brito Oliveira, Beatriz Ferreira, Daniela Viana Baptista, Pedro Petri-Fink, Alke Rothen-Rutishauser, Barbara |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
DCV - Departamento de Ciências da Vida UCIBIO - Applied Molecular Biosciences Unit RUN |
| dc.contributor.author.fl_str_mv |
Susnik, Eva Bazzoni, Amelie Taladriz-Blanco, Patricia Balog, Sandor Moreno-Echeverri, Aura Maria Glaubitz, Christina Brito Oliveira, Beatriz Ferreira, Daniela Viana Baptista, Pedro Petri-Fink, Alke Rothen-Rutishauser, Barbara |
| dc.subject.por.fl_str_mv |
endocytosis epidermal growth factor gene silencing gold molecular mechanisms nanomedicine nanoparticles silica Electronic, Optical and Magnetic Materials Atomic and Molecular Physics, and Optics Biomedical Engineering Computer Science Applications Electrical and Electronic Engineering SDG 3 - Good Health and Well-being |
| topic |
endocytosis epidermal growth factor gene silencing gold molecular mechanisms nanomedicine nanoparticles silica Electronic, Optical and Magnetic Materials Atomic and Molecular Physics, and Optics Biomedical Engineering Computer Science Applications Electrical and Electronic Engineering SDG 3 - Good Health and Well-being |
| description |
Funding Information: The authors would like to acknowledge the financial support of the NCCR Bioinspired Materials through the Swiss National Science Foundation, the Adolphe Merkle Foundation. Thank you for the support from FCT—Fundação para a Ciência e a Tecnologia, I.P., in the scope of the project 2022.04315.PTDC, and UIDP/04378/2020 and UIDB/04378/2020—UCIBIO and LA/P/0140/2020—i4HB, 2020.07660.BD and 2020.06599.BD. Aura Maria Moreno-Echeverri and Barbara Rothen-Rutishauser acknowledge the Swiss National Science Foundation (SNSF) (project 310030_192056/1). Funding Information: The authors would like to acknowledge the financial support of the NCCR Bioinspired Materials through the Swiss National Science Foundation, the Adolphe Merkle Foundation. Thank you for the support from FCT—Fundação para a Ciência e a Tecnologia, I.P., in the scope of the project 2022.04315.PTDC, and UIDP/04378/2020 and UIDB/04378/2020—UCIBIO and LA/P/0140/2020—i4HB, 2020.07660.BD and 2020.06599.BD. Aura Maria Moreno-Echeverri and Barbara Rothen-Rutishauser acknowledge the Swiss National Science Foundation (SNSF) (project 310030_192056/1). Publisher Copyright: Copyright © 2023 Susnik, Bazzoni, Taladriz-Blanco, Balog, Moreno-Echeverri, Glaubitz, Brito Oliveira, Ferreira, Viana Baptista, Petri-Fink and Rothen-Rutishauser. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-07-17 2023-07-17T00:00:00Z 2024-03-05T00:18:39Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10362/164441 |
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http://hdl.handle.net/10362/164441 |
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eng |
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eng |
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2673-3013 PURE: 84484559 https://doi.org/10.3389/fnano.2023.1220514 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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17 application/pdf |
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