An HIV-1/HIV-2 Chimeric Envelope Glycoprotein Generates Binding and Neutralising Antibodies against HIV-1 and HIV-2 Isolates

Detalhes bibliográficos
Autor(a) principal: Taveira, Nuno
Data de Publicação: 2023
Outros Autores: Figueiredo, Inês, Calado, Rita, Martin, Francisco, Bártolo, Inês, Marcelino, José Maria, Borrego, Pedro, Cardoso, Fernando M H, Barroso, Helena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/61921
Resumo: © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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spelling An HIV-1/HIV-2 Chimeric Envelope Glycoprotein Generates Binding and Neutralising Antibodies against HIV-1 and HIV-2 IsolatesHIV vaccinechimeric envelope glycoproteinsvaccinia virusneutralizing antibodiesneutralizing epitopes© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).The development of immunogens that elicit broadly reactive neutralising antibodies (bNAbs) is the highest priority for an HIV vaccine. We have shown that a prime-boost vaccination strategy with vaccinia virus expressing the envelope glycoprotein gp120 of HIV-2 and a polypeptide comprising the envelope regions C2, V3 and C3 elicits bNAbs against HIV-2. We hypothesised that a chimeric envelope gp120 containing the C2, V3 and C3 regions of HIV-2 and the remaining parts of HIV-1 would elicit a neutralising response against HIV-1 and HIV-2. This chimeric envelope was synthesised and expressed in vaccinia virus. Balb/c mice primed with the recombinant vaccinia virus and boosted with an HIV-2 C2V3C3 polypeptide or monomeric gp120 from a CRF01_AG HIV-1 isolate produced antibodies that neutralised >60% (serum dilution 1:40) of a primary HIV-2 isolate. Four out of nine mice also produced antibodies that neutralised at least one HIV-1 isolate. Neutralising epitope specificity was assessed using a panel of HIV-1 TRO.11 pseudoviruses with key neutralising epitopes disrupted by alanine substitution (N160A in V2; N278A in the CD4 binding site region; N332A in the high mannose patch). The neutralisation of the mutant pseudoviruses was reduced or abolished in one mouse, suggesting that neutralising antibodies target the three major neutralising epitopes in the HIV-1 envelope gp120. These results provide proof of concept for chimeric HIV-1/HIV-2 envelope glycoproteins as vaccine immunogens that can direct the antibody response against neutralising epitopes in the HIV-1 and HIV-2 surface glycoproteins.This work was funded by national funds through the FCT-Foundation for Science and Technology, I.P., under the project UIDB/04585/2020, by FCT and Aga Khan Development Network (AKDN)—Portugal Collaborative Research Network in Portuguese speaking countries in Africa, project 332821690 and by Ministry of Health, Portugal, project HIV/SAU/0008.11.MDPIRepositório da Universidade de LisboaTaveira, NunoFigueiredo, InêsCalado, RitaMartin, FranciscoBártolo, InêsMarcelino, José MariaBorrego, PedroCardoso, Fernando M HBarroso, Helena2024-01-18T18:25:32Z2023-05-222023-10-25T13:01:58Z2023-05-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/61921engTaveira N, Figueiredo I, Calado R, Martin F, Bártolo I, Marcelino JM, et al. An hiv-1/hiv-2 chimeric envelope glycoprotein generates binding and neutralising antibodies against hiv-1 and hiv-2 isolates. IJMS [Internet]. 22 de maio de 2023;24(10):9077. Disponível em: https://www.mdpi.com/1422-0067/24/10/9077cv-prod-335944610.3390/ijms24109077info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-22T01:20:26Zoai:repositorio.ul.pt:10451/61921Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:56:16.550670Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv An HIV-1/HIV-2 Chimeric Envelope Glycoprotein Generates Binding and Neutralising Antibodies against HIV-1 and HIV-2 Isolates
title An HIV-1/HIV-2 Chimeric Envelope Glycoprotein Generates Binding and Neutralising Antibodies against HIV-1 and HIV-2 Isolates
spellingShingle An HIV-1/HIV-2 Chimeric Envelope Glycoprotein Generates Binding and Neutralising Antibodies against HIV-1 and HIV-2 Isolates
Taveira, Nuno
HIV vaccine
chimeric envelope glycoproteins
vaccinia virus
neutralizing antibodies
neutralizing epitopes
title_short An HIV-1/HIV-2 Chimeric Envelope Glycoprotein Generates Binding and Neutralising Antibodies against HIV-1 and HIV-2 Isolates
title_full An HIV-1/HIV-2 Chimeric Envelope Glycoprotein Generates Binding and Neutralising Antibodies against HIV-1 and HIV-2 Isolates
title_fullStr An HIV-1/HIV-2 Chimeric Envelope Glycoprotein Generates Binding and Neutralising Antibodies against HIV-1 and HIV-2 Isolates
title_full_unstemmed An HIV-1/HIV-2 Chimeric Envelope Glycoprotein Generates Binding and Neutralising Antibodies against HIV-1 and HIV-2 Isolates
title_sort An HIV-1/HIV-2 Chimeric Envelope Glycoprotein Generates Binding and Neutralising Antibodies against HIV-1 and HIV-2 Isolates
author Taveira, Nuno
author_facet Taveira, Nuno
Figueiredo, Inês
Calado, Rita
Martin, Francisco
Bártolo, Inês
Marcelino, José Maria
Borrego, Pedro
Cardoso, Fernando M H
Barroso, Helena
author_role author
author2 Figueiredo, Inês
Calado, Rita
Martin, Francisco
Bártolo, Inês
Marcelino, José Maria
Borrego, Pedro
Cardoso, Fernando M H
Barroso, Helena
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Taveira, Nuno
Figueiredo, Inês
Calado, Rita
Martin, Francisco
Bártolo, Inês
Marcelino, José Maria
Borrego, Pedro
Cardoso, Fernando M H
Barroso, Helena
dc.subject.por.fl_str_mv HIV vaccine
chimeric envelope glycoproteins
vaccinia virus
neutralizing antibodies
neutralizing epitopes
topic HIV vaccine
chimeric envelope glycoproteins
vaccinia virus
neutralizing antibodies
neutralizing epitopes
description © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
publishDate 2023
dc.date.none.fl_str_mv 2023-05-22
2023-10-25T13:01:58Z
2023-05-22T00:00:00Z
2024-01-18T18:25:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/61921
url http://hdl.handle.net/10451/61921
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Taveira N, Figueiredo I, Calado R, Martin F, Bártolo I, Marcelino JM, et al. An hiv-1/hiv-2 chimeric envelope glycoprotein generates binding and neutralising antibodies against hiv-1 and hiv-2 isolates. IJMS [Internet]. 22 de maio de 2023;24(10):9077. Disponível em: https://www.mdpi.com/1422-0067/24/10/9077
cv-prod-3359446
10.3390/ijms24109077
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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