Assessing the Influence of Betaine-Based Natural Deep Eutectic Systems on Horseradish Peroxidase

Detalhes bibliográficos
Autor(a) principal: Gajardo-Parra, Nicolás F.
Data de Publicação: 2022
Outros Autores: Meneses, Liane, Duarte, Ana Rita C., Paiva, Alexandre, Held, Christoph
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/160713
Resumo: Funding Information: The work of Nicolás Gajardo was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy - EXC 2033 - 390677874–RESOLV and by the German Academic Exchange Service (DAAD) under the Graduate School Scholarship Programme, 2020 (57516591). This work has also received funding from the European Union’s Horizon 2020 (European Research Council) under grant agreement no ERC-2016-CoG 725034. This work was further supported by the Associate Laboratory for Green Chemistry - LAQV and the project CryoDES which are financed by national funds from FCT/MCTES: UIDB/50006/2020 and PTDC/EQU–EQU/29851/2017, respectively. A.P. and L.M. also acknowledge FCT/MCTES for the financial support through IF/01146/2015 and SFRH/BD/148510/2019, respectively. The authors wish to thank Elisabete Ferreira from the BioLab, supported by the Applied Molecular Biosciences Research Unit-UCIBIO and the Associated Laboratory for Green Chemistry Research Unit–LAQV (UIDP/04378/2020, UIDB/04378/2020 and UIDB/50006/2020, UIDP/50006/2020, respectively). Publisher Copyright: © 2022 American Chemical Society. All rights reserved.
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spelling Assessing the Influence of Betaine-Based Natural Deep Eutectic Systems on Horseradish Peroxidasebiocatalysisdensitynatural deep eutectic systems (NADES)perturbed-chain statistical associating fluid theory (PC-SAFT)thermodynamicsviscositywater activityChemistry(all)Environmental ChemistryChemical Engineering(all)Renewable Energy, Sustainability and the EnvironmentSDG 7 - Affordable and Clean EnergyFunding Information: The work of Nicolás Gajardo was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy - EXC 2033 - 390677874–RESOLV and by the German Academic Exchange Service (DAAD) under the Graduate School Scholarship Programme, 2020 (57516591). This work has also received funding from the European Union’s Horizon 2020 (European Research Council) under grant agreement no ERC-2016-CoG 725034. This work was further supported by the Associate Laboratory for Green Chemistry - LAQV and the project CryoDES which are financed by national funds from FCT/MCTES: UIDB/50006/2020 and PTDC/EQU–EQU/29851/2017, respectively. A.P. and L.M. also acknowledge FCT/MCTES for the financial support through IF/01146/2015 and SFRH/BD/148510/2019, respectively. The authors wish to thank Elisabete Ferreira from the BioLab, supported by the Applied Molecular Biosciences Research Unit-UCIBIO and the Associated Laboratory for Green Chemistry Research Unit–LAQV (UIDP/04378/2020, UIDB/04378/2020 and UIDB/50006/2020, UIDP/50006/2020, respectively). Publisher Copyright: © 2022 American Chemical Society. All rights reserved.To validate the use of horseradish peroxidase (HRP) in natural deep eutectic systems (NADES), five different betaine-based NADES were characterized in terms of water content, water activity, density, and viscosity experimentally and by thermodynamic modeling. The results show that the NADES under study have a water activity of about 0.4 at 37 °C for water contents between 14 and 22 wt %. The densities of the studied NADES had values between 1.2 and 1.3 g.cm-3at 20 °C. The density was modeled with a state-of-the-art equation of state; an excellent agreement with the experimental density data was achieved, allowing reasonable predictions for water activities. The system betaine:glycerol (1:2) was found to be the most viscous with a dynamic viscosity of ∼600 mPa.s at 40 °C, while all the other systems had viscosities <350 mPa.s at 40 °C. The impact of the NADES on the enzymatic activity, as well as on, conformational and thermal stability was assessed. The system betaine/sorbitol:water (1:1:3) showed the highest benefit for enzymatic activity, increasing it by two-folds. Moreover, upon NADES addition, thermal stability was increased followed by an increment in a-helix secondary structure content.LAQV@REQUIMTEDQ - Departamento de QuímicaRUNGajardo-Parra, Nicolás F.Meneses, LianeDuarte, Ana Rita C.Paiva, AlexandreHeld, Christoph2023-11-29T22:29:41Z2022-09-262022-09-26T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttp://hdl.handle.net/10362/160713eng2168-0485PURE: 71539876https://doi.org/10.1021/acssuschemeng.2c04045info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:43:28Zoai:run.unl.pt:10362/160713Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:58:11.464766Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Assessing the Influence of Betaine-Based Natural Deep Eutectic Systems on Horseradish Peroxidase
title Assessing the Influence of Betaine-Based Natural Deep Eutectic Systems on Horseradish Peroxidase
spellingShingle Assessing the Influence of Betaine-Based Natural Deep Eutectic Systems on Horseradish Peroxidase
Gajardo-Parra, Nicolás F.
biocatalysis
density
natural deep eutectic systems (NADES)
perturbed-chain statistical associating fluid theory (PC-SAFT)
thermodynamics
viscosity
water activity
Chemistry(all)
Environmental Chemistry
Chemical Engineering(all)
Renewable Energy, Sustainability and the Environment
SDG 7 - Affordable and Clean Energy
title_short Assessing the Influence of Betaine-Based Natural Deep Eutectic Systems on Horseradish Peroxidase
title_full Assessing the Influence of Betaine-Based Natural Deep Eutectic Systems on Horseradish Peroxidase
title_fullStr Assessing the Influence of Betaine-Based Natural Deep Eutectic Systems on Horseradish Peroxidase
title_full_unstemmed Assessing the Influence of Betaine-Based Natural Deep Eutectic Systems on Horseradish Peroxidase
title_sort Assessing the Influence of Betaine-Based Natural Deep Eutectic Systems on Horseradish Peroxidase
author Gajardo-Parra, Nicolás F.
author_facet Gajardo-Parra, Nicolás F.
Meneses, Liane
Duarte, Ana Rita C.
Paiva, Alexandre
Held, Christoph
author_role author
author2 Meneses, Liane
Duarte, Ana Rita C.
Paiva, Alexandre
Held, Christoph
author2_role author
author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Gajardo-Parra, Nicolás F.
Meneses, Liane
Duarte, Ana Rita C.
Paiva, Alexandre
Held, Christoph
dc.subject.por.fl_str_mv biocatalysis
density
natural deep eutectic systems (NADES)
perturbed-chain statistical associating fluid theory (PC-SAFT)
thermodynamics
viscosity
water activity
Chemistry(all)
Environmental Chemistry
Chemical Engineering(all)
Renewable Energy, Sustainability and the Environment
SDG 7 - Affordable and Clean Energy
topic biocatalysis
density
natural deep eutectic systems (NADES)
perturbed-chain statistical associating fluid theory (PC-SAFT)
thermodynamics
viscosity
water activity
Chemistry(all)
Environmental Chemistry
Chemical Engineering(all)
Renewable Energy, Sustainability and the Environment
SDG 7 - Affordable and Clean Energy
description Funding Information: The work of Nicolás Gajardo was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy - EXC 2033 - 390677874–RESOLV and by the German Academic Exchange Service (DAAD) under the Graduate School Scholarship Programme, 2020 (57516591). This work has also received funding from the European Union’s Horizon 2020 (European Research Council) under grant agreement no ERC-2016-CoG 725034. This work was further supported by the Associate Laboratory for Green Chemistry - LAQV and the project CryoDES which are financed by national funds from FCT/MCTES: UIDB/50006/2020 and PTDC/EQU–EQU/29851/2017, respectively. A.P. and L.M. also acknowledge FCT/MCTES for the financial support through IF/01146/2015 and SFRH/BD/148510/2019, respectively. The authors wish to thank Elisabete Ferreira from the BioLab, supported by the Applied Molecular Biosciences Research Unit-UCIBIO and the Associated Laboratory for Green Chemistry Research Unit–LAQV (UIDP/04378/2020, UIDB/04378/2020 and UIDB/50006/2020, UIDP/50006/2020, respectively). Publisher Copyright: © 2022 American Chemical Society. All rights reserved.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-26
2022-09-26T00:00:00Z
2023-11-29T22:29:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/160713
url http://hdl.handle.net/10362/160713
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2168-0485
PURE: 71539876
https://doi.org/10.1021/acssuschemeng.2c04045
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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