Thermal effect on aequorea green fluorescent protein anionic and neutral chromophore forms fluorescence

Detalhes bibliográficos
Autor(a) principal: Santos, Andrea Martins dos
Data de Publicação: 2012
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/10789
Resumo: © Springer Science+Business Media, LLC 2011.
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spelling Thermal effect on aequorea green fluorescent protein anionic and neutral chromophore forms fluorescenceGreen fluorescent proteinChromophoreFluorescenceTemperature© Springer Science+Business Media, LLC 2011.The emission behaviour of Aequorea green fluorescent protein (A-GFP) chromophore, in both neutral (N) and anionic (A) form, was studied in the temperature range from 20 °C to 75 °C and at pH=7. Excitation wavelengths of 399 nm and 476 nm were applied to probe the N and A forms environment, respectively. Both forms exhibit distinct fluorescence patterns at high temperature values. The emission quenching rate, following a temperature increase, is higher for the chromophore N form as a result of the hydrogen bond network weakening. The chromophore anionic form emission maximum is red shifted, upon temperature increase, due to a charge transfer process occurring after A form excitation.The author acknowledges financial support from Fundação para a Ciência e a Tecnologia through grant SFRH/BPD/26821/2006. Dr. V. Subramanian (University of Twente,NL) for providing the A-GFP gene, and Dr. F. Mergulhão (University of Porto, PT) for the support given on A-GFP expression and purification.SpringerRepositório da Universidade de LisboaSantos, Andrea Martins dos2014-03-27T14:45:41Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/10789engJ Fluoresc (2012) 22:151–1541053-0509http://dx.doi.org/10.1007/s10895-011-0941-0metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:56:32Zoai:repositorio.ul.pt:10451/10789Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:34:41.996326Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Thermal effect on aequorea green fluorescent protein anionic and neutral chromophore forms fluorescence
title Thermal effect on aequorea green fluorescent protein anionic and neutral chromophore forms fluorescence
spellingShingle Thermal effect on aequorea green fluorescent protein anionic and neutral chromophore forms fluorescence
Santos, Andrea Martins dos
Green fluorescent protein
Chromophore
Fluorescence
Temperature
title_short Thermal effect on aequorea green fluorescent protein anionic and neutral chromophore forms fluorescence
title_full Thermal effect on aequorea green fluorescent protein anionic and neutral chromophore forms fluorescence
title_fullStr Thermal effect on aequorea green fluorescent protein anionic and neutral chromophore forms fluorescence
title_full_unstemmed Thermal effect on aequorea green fluorescent protein anionic and neutral chromophore forms fluorescence
title_sort Thermal effect on aequorea green fluorescent protein anionic and neutral chromophore forms fluorescence
author Santos, Andrea Martins dos
author_facet Santos, Andrea Martins dos
author_role author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Santos, Andrea Martins dos
dc.subject.por.fl_str_mv Green fluorescent protein
Chromophore
Fluorescence
Temperature
topic Green fluorescent protein
Chromophore
Fluorescence
Temperature
description © Springer Science+Business Media, LLC 2011.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
2014-03-27T14:45:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/10789
url http://hdl.handle.net/10451/10789
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv J Fluoresc (2012) 22:151–154
1053-0509
http://dx.doi.org/10.1007/s10895-011-0941-0
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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